C57BL/6JCya-Bub1bem1/Cya
Common Name:
Bub1b-KO
Product ID:
S-KO-18111
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Bub1b-KO
Strain ID
KOCMP-12236-Bub1b-B6J-VA
Gene Name
Product ID
S-KO-18111
Gene Alias
BUBR1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Bub1bem1/Cya mice (Catalog S-KO-18111) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000038341
NCBI RefSeq
NM_009773
Target Region
Exon 3~4
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
BUB1b, also known as BUB1 mitotic checkpoint serine/threonine kinase B, is a crucial gene involved in the mitotic spindle assembly checkpoint. It plays an essential role in ensuring accurate chromosome segregation during cell division. This gene is associated with multiple signaling pathways that are vital for cell cycle regulation. Its normal function is of great biological importance as any disruption can lead to chromosomal instability and potential carcinogenesis [3].
In various cancers, BUB1b has been shown to act as an oncogene. In lung adenocarcinoma, its up-regulation in tumor tissues contributes to poor prognosis, and it enhances cell viability by forming a complex with OTUD3 and NRF2, stabilizing the NRF2 signaling pathway and conferring resistance to ferroptosis and chemotherapy [1]. In pancreatic ductal adenocarcinoma, histone lactylation enriches at the promoters of BUB1B and activates its transcription, and BUB1B along with TTK forms a positive feedback loop with glycolysis-histone lactylation, driving oncogenesis [2]. In prostate cancer, BUB1B monoallelic germline variants decrease BubR1 expression/stability, trigger chromosomal instability, and lead to resistance to Taxol-based therapies [3]. In extrahepatic cholangiocarcinoma, BUB1B promotes cancer progression via the JNK/c-Jun pathways [4]. In multiple myeloma, BUB1B overexpression facilitates cellular proliferation, induces drug resistance, and evokes chromosomal instability, and the circular RNA circBUB1B_544aa containing its kinase catalytic center has a synergistic effect [5]. In hepatocellular carcinoma, BUB1B promotes malignancy by activating the mTORC1 signaling pathway [6]. In lung adenocarcinoma, it promotes cancer progression by interacting with ZNF143 and regulating glycolysis [7]. In prostate cancer, it promotes cell proliferation via transcriptional regulation of MELK [8]. In gastric cancer, it promotes cisplatin resistance via Rad51-mediated DNA damage repair [9]. In bladder cancer, increased BUB1B/BUBR1 expression contributes to aberrant DNA repair activity and resistance to DNA-damaging agents [10].
In conclusion, BUB1b is a key gene in cell cycle regulation, and its dysregulation is strongly associated with the development and progression of multiple cancers. Studies using gene-editing techniques in cell lines and in vivo models, such as gene knockout (KO) or conditional knockout (CKO) mouse models, have been instrumental in uncovering its oncogenic role in these diseases, providing potential therapeutic targets and biomarkers for prognosis.
References:
1. Ding, Yanguang, Gao, Jian, Chen, Jun, Tong, Xin, Zhao, Jun. 2024. BUB1b impairs chemotherapy sensitivity via resistance to ferroptosis in lung adenocarcinoma. In Cell death & disease, 15, 525. doi:10.1038/s41419-024-06914-0. https://pubmed.ncbi.nlm.nih.gov/39043653/
2. Li, Fei, Si, Wenzhe, Xia, Li, Hong, Tianpei, Wei, Rui. 2024. Positive feedback regulation between glycolysis and histone lactylation drives oncogenesis in pancreatic ductal adenocarcinoma. In Molecular cancer, 23, 90. doi:10.1186/s12943-024-02008-9. https://pubmed.ncbi.nlm.nih.gov/38711083/
3. Silva, Maria P, Ferreira, Luísa T, Brás, Natércia F, Teixeira, Manuel R, Paulo, Paula. 2024. BUB1B monoallelic germline variants contribute to prostate cancer predisposition by triggering chromosomal instability. In Journal of biomedical science, 31, 74. doi:10.1186/s12929-024-01056-z. https://pubmed.ncbi.nlm.nih.gov/39014450/
4. Jiao, Chen Yu, Feng, Qin Chao, Li, Chang Xian, Wang, Xuehao, Li, Xiang Cheng. 2021. BUB1B promotes extrahepatic cholangiocarcinoma progression via JNK/c-Jun pathways. In Cell death & disease, 12, 63. doi:10.1038/s41419-020-03234-x. https://pubmed.ncbi.nlm.nih.gov/33431813/
5. Tang, Xiaozhu, Guo, Mengjie, Ding, Pinggang, Gu, Xiaosong, Yang, Ye. 2021. BUB1B and circBUB1B_544aa aggravate multiple myeloma malignancy through evoking chromosomal instability. In Signal transduction and targeted therapy, 6, 361. doi:10.1038/s41392-021-00746-6. https://pubmed.ncbi.nlm.nih.gov/34620840/
6. Qiu, Jiannan, Zhang, Shaopeng, Wang, Peng, Rao, Jianhua, Lu, Ling. 2020. BUB1B promotes hepatocellular carcinoma progression via activation of the mTORC1 signaling pathway. In Cancer medicine, 9, 8159-8172. doi:10.1002/cam4.3411. https://pubmed.ncbi.nlm.nih.gov/32977361/
7. Zhou, Xiaolei, Yuan, Yanli, Kuang, Hongping, Zhang, Hui, Zhang, Manlin. . BUB1B (BUB1 Mitotic Checkpoint Serine/Threonine Kinase B) promotes lung adenocarcinoma by interacting with Zinc Finger Protein ZNF143 and regulating glycolysis. In Bioengineered, 13, 2471-2485. doi:10.1080/21655979.2021.2013108. https://pubmed.ncbi.nlm.nih.gov/35068350/
8. Tian, Juan-Hua, Mu, Li-Jun, Wang, Mei-Yu, Bai, Xiao-Jing, Du, Yue-Feng. . BUB1B Promotes Proliferation of Prostate Cancer via Transcriptional Regulation of MELK. In Anti-cancer agents in medicinal chemistry, 20, 1140-1146. doi:10.2174/1871520620666200101141934. https://pubmed.ncbi.nlm.nih.gov/31893996/
9. Qin, Zhe, Ye, Fangzhou, Wang, Jiayi, Li, Huanqing, Feng, Li. 2025. BUB1B promotes cisplatin resistance in gastric cancer via Rad51-mediated DNA damage repair. In Translational oncology, 54, 102334. doi:10.1016/j.tranon.2025.102334. https://pubmed.ncbi.nlm.nih.gov/40056529/
10. Komura, Kazumasa, Inamoto, Teruo, Tsujino, Takuya, Ono, Fumihito, Azuma, Haruhito. 2021. Increased BUB1B/BUBR1 expression contributes to aberrant DNA repair activity leading to resistance to DNA-damaging agents. In Oncogene, 40, 6210-6222. doi:10.1038/s41388-021-02021-y. https://pubmed.ncbi.nlm.nih.gov/34545188/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen