C57BL/6JCya-Spibem1/Cya
Common Name:
Spib-KO
Product ID:
S-KO-18116
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Spib-KO
Strain ID
KOCMP-272382-Spib-B6J-VB
Gene Name
Product ID
S-KO-18116
Gene Alias
Spi-B
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Spibem1/Cya mice (Catalog S-KO-18116) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000035323
NCBI RefSeq
NM_019866.1
Target Region
Exon 2~5
Size of Effective Region
~3.1 kb
Detailed Document
Overview of Gene Research
SpiB, also known as Spi-B transcription factor, is a member of the E-twenty-six (ETS) transcription factor family. It is essential for the survival of mature B cells and participates in regulating various biological processes. SpiB is involved in multiple signaling pathways such as JAK1/STAT6, NF-κB, and JNK, and plays a significant role in B-cell-related functions, tumor development, and immune responses [1,3,4]. Mouse models, including gene knockout (KO) and conditional knockout (CKO) models, are valuable for studying its functions in vivo.
In IgG1+ cell-specific SpiB conditional knockout (cKO) mice, SpiB was found to be critical for the generation of late memory B cells but not early memory B cells or GC B cells. It suppresses plasma cell differentiation by regulating the expression of Blimp1 and Bach2, and upregulates anti-apoptosis and autophagy genes to control the survival of memory B cells [1]. In lung cancer cells, knockdown of SpiB by short hairpin RNA abrogated SNAP47 transcriptional activation upon matrix deprivation, indicating its role in autophagy-mediated anoikis resistance [2]. In ovarian cancer cells, SpiB knockdown inhibited cell proliferation, migration, and invasion, and boosted apoptosis by downregulating PD-L1 and inactivating the JAK/STAT pathway [3]. In colorectal cancer cells, overexpression of SpiB inhibited cell proliferation, migration, invasion, and angiogenesis, and induced cell cycle arrest and apoptosis. It also sensitized cells to oxaliplatin and 5-FU by activating the NF-κB and JNK signaling pathways through MAP4K1 [4]. In the placenta, down-regulated Prdx2 in recurrent miscarriage suppresses the SPIB-HDAC2 pathway, restraining trophoblast proliferation and migration [5]. In thymic microfold TECs, SpiB is required for their development and is essential for the generation of thymic IgA+ plasma cells [6].
In conclusion, SpiB has diverse functions in B-cell development, memory B-cell maintenance, and tumor-related processes. Mouse KO/CKO models have revealed its significance in these areas, contributing to a better understanding of the underlying mechanisms in diseases such as cancer, immune-related disorders, and pregnancy-associated pathologies.
References:
1. Horiuchi, Shu, Koike, Takuya, Takebuchi, Hirofumi, Kaisho, Tsuneyasu, Kitamura, Daisuke. 2023. SpiB regulates the expression of B-cell-related genes and increases the longevity of memory B cells. In Frontiers in immunology, 14, 1250719. doi:10.3389/fimmu.2023.1250719. https://pubmed.ncbi.nlm.nih.gov/37965309/
2. Zhang, Hua, Wang, Guobin, Zhou, Ruimin, Liu, Zhe, Ma, Zhenyi. 2020. SPIB promotes anoikis resistance via elevated autolysosomal process in lung cancer cells. In The FEBS journal, 287, 4696-4709. doi:10.1111/febs.15272. https://pubmed.ncbi.nlm.nih.gov/32129936/
3. Gu, Wenfei, Qi, GuangTao, Chen, Li. . SPIB Knockdown Inhibits the Immune Escape of Ovarian Cancer Cells by Reducing PD-L1 (CD274) Expression and Inactivating the JAK/STAT Pathway. In Iranian journal of immunology : IJI, 20, 335-347. doi:10.22034/iji.2023.98236.2559. https://pubmed.ncbi.nlm.nih.gov/37452634/
4. Zhao, Xunping, Li, Lin, Yuan, Shiyun, Jiang, Xianyao, Luo, Tao. 2021. SPIB acts as a tumor suppressor by activating the NFkB and JNK signaling pathways through MAP4K1 in colorectal cancer cells. In Cellular signalling, 88, 110148. doi:10.1016/j.cellsig.2021.110148. https://pubmed.ncbi.nlm.nih.gov/34530056/
5. Wu, Fan, Tian, Fuju, Qin, Chuanmei, Chen, Cailian, Lin, Yi. 2022. Peroxiredoxin2 regulates trophoblast proliferation and migration through SPIB-HDAC2 pathway. In Experimental cell research, 422, 113428. doi:10.1016/j.yexcr.2022.113428. https://pubmed.ncbi.nlm.nih.gov/36400181/
6. Givony, Tal, Leshkowitz, Dena, Del Castillo, Diana, Goldfarb, Yael, Abramson, Jakub. 2023. Thymic mimetic cells function beyond self-tolerance. In Nature, 622, 164-172. doi:10.1038/s41586-023-06512-8. https://pubmed.ncbi.nlm.nih.gov/37674082/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen