C57BL/6JCya-Loxl2em1/Cya
Common Name
Loxl2-KO
Product ID
S-KO-18109
Backgroud
C57BL/6JCya
Strain ID
KOCMP-94352-Loxl2-B6J-VA
Status
When using this mouse strain in a publication, please cite “Loxl2-KO Mouse (Catalog S-KO-18109) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Loxl2-KO
Strain ID
KOCMP-94352-Loxl2-B6J-VA
Gene Name
Product ID
S-KO-18109
Gene Alias
1110004B06Rik, 4930526G11Rik, 9430067E15Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 14
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000100420
NCBI RefSeq
NM_033325
Target Region
Exon 4~6
Size of Effective Region
~6.8 kb
Overview of Gene Research
Loxl2, or Lysyl Oxidase Like 2, is a copper and lysine tyrosyl-quinone (LTQ)-dependent amine oxidase belonging to the lysyl oxidase (LOX) family. Its canonical function is to catalyze the cross-linking of elastin and collagen in the extracellular matrix (ECM) [2]. It is involved in multiple biological processes, and its dysregulation is associated with various diseases [1-8]. Genetically modified mouse models with silenced or overexpressed Loxl2 have enabled in-depth exploration of its in vivo role [1].
In pancreatic ductal adenocarcinoma (PDAC), Loxl2 ablation in mouse models (KPCL2KO or KCL2KO) had little effect on primary tumour development but significantly decreased metastasis and increased overall survival, mainly through non-cell autonomous factors like ECM remodelling. Conversely, Loxl2 overexpression (KPCL2KI or KCL2KI) promoted primary and metastatic tumour growth and decreased overall survival, linked to increased epithelial-mesenchymal transition (EMT) and stemness. Tumour-associated macrophage-secreted oncostatin M (OSM) was identified as an inducer of Loxl2 expression [3].
In conclusion, Loxl2 plays a crucial role in regulating ECM cross-linking. Model-based research, especially using Loxl2 KO mouse models in PDAC, has revealed its significant role in tumour metastasis and overall survival, highlighting its potential as a therapeutic target in cancer treatment.
References：
1. Cano, Amparo, Eraso, Pilar, Mazón, María J, Portillo, Francisco. 2023. LOXL2 in Cancer: A Two-Decade Perspective. In International journal of molecular sciences, 24, . doi:10.3390/ijms241814405. https://pubmed.ncbi.nlm.nih.gov/37762708/
2. Wen, Bing, Xu, Li-Yan, Li, En-Min. 2020. LOXL2 in cancer: regulation, downstream effectors and novel roles. In Biochimica et biophysica acta. Reviews on cancer, 1874, 188435. doi:10.1016/j.bbcan.2020.188435. https://pubmed.ncbi.nlm.nih.gov/32976981/
3. Alonso-Nocelo, Marta, Ruiz-Cañas, Laura, Sancho, Patricia, Cano, Amparo, Sainz, Bruno. 2022. Macrophages direct cancer cells through a LOXL2-mediated metastatic cascade in pancreatic ductal adenocarcinoma. In Gut, 72, 345-359. doi:10.1136/gutjnl-2021-325564. https://pubmed.ncbi.nlm.nih.gov/35428659/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.