Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Mbipem1/Cya
Common Name:
Mbip-KO
Product ID:
S-KO-18291
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Mbip-KO
Strain ID
KOCMP-217588-Mbip-B6J-VB
Gene Name
Mbip
Product ID
S-KO-18291
Gene Alias
4933408E06Rik
Background
C57BL/6JCya
NCBI ID
217588
Modification
Conventional knockout
Chromosome
12
Phenotype
MGI:1918320
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mbipem1/Cya mice (Catalog S-KO-18291) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000021416
NCBI RefSeq
NM_145442.2
Target Region
Exon 2~4
Size of Effective Region
~3.6 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Mbip, short for MAP3K12 binding inhibitory protein, is a component of the Ada2A containing complex (ATAC) and has been identified as a susceptibility gene in several cancers. It interacts with MAPK upstream kinase (MUK)/dual leucine zipper-bearing kinase/leucine-zipper protein kinase (MUK/DLK/ZPK) through its two tandemly orientated leucine-zipper-like motifs, and inhibits MUK/DLK/ZPK's activity to induce JNK/SAPK activation, thus regulating the MAPK pathway [4].

In esophageal squamous cell carcinoma (ESCC), Mbip expression is higher in cancer tissue than normal tissue. High Mbip expression is associated with deeper invasion and worse prognosis. Overexpression of Mbip promotes ESCC cell migration and invasion both in vitro and in vivo, while knockdown has the opposite effect. Mbip promotes epithelial-mesenchymal transition (EMT) via phosphorylation of JNK/p38 [1].

In non-small cell lung cancer (NSCLC), Mbip expression enhances cellular proliferation, migration, invasion in vitro and metastasis in vivo by mediating JNK pathway activation and inducing matrix metalloproteinases (MMPs) expression [2]. Also, certain SNPs in the Mbip gene are associated with an increased risk of breast cancer, and these SNPs may alter Mbip's role in the inactivation of the mitogen-activated protein kinase (MAPK) pathway [3,5].

In conclusion, Mbip plays a significant role in cancer metastasis and prognosis, especially in ESCC, NSCLC, and breast cancer, by regulating key signaling pathways. Studies on Mbip, including those using gene-knockout or knockdown models (implied by the functional changes upon knockdown), have enhanced our understanding of its function in cancer-related biological processes, providing potential biomarker and therapeutic target candidates for these cancers.

References:
1. Ma, Yanchun, Hua, Yuyan, Yin, XiaoJie, Yang, Jian, Zhang, Ling. 2024. MBIP promotes ESCC metastasis by activating MAPK pathway. In Cellular signalling, 115, 111040. doi:10.1016/j.cellsig.2024.111040. https://pubmed.ncbi.nlm.nih.gov/38199596/
2. Ochieng, Joshua Kapere, Kundu, Samrat T, Bajaj, Rakhee, Fradette, Jared J, Gibbons, Don L. 2020. MBIP (MAP3K12 binding inhibitory protein) drives NSCLC metastasis by JNK-dependent activation of MMPs. In Oncogene, 39, 6719-6732. doi:10.1038/s41388-020-01463-0. https://pubmed.ncbi.nlm.nih.gov/32963352/
3. Shareefi, Ghadah, Turkistani, Alaa Nabil, Alsayyah, Ahmed, Abdel Hadi, Maha, Alkharsah, Khaled R. 2020. Pathway-Affecting Single Nucleotide Polymorphisms (SNPs) in RPS6KA1 and MBIP Genes are Associated with Breast Cancer Risk. In Asian Pacific journal of cancer prevention : APJCP, 21, 2163-2168. doi:10.31557/APJCP.2020.21.7.2163. https://pubmed.ncbi.nlm.nih.gov/32711446/
4. Fukuyama, K, Yoshida, M, Yamashita, A, Hirai, S, Ohno, S. . MAPK upstream kinase (MUK)-binding inhibitory protein, a negative regulator of MUK/dual leucine zipper-bearing kinase/leucine zipper protein kinase. In The Journal of biological chemistry, 275, 21247-54. doi:. https://pubmed.ncbi.nlm.nih.gov/10801814/
5. Lee, Young Ho, Kim, Jae-Hoon, Song, Gwan Gyu. 2014. Genome-wide pathway analysis of breast cancer. In Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 35, 7699-705. doi:10.1007/s13277-014-2027-5. https://pubmed.ncbi.nlm.nih.gov/24805830/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest