C57BL/6JCya-Mbipem1/Cya
Common Name:
Mbip-KO
Product ID:
S-KO-18291
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Mbip-KO
Strain ID
KOCMP-217588-Mbip-B6J-VB
Gene Name
Product ID
S-KO-18291
Gene Alias
4933408E06Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mbipem1/Cya mice (Catalog S-KO-18291) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000021416
NCBI RefSeq
NM_145442.2
Target Region
Exon 2~4
Size of Effective Region
~3.6 kb
Detailed Document
Overview of Gene Research
Mbip, short for MAP3K12 binding inhibitory protein, is a component of the Ada2A containing complex (ATAC) and has been identified as a susceptibility gene in several cancers. It interacts with MAPK upstream kinase (MUK)/dual leucine zipper-bearing kinase/leucine-zipper protein kinase (MUK/DLK/ZPK) through its two tandemly orientated leucine-zipper-like motifs, and inhibits MUK/DLK/ZPK's activity to induce JNK/SAPK activation, thus regulating the MAPK pathway [4].
In esophageal squamous cell carcinoma (ESCC), Mbip expression is higher in cancer tissue than normal tissue. High Mbip expression is associated with deeper invasion and worse prognosis. Overexpression of Mbip promotes ESCC cell migration and invasion both in vitro and in vivo, while knockdown has the opposite effect. Mbip promotes epithelial-mesenchymal transition (EMT) via phosphorylation of JNK/p38 [1].
In non-small cell lung cancer (NSCLC), Mbip expression enhances cellular proliferation, migration, invasion in vitro and metastasis in vivo by mediating JNK pathway activation and inducing matrix metalloproteinases (MMPs) expression [2]. Also, certain SNPs in the Mbip gene are associated with an increased risk of breast cancer, and these SNPs may alter Mbip's role in the inactivation of the mitogen-activated protein kinase (MAPK) pathway [3,5].
In conclusion, Mbip plays a significant role in cancer metastasis and prognosis, especially in ESCC, NSCLC, and breast cancer, by regulating key signaling pathways. Studies on Mbip, including those using gene-knockout or knockdown models (implied by the functional changes upon knockdown), have enhanced our understanding of its function in cancer-related biological processes, providing potential biomarker and therapeutic target candidates for these cancers.
References:
1. Ma, Yanchun, Hua, Yuyan, Yin, XiaoJie, Yang, Jian, Zhang, Ling. 2024. MBIP promotes ESCC metastasis by activating MAPK pathway. In Cellular signalling, 115, 111040. doi:10.1016/j.cellsig.2024.111040. https://pubmed.ncbi.nlm.nih.gov/38199596/
2. Ochieng, Joshua Kapere, Kundu, Samrat T, Bajaj, Rakhee, Fradette, Jared J, Gibbons, Don L. 2020. MBIP (MAP3K12 binding inhibitory protein) drives NSCLC metastasis by JNK-dependent activation of MMPs. In Oncogene, 39, 6719-6732. doi:10.1038/s41388-020-01463-0. https://pubmed.ncbi.nlm.nih.gov/32963352/
3. Shareefi, Ghadah, Turkistani, Alaa Nabil, Alsayyah, Ahmed, Abdel Hadi, Maha, Alkharsah, Khaled R. 2020. Pathway-Affecting Single Nucleotide Polymorphisms (SNPs) in RPS6KA1 and MBIP Genes are Associated with Breast Cancer Risk. In Asian Pacific journal of cancer prevention : APJCP, 21, 2163-2168. doi:10.31557/APJCP.2020.21.7.2163. https://pubmed.ncbi.nlm.nih.gov/32711446/
4. Fukuyama, K, Yoshida, M, Yamashita, A, Hirai, S, Ohno, S. . MAPK upstream kinase (MUK)-binding inhibitory protein, a negative regulator of MUK/dual leucine zipper-bearing kinase/leucine zipper protein kinase. In The Journal of biological chemistry, 275, 21247-54. doi:. https://pubmed.ncbi.nlm.nih.gov/10801814/
5. Lee, Young Ho, Kim, Jae-Hoon, Song, Gwan Gyu. 2014. Genome-wide pathway analysis of breast cancer. In Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 35, 7699-705. doi:10.1007/s13277-014-2027-5. https://pubmed.ncbi.nlm.nih.gov/24805830/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen