C57BL/6JCya-Cfl1em1/Cya
Common Name
Cfl1-KO
Product ID
S-KO-18214
Backgroud
C57BL/6JCya
Strain ID
KOCMP-12631-Cfl1-B6J-VA
When using this mouse strain in a publication, please cite “Cfl1-KO Mouse (Catalog S-KO-18214) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Cfl1-KO
Strain ID
KOCMP-12631-Cfl1-B6J-VA
Gene Name
Product ID
S-KO-18214
Gene Alias
Cof
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 19
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000209469
NCBI RefSeq
NM_007687
Target Region
Exon 2~4
Size of Effective Region
~2.6 kb
Overview of Gene Research
Cfl1, also known as cofilin, is a critical member of the actin depolymerizing factor (ADF) family. It plays a significant role in regulating actin cytoskeleton dynamics by cutting and depolymerizing actin filaments, which is essential for various biological processes such as cell migration, proliferation, and immune responses [2].
In chronic myeloid leukemia, the expression of Cfl1 was lower in patients compared to healthy controls and was upregulated after imatinib therapy. Lower Cfl1 expression before treatment was associated with a better response to imatinib, suggesting its potential as a predictor [1]. In hepatocellular carcinoma, Cfl1 is highly expressed in sorafenib-insensitive patients, and its knockdown can enhance the sensitivity of the cancer cells to sorafenib by remodeling serine synthesis and metabolism [3]. In gastric cancer, Cfl1 expression is increased, and its silencing by siRNA can inhibit cell migration and increase apoptosis, indicating it may function as an oncogenic gene [4].
In conclusion, Cfl1 is crucial for regulating actin cytoskeleton-related biological processes. Its study in disease models, such as in leukemia, liver cancer, and gastric cancer, has revealed its potential as a biomarker and therapeutic target, providing valuable insights into disease mechanisms and treatment strategies.
References：
1. Yin, Xiufeng, Li, Xia, Jiang, Hao, Zhang, Jin, Jin, Jie. 2024. CFL1 is Implicated in Chronic Myeloid Leukemia Response during Imatinib Therapy. In Journal of Cancer, 15, 2424-2430. doi:10.7150/jca.92202. https://pubmed.ncbi.nlm.nih.gov/38495482/
2. Xing, Jianxiao, Wang, Ying, Peng, Aihong, Niu, Xuping, Zhang, Kaiming. 2024. The role of actin cytoskeleton CFL1 and ADF/cofilin superfamily in inflammatory response. In Frontiers in molecular biosciences, 11, 1408287. doi:10.3389/fmolb.2024.1408287. https://pubmed.ncbi.nlm.nih.gov/39114368/
3. Li, Senlin, Xu, Lei, Wu, Guo, Zhang, Lei, Xu, Xiaoding. 2023. Remodeling Serine Synthesis and Metabolism via Nanoparticles (NPs)-Mediated CFL1 Silencing to Enhance the Sensitivity of Hepatocellular Carcinoma to Sorafenib. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2207118. doi:10.1002/advs.202207118. https://pubmed.ncbi.nlm.nih.gov/37203277/
4. Daryabari, Seyedeh Saideh, Fathi, Marziyeh, Mahdavi, Majid, Shokoohi, Behrouz, Safaralizadeh, Reza. 2020. Overexpression of CFL1 in gastric cancer and the effects of its silencing by siRNA with a nanoparticle delivery system in the gastric cancer cell line. In Journal of cellular physiology, 235, 6660-6672. doi:10.1002/jcp.29562. https://pubmed.ncbi.nlm.nih.gov/31990066/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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