C57BL/6JCya-Mov10l1em1/Cya
Common Name:
Mov10l1-KO
Product ID:
S-KO-18336
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mov10l1-KO
Strain ID
KOCMP-83456-Mov10l1-B6J-VA
Gene Name
Product ID
S-KO-18336
Gene Alias
CHAMP; Csm
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mov10l1em1/Cya mice (Catalog S-KO-18336) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000015509
NCBI RefSeq
NM_031260
Target Region
Exon 3~13
Size of Effective Region
~20.2 kb
Detailed Document
Overview of Gene Research
MOV10L1, a germ cell-specific RNA helicase, is crucial for Piwi-interacting RNAs (piRNAs) biogenesis. PiRNAs are non-coding small RNAs that maintain genome integrity by silencing retrotransposons in germline cells. MOV10L1 is involved in both primary piRNA processing and the 'ping-pong' cycle during secondary piRNA processing [1].
In Mov10l1 knockout (KO) mouse models, primary spermatocytes show activation of LTR and LINE-1 retrotransposons, followed by cell death, leading to male infertility and a complete block of spermatogenesis at early prophase of meiosis I [2]. The genetic disruption of the MOV10L1 RNA helicase domain in mice renders MILI and MIWI2 devoid of piRNAs, causing loss of DNA methylation and subsequent derepression of retrotransposons in germ cells [4]. A point mutation that abolishes the 5'-to-3' RNA-unwinding activity of MOV10L1 in vivo causes a failure in primary piRNA biogenesis [5]. The yama mutation in the MOV10L1 N-terminal region, which results in a single amino acid substitution V229E, causes meiotic arrest, de-repression of transposable elements, and male sterility due to defects in pre-pachytene piRNA biogenesis [3].
In conclusion, MOV10L1 is essential for male fertility as it plays a key role in piRNA-directed retrotransposon silencing in male germ cells. The study of Mov10l1 KO mouse models has provided insights into the mechanism of spermatogenesis and the role of piRNAs in maintaining genome integrity in male germ cells [2,3,4,5].
References:
1. Zhu, Xiaobin, Zhi, Erlei, Li, Zheng. 2015. MOV10L1 in piRNA processing and gene silencing of retrotransposons during spermatogenesis. In Reproduction (Cambridge, England), 149, R229-35. doi:10.1530/REP-14-0569. https://pubmed.ncbi.nlm.nih.gov/25667429/
2. Frost, Robert J A, Hamra, F Kent, Richardson, James A, Bassel-Duby, Rhonda, Olson, Eric N. 2010. MOV10L1 is necessary for protection of spermatocytes against retrotransposons by Piwi-interacting RNAs. In Proceedings of the National Academy of Sciences of the United States of America, 107, 11847-52. doi:10.1073/pnas.1007158107. https://pubmed.ncbi.nlm.nih.gov/20547853/
3. Guan, Yongjuan, Keeney, Scott, Jain, Devanshi, Wang, P Jeremy. 2021. yama, a mutant allele of Mov10l1, disrupts retrotransposon silencing and piRNA biogenesis. In PLoS genetics, 17, e1009265. doi:10.1371/journal.pgen.1009265. https://pubmed.ncbi.nlm.nih.gov/33635934/
4. Zheng, Ke, Xiol, Jordi, Reuter, Michael, Pillai, Ramesh S, Wang, Peijing Jeremy. 2010. Mouse MOV10L1 associates with Piwi proteins and is an essential component of the Piwi-interacting RNA (piRNA) pathway. In Proceedings of the National Academy of Sciences of the United States of America, 107, 11841-6. doi:10.1073/pnas.1003953107. https://pubmed.ncbi.nlm.nih.gov/20534472/
5. Vourekas, Anastassios, Zheng, Ke, Fu, Qi, Mourelatos, Zissimos, Wang, P Jeremy. 2015. The RNA helicase MOV10L1 binds piRNA precursors to initiate piRNA processing. In Genes & development, 29, 617-29. doi:10.1101/gad.254631.114. https://pubmed.ncbi.nlm.nih.gov/25762440/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen