C57BL/6JCya-Ints3em1/Cya
Common Name:
Ints3-KO
Product ID:
S-KO-18289
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ints3-KO
Strain ID
KOCMP-229543-Ints3-B6J-VB
Gene Name
Product ID
S-KO-18289
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
3
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ints3em1/Cya mice (Catalog S-KO-18289) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029542
NCBI RefSeq
NM_145540
Target Region
Exon 4~5
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
INTS3, also known as Integrator subunit 3, is a crucial component of the integrator complex. It plays diverse essential functions, being involved in DNA damage repair pathways, homologous recombination-dependent repair of double-strand breaks (DSBs), and ataxia-telangiectasia mutated (ATM)-dependent signaling pathways. It also impacts RNA polymerase II (RNAPII) transcription termination and is related to RNA-binding protein functions, which are vital for maintaining genome stability and cell survival [2,4].
In acute myeloid leukaemia, IDH2 and SRSF2 double-mutant cells show aberrant splicing and reduced expression of INTS3, along with increased RNAPII stalling. Aberrant INTS3 splicing, in concert with mutant IDH2 and dependent on mutant SRSF2, contributes to leukaemogenesis [1].
In colorectal cancer, deletion/depletion of INTS3 triggers apoptosis, and in vitro experiments and RNA sequencing show it destabilizes pro-apoptotic gene transcripts, supporting CRC cell survival [3].
In conclusion, INTS3 has multifaceted functions in DNA damage repair, transcription termination, and cell survival-related processes. Its role in diseases like leukaemia and colorectal cancer, as revealed through functional studies, highlights its significance in understanding disease mechanisms. The study of INTS3 knockout or conditional knockout models in these disease areas can potentially provide more insights into its functions and offer new therapeutic strategies.
References:
1. Yoshimi, Akihide, Lin, Kuan-Ting, Wiseman, Daniel H, Krainer, Adrian R, Abdel-Wahab, Omar. 2019. Coordinated alterations in RNA splicing and epigenetic regulation drive leukaemogenesis. In Nature, 574, 273-277. doi:10.1038/s41586-019-1618-0. https://pubmed.ncbi.nlm.nih.gov/31578525/
2. Li, Jian, Ma, Xinli, Banerjee, Surajit, Bode, Ann M, Dong, Zigang. 2020. Structural basis for multifunctional roles of human Ints3 C-terminal domain. In The Journal of biological chemistry, 296, 100112. doi:10.1074/jbc.RA120.016393. https://pubmed.ncbi.nlm.nih.gov/33434574/
3. Wang, Zhiwei, Zhang, Cheng, Guo, Jing, Zhu, Pingping, He, Qiankun. 2024. CRISPR-Cas9 screening identifies INTS3 as an anti-apoptotic RNA-binding protein and therapeutic target for colorectal cancer. In iScience, 27, 109676. doi:10.1016/j.isci.2024.109676. https://pubmed.ncbi.nlm.nih.gov/38665208/
4. Fianu, Isaac, Ochmann, Moritz, Walshe, James L, Urlaub, Henning, Cramer, Patrick. 2024. Structural basis of Integrator-dependent RNA polymerase II termination. In Nature, 629, 219-227. doi:10.1038/s41586-024-07269-4. https://pubmed.ncbi.nlm.nih.gov/38570683/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen