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C57BL/6JCya-Cc2d1aem1/Cya
Common Name:
Cc2d1a-KO
Product ID:
S-KO-18194
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Cc2d1a-KO
Strain ID
KOCMP-212139-Cc2d1a-B6J-VA
Gene Name
Cc2d1a
Product ID
S-KO-18194
Gene Alias
Freud-1; Tape
Background
C57BL/6JCya
NCBI ID
212139
Modification
Conventional knockout
Chromosome
8
Phenotype
MGI:2384831
Document
Click here to download >>
Application
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More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cc2d1aem1/Cya mice (Catalog S-KO-18194) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000040383
NCBI RefSeq
NM_145970
Target Region
Exon 7~14
Size of Effective Region
~3.9 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Cc2d1a, also known as Freud-1, belongs to a gene family with conserved domains including DM14, a helix-loop-helix domain, and a C2 calcium-dependent phospholipid binding domain. It functions as a transcriptional repressor of the serotonin-1A receptor gene and is involved in regulating multiple intracellular signaling pathways, with a particularly strong effect on the NF-κB pathway [4,8]. It is also associated with endosomal sorting pathways by interacting with CHMP4 family proteins, regulating degradation and signaling of transmembrane receptors like EGFR and TLR4 [7].

In KO/CKO mouse models, conditional deletion of Cc2d1a in glutamatergic neurons leads to autistic-like features such as self-injurious repetitive grooming, aberrant social interactions, decreased dendritic complexity, and increased synaptic excitation/inhibition ratio [2]. In male mice lacking Cc2d1a in forebrain excitatory neurons, there is increased irritability-like behavior, which is related to reduced oxytocin-expressing neurons in the hypothalamus [6]. Conditional deletion from excitatory neurons in male mouse forebrain also impairs hippocampal synaptic plasticity, cognitive function, and object location memory, and is associated with enhanced Rac1 activity [5]. Heterozygous Cc2d1a mice show sex-dependent autophagy impairment in the prefrontal cortex and hippocampus [3]. In Xenopus, loss of cc2d1a causes cardiac heterotaxy, cystic kidneys, and abnormal CSF circulation due to defective ciliogenesis [1].

In conclusion, Cc2d1a is crucial for normal brain development, ciliogenesis, and CSF circulation. Cc2d1a KO/CKO mouse models have revealed its roles in neurodevelopmental disorders such as autism spectrum disorder, intellectual disability, and related behavioral abnormalities, providing insights into the underlying mechanisms and potential therapeutic targets for these diseases.

References:
1. Kim, Angelina Haesoo, Sakin, Irmak, Viviano, Stephen, Temel, Sehime G, Deniz, Engin. 2024. CC2D1A causes ciliopathy, intellectual disability, heterotaxy, renal dysplasia, and abnormal CSF flow. In Life science alliance, 7, . doi:10.26508/lsa.202402708. https://pubmed.ncbi.nlm.nih.gov/39168639/
2. Yang, Cheng-Yi, Hung, Yu-Chieh, Cheng, Kuan-Hsiang, Ling, Pin, Hsu, Kuei-Sen. 2021. Loss of CC2D1A in Glutamatergic Neurons Results in Autistic-Like Features in Mice. In Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics, 18, 2021-2039. doi:10.1007/s13311-021-01072-z. https://pubmed.ncbi.nlm.nih.gov/34132974/
3. Sener, Elif Funda, Dana, Halime, Tahtasakal, Reyhan, Ozkul, Yusuf, Rassoulzadegan, Minoo. 2023. Heterozygous Cc2d1a mice show sex-dependent changes in the Beclin-1/p62 ratio with impaired prefrontal cortex and hippocampal autophagy. In Progress in neuro-psychopharmacology & biological psychiatry, 125, 110764. doi:10.1016/j.pnpbp.2023.110764. https://pubmed.ncbi.nlm.nih.gov/37059290/
4. Rogaeva, Anastasia, Galaraga, Kimberly, Albert, Paul R. . The Freud-1/CC2D1A family: transcriptional regulators implicated in mental retardation. In Journal of neuroscience research, 85, 2833-8. doi:. https://pubmed.ncbi.nlm.nih.gov/17394259/
5. Yang, Cheng-Yi, Yu, Ting-Hsuan, Wen, Wan-Ling, Ling, Pin, Hsu, Kuei-Sen. 2019. Conditional Deletion of CC2D1A Reduces Hippocampal Synaptic Plasticity and Impairs Cognitive Function through Rac1 Hyperactivation. In The Journal of neuroscience : the official journal of the Society for Neuroscience, 39, 4959-4975. doi:10.1523/JNEUROSCI.2395-18.2019. https://pubmed.ncbi.nlm.nih.gov/30992372/
6. Cheng, Kuan-Hsiang, Hung, Yu-Chieh, Ling, Pin, Hsu, Kuei-Sen. 2024. Oxytocin treatment rescues irritability-like behavior in Cc2d1a conditional knockout mice. In Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 49, 1792-1802. doi:10.1038/s41386-024-01920-4. https://pubmed.ncbi.nlm.nih.gov/39014123/
7. Deshar, Rakesh, Cho, Eun-Bee, Yoon, Sungjoo Kim, Yoon, Jong-Bok. 2016. CC2D1A and CC2D1B regulate degradation and signaling of EGFR and TLR4. In Biochemical and biophysical research communications, 480, 280-287. doi:10.1016/j.bbrc.2016.10.053. https://pubmed.ncbi.nlm.nih.gov/27769858/
8. Manzini, M Chiara, Xiong, Lan, Shaheen, Ranad, Alkuraya, Fowzan S, Walsh, Christopher A. 2014. CC2D1A regulates human intellectual and social function as well as NF-κB signaling homeostasis. In Cell reports, 8, 647-55. doi:10.1016/j.celrep.2014.06.039. https://pubmed.ncbi.nlm.nih.gov/25066123/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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