C57BL/6JCya-Tirapem1/Cya
Common Name:
Tirap-KO
Product ID:
S-KO-18146
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tirap-KO
Strain ID
KOCMP-117149-Tirap-B6J-VB
Gene Name
Product ID
S-KO-18146
Gene Alias
C130027E04Rik; Mal; Tlr4ap; Wyatt
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tirapem1/Cya mice (Catalog S-KO-18146) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000175765
NCBI RefSeq
NM_001177845
Target Region
Exon 6~7
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Tirap, also known as Toll-interleukin-1 Receptor (TIR) domain-containing adaptor protein (TIRAP) or MAL, is a key intracellular signalling molecule regulating diverse immune responses [1,3]. It functions as an adaptor in Toll-like Receptor (TLR)-mediated innate immune signalling, mainly in the MyD88-dependent TLR signalling pathway, such as through TLR2 and TLR4 [1,4]. It also interacts with non-TLR signalling mediators, indicating its central role in various immune responses [1].
In a mouse model, Tirap heterozygous mice were more resistant to Mycobacterium tuberculosis (Mtb) infection compared to wild-type littermates. Mtb infection induced Tirap expression, which prevented phagosomal acidification and rupture, and the Tirap-mediated anti-tuberculosis effect occurred through a Cish-dependent signalling pathway [2]. In another study, overexpression of TIRAP in various types of myelodysplastic syndromes (MDS) suppressed all three major hematopoietic lineages. TIRAP expression promoted up-regulation of Ifnγ, leading to myelosuppression through Ifnγ-Ifnγr-mediated release of Hmgb1, which disrupted the bone marrow endothelial niche. Deletion of Ifnγ reversed the endothelial defect and restored myelopoiesis [5].
In conclusion, Tirap plays a crucial role in immune responses, especially in TLR-mediated signalling. Model-based research, such as gene-modified mouse models, has revealed its significance in diseases like tuberculosis and myelodysplastic syndromes. Understanding Tirap's functions provides insights into the pathophysiology of these diseases and may offer potential therapeutic targets.
References:
1. Rajpoot, Sajjan, Wary, Kishore K, Ibbott, Rachel, Thurston, Teresa L M, Baig, Mirza S. 2021. TIRAP in the Mechanism of Inflammation. In Frontiers in immunology, 12, 697588. doi:10.3389/fimmu.2021.697588. https://pubmed.ncbi.nlm.nih.gov/34305934/
2. Belhaouane, Imène, Pochet, Amine, Chatagnon, Jonathan, Brodin, Priscille, Machelart, Arnaud. 2023. Tirap controls Mycobacterium tuberculosis phagosomal acidification. In PLoS pathogens, 19, e1011192. doi:10.1371/journal.ppat.1011192. https://pubmed.ncbi.nlm.nih.gov/36888688/
3. Belhaouane, Imène, Hoffmann, Eik, Chamaillard, Mathias, Brodin, Priscille, Machelart, Arnaud. 2020. Paradoxical Roles of the MAL/Tirap Adaptor in Pathologies. In Frontiers in immunology, 11, 569127. doi:10.3389/fimmu.2020.569127. https://pubmed.ncbi.nlm.nih.gov/33072109/
4. Takeda, Kiyoshi, Akira, Shizuo. . TLR signaling pathways. In Seminars in immunology, 16, 3-9. doi:. https://pubmed.ncbi.nlm.nih.gov/14751757/
5. Gopal, Aparna, Ibrahim, Rawa, Fuller, Megan, Lu, Melody, Karsan, Aly. 2022. TIRAP drives myelosuppression through an Ifnγ-Hmgb1 axis that disrupts the endothelial niche in mice. In The Journal of experimental medicine, 219, . doi:10.1084/jem.20200731. https://pubmed.ncbi.nlm.nih.gov/35089323/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen