C57BL/6JCya-Tmem168em1/Cya
Common Name:
Tmem168-KO
Product ID:
S-KO-18193
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tmem168-KO
Strain ID
KOCMP-101118-Tmem168-B6J-VB
Gene Name
Product ID
S-KO-18193
Gene Alias
5730526F17Rik; 8430437G11Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tmem168em1/Cya mice (Catalog S-KO-18193) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031554
NCBI RefSeq
NM_028990
Target Region
Exon 3~4
Size of Effective Region
~3.8 kb
Detailed Document
Overview of Gene Research
Tmem168, a protein-coding gene, consists of 697 amino acid residues with some putative transmembrane domains. It has been implicated in multiple biological processes and disease-related pathways. In the nucleus accumbens (NAcc), it is associated with drug-dependence regulation and interacts with osteopontin. It may also be involved in the Wnt/β-catenin pathway in glioblastoma cells [1,4,5].
In mice, overexpression of Tmem168 in the NAcc using an adeno-associated virus vector led to attenuation of methamphetamine-induced hyperlocomotion and place preference, and suppressed methamphetamine-induced extracellular dopamine elevation. Additionally, TMEM168 overexpression enhanced anxiety and caused sensorimotor gating deficit, with these effects reversible by relevant drugs. In a family with Brugada syndrome, a heterozygous 1616G > A substitution (R539Q mutation) in Tmem168 was identified. The mutant Tmem168 in HL-1 cardiomyocytes led to a significant decrease in Na+ current and Nav 1.5 protein expression. Heterozygous Tmem168 1616G > A knock-in mice showed ventricular tachyarrhythmias and conduction disorders upon pharmacological stimulation [1,3,4,2].
In conclusion, Tmem168 plays important roles in drug-dependence-related behaviors, anxiety-related phenotypes, and arrhythmogenesis. Mouse models, especially those with Tmem168 overexpression or mutation, have been crucial in revealing these functions, providing insights into potential therapeutic targets for methamphetamine dependence and Brugada syndrome.
References:
1. Nitta, Atsumi. . [Novel molecules-related drug dependence in mice]. In Nihon yakurigaku zasshi. Folia pharmacologica Japonica, 155, 140-144. doi:10.1254/fpj.19127. https://pubmed.ncbi.nlm.nih.gov/32378630/
2. Shimizu, Akio, Zankov, Dimitar P, Sato, Akira, Horie, Minoru, Ogita, Hisakazu. 2020. Identification of transmembrane protein 168 mutation in familial Brugada syndrome. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34, 6399-6417. doi:10.1096/fj.201902991R. https://pubmed.ncbi.nlm.nih.gov/32175648/
3. Fu, Kequan, Miyamoto, Yoshiaki, Sumi, Kazuyuki, Uno, Kyosuke, Nitta, Atsumi. 2017. Overexpression of transmembrane protein 168 in the mouse nucleus accumbens induces anxiety and sensorimotor gating deficit. In PloS one, 12, e0189006. doi:10.1371/journal.pone.0189006. https://pubmed.ncbi.nlm.nih.gov/29211814/
4. Fu, Kequan, Miyamoto, Yoshiaki, Otake, Kazuya, Muramatsu, Shin-Ichi, Nitta, Atsumi. 2017. Involvement of the accumbal osteopontin-interacting transmembrane protein 168 in methamphetamine-induced place preference and hyperlocomotion in mice. In Scientific reports, 7, 13084. doi:10.1038/s41598-017-13289-0. https://pubmed.ncbi.nlm.nih.gov/29026117/
5. Xu, Jie, Su, Zhongzhou, Ding, Qiuping, Li, Liqin, Lu, Bin. 2019. Inhibition of Proliferation by Knockdown of Transmembrane (TMEM) 168 in Glioblastoma Cells via Suppression of Wnt/β-Catenin Pathway. In Oncology research, 27, 819-826. doi:10.3727/096504018X15478559215014. https://pubmed.ncbi.nlm.nih.gov/30940290/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen