C57BL/6JCya-Tars3em1/Cya
Common Name
Tars3-KO
Product ID
S-KO-18259
Backgroud
C57BL/6JCya
Strain ID
KOCMP-272396-Tars3-B6J-VB
Status
When using this mouse strain in a publication, please cite “Tars3-KO Mouse (Catalog S-KO-18259) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Tars3-KO
Strain ID
KOCMP-272396-Tars3-B6J-VB
Gene Name
Product ID
S-KO-18259
Gene Alias
Tarsl2, A530046H20Rik
Background
C57BL/6JCya
Gene Full Name
threonyl-tRNA synthetase-like 2
Modification
Conventional knockout
NCBI ID
Phenotype
Chromosome
Chr 7 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000032728
NCBI Transcript ID
NM_172310
Target Region
Exon 2~3
Size of Effective Region
~1.4 kb
Overview of Gene Research
Tars3 is not mentioned in the provided references. However, TARSL2, a related gene, encodes a threonyl-tRNA synthetase-like protein (ThrRS-L) in higher eukaryotes. TARS and TARS2 encode cytoplasmic and mitochondrial threonyl-tRNA synthetases respectively, while TARSL2-encoded ThrRS-L has sequence homology to cytoplasmic ThrRS but a distinct N-terminal extension. ThrRS-L is involved in tRNA aminoacylation and editing, which are crucial processes in protein synthesis [1].
In glioma research, miR-720 was found to be upregulated in glioma tissues and cells. miR-720 promotes glioma migration and invasion by downregulating TARSL2. Reintroduction of TARSL2 into glioma cells can repress the invasion-promoting function of miR-720 [2]. Additionally, TARSL2 was identified as a potential member of the aminoacyl-tRNA synthetase core complex through affinity purification-mass spectrometry and co-immunoprecipitation experiments [3].
In conclusion, TARSL2 plays important roles in protein synthesis-related processes such as tRNA aminoacylation and editing. In glioma, its regulation by miR-720 is significant for understanding glioma migration and invasion. Identifying it as a potential component of the aminoacyl-tRNA synthetase core complex also provides new insights into the complex's function. The study of TARSL2 helps in understanding biological processes and disease mechanisms, potentially offering new strategies for glioma treatment.
References：
1. Chen, Yun, Ruan, Zhi-Rong, Wang, Yong, Zhou, Xiao-Long, Wang, En-Duo. . A threonyl-tRNA synthetase-like protein has tRNA aminoacylation and editing activities. In Nucleic acids research, 46, 3643-3656. doi:10.1093/nar/gky211. https://pubmed.ncbi.nlm.nih.gov/29579307/
2. Liu, Yinlong, Jiang, Kuan, Zhi, Tongle, Xu, Xiupeng. 2021. miR-720 is a key regulator of glioma migration and invasion by controlling TARSL2 expression. In Human cell, 34, 1504-1516. doi:10.1007/s13577-021-00551-x. https://pubmed.ncbi.nlm.nih.gov/34024034/
3. Kim, Kyutae, Park, Seong-Jun, Na, Seungjin, Paek, Eunok, Lee, Cheolju. 2013. Reinvestigation of aminoacyl-tRNA synthetase core complex by affinity purification-mass spectrometry reveals TARSL2 as a potential member of the complex. In PloS one, 8, e81734. doi:10.1371/journal.pone.0081734. https://pubmed.ncbi.nlm.nih.gov/24312579/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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