C57BL/6JCya-Uxs1em1/Cya
Common Name:
Uxs1-KO
Product ID:
S-KO-18312
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Uxs1-KO
Strain ID
KOCMP-67883-Uxs1-B6J-VA
Gene Name
Product ID
S-KO-18312
Gene Alias
1600025I13Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Uxs1em1/Cya mice (Catalog S-KO-18312) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000126008
NCBI RefSeq
NM_026430.4
Target Region
Exon 2~4
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
Uxs1, or UDP-glucuronate decarboxylase 1, is a Golgi enzyme that converts UDP-glucuronic acid (UDPGA) to UDP-xylose. This conversion is crucial in the sugar nucleotide biosynthetic pathway, and Uxs1 also limits UDPGA production through negative feedback on UGDH, the enzyme immediately upstream of it [2].
In a study on choroidal neovascularization (CNV), a novel circRNA, circRNA Uxs1, was identified. It was upregulated in CNV patient specimens and CNV mouse models. Knockdown of circRNA Uxs1 interrupted endothelial cell tube formation, migration, and proliferation in vitro, and alleviated neovascularization in vivo by sponging miR-335-5p, which activated the mTOR/p70 S6k pathway via upregulating PGF expression [1].
In zebrafish, a mutation in uxs1 led to a lack of proteoglycan-rich extracellular matrix in craniofacial cartilages, defective chondrocyte organization, and disrupted gene expression related to skeletal development, indicating its essential role in skeletal extracellular matrix production and organization [3].
In cryptococci, uxs1 mutants accumulated UDP-glucuronic acid, down-regulated the expression of permease FCY2, and reduced cellular uptake of 5-fluorocytosine, contributing to drug resistance [4].
In summary, Uxs1 is essential for processes such as sugar nucleotide metabolism, CNV regulation, skeletal development, and drug resistance in fungi. Studies using gene-knockdown models in mice and zebrafish, as well as mutant models in fungi, have revealed its role in these biological processes and disease-related conditions, providing insights into potential therapeutic targets for CNV and understanding the mechanisms of skeletal development and drug resistance [1,3,4].
References:
1. Wu, Jiali, Chen, Jieqiong, Hu, Jing, Wang, Fenghua, Sun, Xiaodong. 2023. CircRNA Uxs1/miR-335-5p/PGF axis regulates choroidal neovascularization via the mTOR/p70 S6k pathway. In Translational research : the journal of laboratory and clinical medicine, 256, 41-55. doi:10.1016/j.trsl.2023.01.003. https://pubmed.ncbi.nlm.nih.gov/36690073/
2. Doshi, Mihir B, Lee, Namgyu, Tseyang, Tenzin, Spinelli, Jessica B, Kim, Dohoon. 2023. Disruption of sugar nucleotide clearance is a therapeutic vulnerability of cancer cells. In Nature, 623, 625-632. doi:10.1038/s41586-023-06676-3. https://pubmed.ncbi.nlm.nih.gov/37880368/
3. Eames, B Frank, Singer, Amy, Smith, Gabriel A, Raible, David W, Postlethwait, John H. 2010. UDP xylose synthase 1 is required for morphogenesis and histogenesis of the craniofacial skeleton. In Developmental biology, 341, 400-15. doi:10.1016/j.ydbio.2010.02.035. https://pubmed.ncbi.nlm.nih.gov/20226781/
4. Chang, Yun C, Lamichhane, Ami Khanal, Cai, Hongyi, Bennett, John E, Kwon-Chung, Kyung J. 2021. Moderate levels of 5-fluorocytosine cause the emergence of high frequency resistance in cryptococci. In Nature communications, 12, 3418. doi:10.1038/s41467-021-23745-1. https://pubmed.ncbi.nlm.nih.gov/34103502/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen