C57BL/6JCya-Dpep1em1/Cya
Common Name:
Dpep1-KO
Product ID:
S-KO-18158
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Dpep1-KO
Strain ID
KOCMP-13479-Dpep1-B6J-VB
Gene Name
Product ID
S-KO-18158
Gene Alias
DPEP-1; MBD
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dpep1em1/Cya mice (Catalog S-KO-18158) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000019422
NCBI RefSeq
NM_007876
Target Region
Exon 3~11
Size of Effective Region
~2.9 kb
Detailed Document
Overview of Gene Research
Dpep1, also known as Dipeptidase-1, is a zinc-dependent metalloproteinase. It functions as a major neutrophil adhesion receptor and is involved in leukocyte recruitment. In the kidney, it may be associated with the ferroptosis pathway, and in cancer cells, it is related to drug resistance and tumor progression pathways. Genetic models, especially KO mouse models, are valuable for studying its functions [1,2,4].
In Dpep1-/-mice, renal inflammation and acute kidney injury (AKI) phenotypes were attenuated during ischemia reperfusion injury (IRI). This indicates that Dpep1 deficiency blocks neutrophil adhesion to peritubular capillaries and reduces inflammatory monocyte recruitment to the kidney after IRI [1]. In addition, in vitro and in vivo assays in hepatoblastoma (HB) models showed that silencing Dpep1 significantly suppressed HB cell proliferation, migration, and invasion [3].
In conclusion, Dpep1 plays a crucial role in leukocyte recruitment, especially in the kidney during IRI, and also in the progression of certain cancers such as colon cancer, hepatoblastoma, and B-cell acute lymphoblastic leukaemia. The use of Dpep1 KO mouse models has been instrumental in uncovering its role in these disease-related biological processes [1,2,3,5].
References：
1. Lau, Arthur, Rahn, Jennifer J, Chappellaz, Mona, Chun, Justin, Muruve, Daniel A. 2022. Dipeptidase-1 governs renal inflammation during ischemia reperfusion injury. In Science advances, 8, eabm0142. doi:10.1126/sciadv.abm0142. https://pubmed.ncbi.nlm.nih.gov/35108057/
2. Zeng, Cheng, Qi, Guoping, Shen, Ying, Liu, Qian, Jin, Jianhua. 2022. DPEP1 promotes drug resistance in colon cancer cells by forming a positive feedback loop with ASCL2. In Cancer medicine, 12, 412-424. doi:10.1002/cam4.4926. https://pubmed.ncbi.nlm.nih.gov/35670012/
3. Cui, Xichun, Liu, Xin, Han, Qicai, Zhang, Da, Dong, Gang. 2019. DPEP1 is a direct target of miR-193a-5p and promotes hepatoblastoma progression by PI3K/Akt/mTOR pathway. In Cell death & disease, 10, 701. doi:10.1038/s41419-019-1943-0. https://pubmed.ncbi.nlm.nih.gov/31541079/
4. Guan, Yuting, Liang, Xiujie, Ma, Ziyuan, Voight, Benjamin F, Susztak, Katalin. 2021. A single genetic locus controls both expression of DPEP1/CHMP1A and kidney disease development via ferroptosis. In Nature communications, 12, 5078. doi:10.1038/s41467-021-25377-x. https://pubmed.ncbi.nlm.nih.gov/34426578/
5. Zhang, Jia-Min, Xu, Yan, Gale, Robert P, Liu, Kai-Yan, Ruan, Guo-Rui. 2020. DPEP1 expression promotes proliferation and survival of leukaemia cells and correlates with relapse in adults with common B cell acute lymphoblastic leukaemia. In British journal of haematology, 190, 67-78. doi:10.1111/bjh.16505. https://pubmed.ncbi.nlm.nih.gov/32068254/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen