C57BL/6JCya-Nfatc2ipem1/Cya
Common Name:
Nfatc2ip-KO
Product ID:
S-KO-18114
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Nfatc2ip-KO
Strain ID
KOCMP-18020-Nfatc2ip-B6J-VB
Gene Name
Product ID
S-KO-18114
Gene Alias
D7Ertd304e; NIP45
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nfatc2ipem1/Cya mice (Catalog S-KO-18114) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000075671
NCBI RefSeq
NM_010900.3
Target Region
Exon 3~5
Size of Effective Region
~3.6 kb
Detailed Document
Overview of Gene Research
Nfatc2ip, also known as NIP45, is an evolutionarily conserved protein with tandem SUMO-like domains. It is crucial for SUMO-dependent genome integrity. It acts in interphase, associates with nascent DNA, and is involved in post-replicative resolution of replication or recombination intermediates. It interacts with the SMC5/6 complex and UBC9 (SUMO E2) via its SUMO-like domains, and is part of pathways related to genome duplication, repair, and cell proliferation [1,3].
Cells lacking Nfatc2ip are viable but hypersensitive to SUMO E1 inhibition, showing mitotic chromosome bridges and micronuclei, suggesting its key role in maintaining genomic integrity [1]. In breast cancer, TRPV6 activates NFATC2 by increasing Nfatc2ip phosphorylation at Ser204, promoting breast cancer metastasis [2]. In rainbow trout, miR-301b-5p and Nfatc2ip regulate inflammatory responses in the liver under high-temperature stress [5]. In myocardial hypertrophy, miR-31-5p targets Nfatc2ip to inhibit the process [4].
In summary, Nfatc2ip is essential for SUMO-dependent genome integrity. Its study through loss-of-function models has revealed its roles in various biological processes and disease conditions such as cancer metastasis, inflammatory responses, and myocardial hypertrophy. Understanding Nfatc2ip provides insights into the underlying mechanisms of these biological events and potential therapeutic targets.
References：
1. Cho, Tiffany, Hoeg, Lisa, Setiaputra, Dheva, Durocher, Daniel. 2024. NFATC2IP is a mediator of SUMO-dependent genome integrity. In Genes & development, 38, 233-252. doi:10.1101/gad.350914.123. https://pubmed.ncbi.nlm.nih.gov/38503515/
2. Xu, Xiang, Li, Na, Wang, Yugang, Yu, Jinming, Mi, Jun. 2021. Calcium channel TRPV6 promotes breast cancer metastasis by NFATC2IP. In Cancer letters, 519, 150-160. doi:10.1016/j.canlet.2021.07.017. https://pubmed.ncbi.nlm.nih.gov/34265397/
3. Hertz, Emil P T, Vega, Ignacio Alonso-de, Kruse, Thomas, Hickson, Ian D, Mailand, Niels. 2023. The SUMO-NIP45 pathway processes toxic DNA catenanes to prevent mitotic failure. In Nature structural & molecular biology, 30, 1303-1313. doi:10.1038/s41594-023-01045-0. https://pubmed.ncbi.nlm.nih.gov/37474739/
4. Zhao, Lamei, Qian, Xiaotao, Ren, Zhenxing, Wang, Ailing. . miR-31-5p suppresses myocardial hypertrophy by targeting Nfatc2ip. In Journal of cellular and molecular medicine, 28, e18413. doi:10.1111/jcmm.18413. https://pubmed.ncbi.nlm.nih.gov/38894694/
5. Huang, Tianqing, Gu, Wei, Liu, Enhui, Li, Shanwei, Xu, Gefeng. 2022. miR-301b-5p and its target gene nfatc2ip regulate inflammatory responses in the liver of rainbow trout (Oncorhynchus mykiss) under high temperature stress. In Ecotoxicology and environmental safety, 242, 113915. doi:10.1016/j.ecoenv.2022.113915. https://pubmed.ncbi.nlm.nih.gov/35901591/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen