C57BL/6JCya-Tmed4em1/Cya
Common Name
Tmed4-KO
Product ID
S-KO-18366
Backgroud
C57BL/6JCya
Strain ID
KOCMP-103694-Tmed4-B6J-VB
When using this mouse strain in a publication, please cite “Tmed4-KO Mouse (Catalog S-KO-18366) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Tmed4-KO
Strain ID
KOCMP-103694-Tmed4-B6J-VB
Gene Name
Product ID
S-KO-18366
Gene Alias
1110014L17Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 11
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000004508
NCBI RefSeq
NM_134020
Target Region
Exon 1~4
Size of Effective Region
~3.5 kb
Overview of Gene Research
Tmed4, also known as transmembrane p24 trafficking protein 4, is an endoplasmic reticulum stress (ERS)-related protein. It is involved in maintaining regulatory T cell (Treg) stability and function, potentially through protein vesicular trafficking and antioxidant response pathways [1,2]. Tmed4 plays a crucial role in biological processes related to immune regulation and may impact various disease conditions.
In Treg-specific knockout (Tmed4ΔTreg) mice, loss of Tmed4 led to more Tregs with impaired Foxp3 stability, Treg signatures, and suppressive activity. This caused T cell hyperactivation, an exacerbated inflammatory phenotype, and boosted antitumor immunity. Mechanistically, Tmed4 deficiency caused defects in ERS and the NRF2-related antioxidant response, resulting in excessive ROS that reduced Treg function in an IRE1α/XBP1 axis-dependent manner. These abnormalities could be rescued by a ROS scavenger, NRF2 inducer, or forced expression of IRE1α. Also, TMED4 suppressed IRE1α proteosome degradation via the ER-associated degradation (ERAD) system [1].
In conclusion, Tmed4 is essential for maintaining the stability of Tregs and their suppressive function through the IRE1α-dependent ROS and NRF2-related antioxidant response. The Tmed4ΔTreg mouse model has revealed its significance in tumor and autoimmune disease conditions, contributing to our understanding of immune-related disease mechanisms [1].
References：
1. Jiang, Zhenyan, Wang, Huizi, Wang, Xiaoxia, Li, Bin, Wu, Xuefeng. 2024. TMED4 facilitates regulatory T cell suppressive function via ROS homeostasis in tumor and autoimmune mouse models. In The Journal of clinical investigation, 135, . doi:10.1172/JCI179874. https://pubmed.ncbi.nlm.nih.gov/39480507/
2. Guo, Xin, Zhou, Wei, Jin, Jinmei, Zhang, Lijun, Luan, Xin. 2024. Integrative Multi-Omics Analysis Identifies Transmembrane p24 Trafficking Protein 1 (TMED1) as a Potential Prognostic Marker in Colorectal Cancer. In Biology, 13, . doi:10.3390/biology13020083. https://pubmed.ncbi.nlm.nih.gov/38392302/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.