C57BL/6JCya-F3em1/Cya
Common Name:
F3-KO
Product ID:
S-KO-18367
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
F3-KO
Strain ID
KOCMP-14066-F3-B6J-VA
Gene Name
Product ID
S-KO-18367
Gene Alias
CD142; Cf-3; Cf3; TF
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-F3em1/Cya mice (Catalog S-KO-18367) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029771
NCBI RefSeq
NM_010171
Target Region
Exon 2
Size of Effective Region
~0.1 kb
Detailed Document
Overview of Gene Research
F3, also known as Contactin, is a neuronal cell adhesion molecule belonging to the immunoglobulin superfamily. It has a modular structure with multiple binding sites, displaying heterophilic binding activities. F3 is involved in axogenesis, myelination, neuron-glial contacts, and organization of axonal subdomains at the node of Ranvier. It interacts with L1 family of Ig-CAMs, extracellular matrix components, and RPTPbeta/phosphacan, influencing axonal growth, fasciculation, and sensory axon guidance [1].
F3-deficient mice display severe ataxia due to defects in axonal and dendritic projections in the cerebellum, as well as nerve influx conduction defects from disrupted axo-glial contacts at paranodes [1]. In the hippocampus of mice, both chronic restraint stress and chronic social defeat stress decrease the expression of F3/Contactin. Over-expression of hippocampal F3/Contactin can prevent chronic stress-induced depressive-like behaviors, reverse the dysfunction in BDNF-CREB signaling and neurogenesis, while knockdown of it abolishes the protecting effects of vortioxetine against chronic stress [2].
In conclusion, F3 is crucial for neural development, playing a role in axonal and dendritic projections, myelination, and axo-glial contacts. In addition, it is implicated in depression, as its expression in the hippocampus is associated with stress-induced depressive-like behaviors and the action of antidepressants. The study of F3 using mouse models helps to understand its biological functions and its potential as a therapeutic target for depression.
References:
1. Falk, Julien, Bonnon, Carine, Girault, Jean-Antoine, Faivre-Sarrailh, Catherine. . F3/contactin, a neuronal cell adhesion molecule implicated in axogenesis and myelination. In Biology of the cell, 94, 327-34. doi:. https://pubmed.ncbi.nlm.nih.gov/12500940/
2. Chen, Yan-Mei, Fan, Hua, Huang, Jie, Jiang, Bo, Liu, Jian-Feng. 2022. Hippocampal F3/Contactin plays a role in chronic stress-induced depressive-like effects and the antidepressant actions of vortioxetine in mice. In Biochemical pharmacology, 202, 115097. doi:10.1016/j.bcp.2022.115097. https://pubmed.ncbi.nlm.nih.gov/35609645/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen