C57BL/6JCya-Sox10em1/Cya
Common Name:
Sox10-KO
Product ID:
S-KO-18196
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Sox10-KO
Strain ID
KOCMP-20665-Sox10-B6J-VA
Gene Name
Product ID
S-KO-18196
Gene Alias
Dom; Sox21; gt
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sox10em1/Cya mice (Catalog S-KO-18196) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000040019
NCBI RefSeq
NM_011437.1
Target Region
Exon 3
Size of Effective Region
~269 bp
Detailed Document
Overview of Gene Research
SOX10, belonging to a family of 20 SRY-related high mobility group box-containing (SOX) proteins, is crucial for cell type specification and differentiation, especially in neural crest development [1]. It encodes a transcription factor involved in embryogenesis and cell differentiation, aiding neural crest shuttling and development [2].
In mouse models, Sox10 upregulation is associated with macrophage-like vascular smooth muscle cell (VSMC) accumulation and pyroptosis in neointimal hyperplasia, suggesting it escalates vascular inflammation [3]. In glioblastoma, suppressing Sox10 promotes tumor progression to an aggressive neural stem-cell (NSC)/developmental-like phenotype, including a quiescent NSC-like cell population [4]. In melanoma, loss of SOX10 reduces proliferation, leads to invasive properties, and promotes tolerance to BRAF and/or MEK inhibitors [5]. Also, SOX10-deficient melanoma cells are more sensitive to CD8+ T cell-mediated killing and cytokine-induced cell death [7]. In schwannomas, novel SOX10 indel mutations drive a unique subtype by impeding proper differentiation of immature Schwann cells [6].
In conclusion, SOX10 is essential for the development and differentiation of various cell lineages, especially those derived from the neural crest. Model-based research, especially gene knockout studies in mice, has revealed its role in multiple disease conditions such as vascular inflammation, glioblastoma, melanoma, and schwannomas, providing insights into disease mechanisms and potential therapeutic targets.
References:
1. Pingault, Veronique, Zerad, Lisa, Bertani-Torres, William, Bondurand, Nadege. 2021. SOX10: 20 years of phenotypic plurality and current understanding of its developmental function. In Journal of medical genetics, 59, 105-114. doi:10.1136/jmedgenet-2021-108105. https://pubmed.ncbi.nlm.nih.gov/34667088/
2. Sy, Albert L, Hoang, Mai P. 2023. SOX10. In Journal of clinical pathology, 76, 649-653. doi:10.1136/jcp-2023-208924. https://pubmed.ncbi.nlm.nih.gov/37336549/
3. Xu, Xin, Zhang, Dan-Dan, Kong, Peng, Zhang, Fan, Han, Mei. 2023. Sox10 escalates vascular inflammation by mediating vascular smooth muscle cell transdifferentiation and pyroptosis in neointimal hyperplasia. In Cell reports, 42, 112869. doi:10.1016/j.celrep.2023.112869. https://pubmed.ncbi.nlm.nih.gov/37481722/
4. Man, Ka-Hou, Wu, Yonghe, Gao, Zhenjiang, Lichter, Peter, Radlwimmer, Bernhard. 2024. SOX10 mediates glioblastoma cell-state plasticity. In EMBO reports, 25, 5113-5140. doi:10.1038/s44319-024-00258-8. https://pubmed.ncbi.nlm.nih.gov/39285246/
5. Capparelli, Claudia, Purwin, Timothy J, Glasheen, McKenna, Herlyn, Meenhard, Aplin, Andrew E. 2022. Targeting SOX10-deficient cells to reduce the dormant-invasive phenotype state in melanoma. In Nature communications, 13, 1381. doi:10.1038/s41467-022-28801-y. https://pubmed.ncbi.nlm.nih.gov/35296667/
6. Williams, Erik A, Ravindranathan, Ajay, Gupta, Rohit, Costello, Joseph F, Solomon, David A. . Novel SOX10 indel mutations drive schwannomas through impaired transactivation of myelination gene programs. In Neuro-oncology, 25, 2221-2236. doi:10.1093/neuonc/noad121. https://pubmed.ncbi.nlm.nih.gov/37436963/
7. Rosenbaum, Sheera R, Caksa, Signe, Stefanski, Casey D, Capparelli, Claudia, Aplin, Andrew E. . SOX10 Loss Sensitizes Melanoma Cells to Cytokine-Mediated Inflammatory Cell Death. In Molecular cancer research : MCR, 22, 209-220. doi:10.1158/1541-7786.MCR-23-0290. https://pubmed.ncbi.nlm.nih.gov/37847239/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen