C57BL/6JCya-Ing3em1/Cya
Common Name:
Ing3-KO
Product ID:
S-KO-18283
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ing3-KO
Strain ID
KOCMP-71777-Ing3-B6J-VB
Gene Name
Product ID
S-KO-18283
Gene Alias
1300013A07Rik; P47ING3
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ing3em1/Cya mice (Catalog S-KO-18283) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031680
NCBI RefSeq
NM_023626
Target Region
Exon 3
Size of Effective Region
~0.1 kb
Detailed Document
Overview of Gene Research
ING3, a member of the ING family, is involved in regulating the transcriptional state of chromatin. It recruits remodeling complexes to sites with histone H3 trimethylated at Lysine 4 (H3K4me3) through its C-terminal Plant HomeoDomain (PHD), and facilitates histone acetylation by the NuA4-Tip60 MYST histone acetyl transferase complex. It plays crucial roles in cell cycle control, senescence, DNA repair, cell proliferation, and apoptosis, and is important for normal embryonic development [1,4,6,8].
A transgenic mouse strain with insertional mutation of an UbC-mCherry expression cassette into the endogenous Ing3 locus led to disruption of ING3 protein expression. Homozygous mutants were embryonically lethal, showing growth retardation and severe developmental disorders, especially in neural tube closure and primary brain vesicle formation, indicating ING3 is essential for prenatal brain formation [8]. In human cells, ING3-depleted cells are sensitive to DNA damage, as ING3 is recruited to DNA double-strand breaks and required for ATM activation, which is crucial for DNA repair by non-homologous end joining (NHEJ) or homologous recombination (HR), and for immunoglobulin class switch recombination (CSR) [6]. In prostate cancer, ING3 promotes cancer growth by activating the androgen receptor, while in other cancers like breast and lung adenocarcinoma, it acts as a tumor suppressor, inhibiting cell migration, invasion, and proliferation [2,3,5,7].
In conclusion, ING3 has diverse functions. Its role in DNA repair and embryonic development is revealed through gene-knockout mouse models. In disease, it can act as either a tumor suppressor or an oncogene depending on the cancer type. Studies on ING3 contribute to understanding cancer progression and potentially developing new cancer treatment strategies.
References:
1. Ferreras-Gutiérrez, Mariola, Chaves-Arquero, Belén, González-Magaña, Amaia, Medrano, Francisco J, Blanco, Francisco J. 2023. Structural analysis of ING3 protein and histone H3 binding. In International journal of biological macromolecules, 242, 124724. doi:10.1016/j.ijbiomac.2023.124724. https://pubmed.ncbi.nlm.nih.gov/37148949/
2. Wu, Xiaoyan, Chen, Chuang, Luo, Bin, Wu, Hao, Yuan, Jingping. 2021. Nuclear ING3 Expression Is Correlated With a Good Prognosis of Breast Cancer. In Frontiers in oncology, 10, 589009. doi:10.3389/fonc.2020.589009. https://pubmed.ncbi.nlm.nih.gov/33469513/
3. Li, Huimeng, Zhang, Hengyu, Tan, Xin, Liu, Rui, Tang, Shicong. 2021. Overexpression of ING3 is associated with attenuation of migration and invasion in breast cancer. In Experimental and therapeutic medicine, 22, 699. doi:10.3892/etm.2021.10131. https://pubmed.ncbi.nlm.nih.gov/34007308/
4. Martinez-Vargas, Yailit Del Carmen, Silva-Filho, Tiago João da, Oliveira, Denise Hélen Imaculada Pereira de, Gonçalo, Rani Iani Costa, Queiroz, Lélia Maria Guedes. . ING3 and ING4 immunoexpression and their relation to the development of benign odontogenic lesions. In Brazilian dental journal, 32, 74-82. doi:10.1590/0103-6440202104279. https://pubmed.ncbi.nlm.nih.gov/34787253/
5. Cheng, Shiliang, Li, Meng, Zheng, Wen, Zhuo, Jinhua, Zhang, Lu. 2024. ING3 inhibits the malignant progression of lung adenocarcinoma by negatively regulating ITGB4 expression to inactivate Src/FAK signaling. In Cellular signalling, 117, 111066. doi:10.1016/j.cellsig.2024.111066. https://pubmed.ncbi.nlm.nih.gov/38281617/
6. Mouche, Audrey, Archambeau, Jérôme, Ricordel, Charles, Grenon, Muriel, Pedeux, Rémy. 2019. ING3 is required for ATM signaling and DNA repair in response to DNA double strand breaks. In Cell death and differentiation, 26, 2344-2357. doi:10.1038/s41418-019-0305-x. https://pubmed.ncbi.nlm.nih.gov/30804473/
7. Nabbi, Arash, McClurg, Urszula L, Thalappilly, Subhash, Binda, Olivier, Riabowol, Karl T. 2017. ING3 promotes prostate cancer growth by activating the androgen receptor. In BMC medicine, 15, 103. doi:10.1186/s12916-017-0854-0. https://pubmed.ncbi.nlm.nih.gov/28511652/
8. Fink, Dieter, Yau, Tienyin, Nabbi, Arash, Riabowol, Karl, Rülicke, Thomas. 2019. Loss of Ing3 Expression Results in Growth Retardation and Embryonic Death. In Cancers, 12, . doi:10.3390/cancers12010080. https://pubmed.ncbi.nlm.nih.gov/31905726/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen