C57BL/6JCya-Fkbp1aem1/Cya
Common Name:
Fkbp1a-KO
Product ID:
S-KO-18354
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Fkbp1a-KO
Strain ID
KOCMP-14225-Fkbp1a-B6J-VA
Gene Name
Product ID
S-KO-18354
Gene Alias
FKBP12; Fkbp; Fkbp1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fkbp1aem1/Cya mice (Catalog S-KO-18354) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000044011
NCBI RefSeq
NM_008019
Target Region
Exon 3~4
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Fkbp1a, also known as FKBP12, is a ubiquitously expressed cis-trans peptidyl-prolyl isomerase. It is involved in multiple biological processes. It has been linked to pathways such as the apoptosis pathway and mTOR signaling pathway, and is critical for various tumors and cancers [1,2,3]. Genetic models, especially gene knockout (KO) models, are valuable for studying its functions.
In a KO mouse model, Fkbp1a-deficient mice develop ventricular hypertrabeculation and non-compaction. Endothelial cell-restricted ablation of Fkbp1a recapitulated this defect, suggesting Fkbp1a within the developing endocardia is important in regulating ventricular trabeculation and compaction. Fkbp1a was found to be a negative modulator of activated Notch1, with its ablation leading to upregulation of activated Notch1 (N1ICD) [4]. In glioblastoma cell lines, upregulated Fkbp1a promoted cell death, inhibiting glioblastoma growth via the apoptosis pathway [1].
In conclusion, Fkbp1a plays essential roles in processes like ventricular wall formation and tumor-related cell growth regulation. Gene knockout mouse models have revealed its functions in these specific biological and disease-related areas, contributing to a better understanding of related physiological and pathological mechanisms.
References:
1. Cai, Shaoyi, Chen, Zhiyou, Tang, Heng, Tao, Liang, Wang, Qin. 2022. Upregulated FKBP1A Suppresses Glioblastoma Cell Growth via Apoptosis Pathway. In International journal of molecular sciences, 23, . doi:10.3390/ijms232314935. https://pubmed.ncbi.nlm.nih.gov/36499275/
2. Bailus, Barbara J, Scheeler, Stephen M, Simons, Jesse, Ehrlich, Michelle E, Ellerby, Lisa M. 2021. Modulating FKBP5/FKBP51 and autophagy lowers HTT (huntingtin) levels. In Autophagy, 17, 4119-4140. doi:10.1080/15548627.2021.1904489. https://pubmed.ncbi.nlm.nih.gov/34024231/
3. Li, Zhongguang, Cui, Ying, Duan, Qinchun, Liu, Yingli, Xu, Xuehong. 2022. The Prognostic Significance of FKBP1A and Its Related Immune Infiltration in Liver Hepatocellular Carcinoma. In International journal of molecular sciences, 23, . doi:10.3390/ijms232112797. https://pubmed.ncbi.nlm.nih.gov/36361587/
4. Chen, Hanying, Zhang, Wenjun, Sun, Xiaoxin, Towbin, Jeffrey A, Shou, Weinian. . Fkbp1a controls ventricular myocardium trabeculation and compaction by regulating endocardial Notch1 activity. In Development (Cambridge, England), 140, 1946-57. doi:10.1242/dev.089920. https://pubmed.ncbi.nlm.nih.gov/23571217/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen