C57BL/6JCya-Mir32em1/Cya
Common Name:
Mir32-KO
Product ID:
S-KO-18365
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Mir32-KO
Strain ID
KOCMP-723837-Mir32-B6J-VA
Gene Name
Product ID
S-KO-18365
Gene Alias
Mirn32; mir-32; mmu-mir-32
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mir32em1/Cya mice (Catalog S-KO-18365) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000083610
NCBI RefSeq
NR_029789
Target Region
Exon 1
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Mir32, a microRNA, is a key regulator of gene expression at the post-transcriptional level [2]. It is involved in multiple biological processes such as cell proliferation, apoptosis, oncogenesis, invasion, metastasis, and drug resistance [2]. Alterations in its expression have been associated with numerous diseases, including metabolic, cardiovascular disorders, and various cancers [2].
In the context of vascular smooth muscle cell (VSMC) calcification, miR32-5p promoted VSMC calcification by upregulating TNFα in the microenvironment. Bioinformatics analysis and experimental verification showed that miR32-5p could target PIKfyve, affecting the production of TNFα in microglia [1].
In the liver, miR-32-5p was found to induce hepatic steatosis and hyperlipidemia by triggering de novo lipogenesis. Hepatocyte-specific miR-32 knockout (miR-32-HKO) ameliorated hepatic steatosis and metabolic disorders in high-fat diet-fed mice, while hepatic miR-32 overexpression exacerbated these abnormalities [3].
In prostate cancer, transgenic overexpression of miR-32 in hiMYC mice increased tumor burden and led to a more aggressive tumor phenotype, and Pdk4 was identified as a tumor-associated miR-32 target [4].
In conclusion, Mir32 plays diverse and significant roles in various biological processes and disease conditions. Studies using gene knockout models, like miR-32-HKO in mice, have revealed its functions in diseases such as vascular calcification, hepatic steatosis, and prostate cancer. These findings contribute to a better understanding of the underlying mechanisms of these diseases and may provide potential therapeutic targets.
References:
1. Cao, Jingsong, Chen, Ling, Zhong, Xiaoling, Zu, Xuyu, Liu, Jianghua. 2020. miR32-5p promoted vascular smooth muscle cell calcification by upregulating TNFα in the microenvironment. In BMC immunology, 21, 3. doi:10.1186/s12865-019-0324-x. https://pubmed.ncbi.nlm.nih.gov/31952480/
2. Zeng, Z L, Zhu, Qingyun, Zhao, Zhibo, Zu, Xuyu, Liu, Jianghua. 2021. Magic and mystery of microRNA-32. In Journal of cellular and molecular medicine, 25, 8588-8601. doi:10.1111/jcmm.16861. https://pubmed.ncbi.nlm.nih.gov/34405957/
3. Wang, Ya-Di, Wu, Liang-Liang, Mai, Yun-Ni, Liu, Jiang-Hua, Xiao, Xin-Hua. 2023. miR-32-5p induces hepatic steatosis and hyperlipidemia by triggering de novo lipogenesis. In Metabolism: clinical and experimental, 146, 155660. doi:10.1016/j.metabol.2023.155660. https://pubmed.ncbi.nlm.nih.gov/37451670/
4. Scaravilli, Mauro, Koivukoski, Sonja, Gillen, Andrew, Visakorpi, Tapio, Latonen, Leena. 2022. miR-32 promotes MYC-driven prostate cancer. In Oncogenesis, 11, 11. doi:10.1038/s41389-022-00385-8. https://pubmed.ncbi.nlm.nih.gov/35228520/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen