C57BL/6JCya-Mmp10em1/Cya
Common Name:
Mmp10-KO
Product ID:
S-KO-18384
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mmp10-KO
Strain ID
KOCMP-17384-Mmp10-B6J-VB
Gene Name
Product ID
S-KO-18384
Gene Alias
MMP-10; SL-2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mmp10em1/Cya mice (Catalog S-KO-18384) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034488
NCBI RefSeq
NM_019471
Target Region
Exon 2~4
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Mmp10, also known as Stromelysin-2, is a matrix metalloproteinase involved in various immune-related and disease-associated processes. It plays a role in controlling macrophage activation and is associated with pathways such as the PPARγ-STAT3 signaling pathway. MMP10 is crucial for maintaining the balance of immune responses and has overall biological importance in tissue repair, inflammation regulation, and disease development [1,2,9]. Genetic models, especially knockout (KO) mouse models, have been valuable in studying its functions.
In non-alcoholic steatohepatitis (NASH), MMP10-OE mice showed diminished hepatic steatosis and inflammation, while MMP10-KO mice had an imbalanced M1/M2 macrophage ratio and aggravated NASH progression. The PPARγ-MMP10-STAT3 axis promoted M2 macrophage polarization, alleviating NASH [1]. In the tolerance to TLR7-induced skin inflammation, Mmp10-/-mice failed to develop immune hypo-responsiveness, indicating MMP10's role in TLR7-mediated tolerance [2]. In ischemic stroke, the rs17860949 polymorphism in MMP10 was associated with a reduced risk, especially in certain subgroups [3]. In tongue cancer, MMP10 overexpression promoted lymph node metastasis, while its knockdown suppressed it, and miR-944 negatively regulated MMP10 [4]. In a diabetic murine model of ischemic stroke, MMP10 treatment was more effective than tPA in reducing infarct size and neurodegeneration [5]. In tracheal fibrosis, SOX9 promoted ECM deposition through up-regulating MMP10, and SOX9 knockdown ameliorated tracheal fibrosis [6]. In colitis and colon tumorigenesis, miR-148/152 family deficiency elevated MMP10, disrupting the intestinal barrier and activating NF-κB signaling [7]. In steroid-induced osteonecrosis of the femoral head, the rs470154 polymorphism in MMP10 was associated with an increased risk [8]. In acute infection, Mmp10-/-mice had a more severe pro-inflammatory response, and adoptive transfer of wild-type macrophages normalized the morbidity, showing MMP10's role in moderating macrophage-mediated inflammation [9]. In rheumatoid arthritis, miR-496 negatively regulated MMP10, affecting the proliferation and apoptosis of fibroblast-like synoviocytes via the NF-κB signaling pathway [10].
In conclusion, MMP10 plays essential roles in multiple biological processes, especially in macrophage-mediated immune responses, inflammation regulation, and fibrosis. Studies using KO mouse models have revealed its significance in diseases like NASH, ischemic stroke, tongue cancer, colitis, and more, providing potential therapeutic targets for these diseases.
References:
1. Chang, Ling, Gao, Junda, Yu, Yeping, Zhou, Tao, Xu, Qihua. 2023. MMP10 alleviates non-alcoholic steatohepatitis by regulating macrophage M2 polarization. In International immunopharmacology, 124, 111045. doi:10.1016/j.intimp.2023.111045. https://pubmed.ncbi.nlm.nih.gov/37844469/
2. Rohani, Maryam G, Dimitrova, Elizabeth, Beppu, Andrew, Jefferies, Caroline A, Parks, William C. 2018. Macrophage MMP10 Regulates TLR7-Mediated Tolerance. In Frontiers in immunology, 9, 2817. doi:10.3389/fimmu.2018.02817. https://pubmed.ncbi.nlm.nih.gov/30564235/
3. Zhao, Yong, Zhang, Qi, Zhang, Xiaobo, Shi, Wenzhen, Tian, Ye. 2022. The roles of MMP8/MMP10 polymorphisms in ischemic stroke susceptibility. In Brain and behavior, 12, e2797. doi:10.1002/brb3.2797. https://pubmed.ncbi.nlm.nih.gov/36282475/
4. Dharavath, Bhasker, Butle, Ashwin, Pal, Ankita, Nair, Sudhir, Dutt, Amit. 2023. Role of miR-944/MMP10/AXL- axis in lymph node metastasis in tongue cancer. In Communications biology, 6, 57. doi:10.1038/s42003-023-04437-6. https://pubmed.ncbi.nlm.nih.gov/36650344/
5. Navarro-Oviedo, Manuel, Roncal, Carmen, Salicio, Agustina, Muñoz, Roberto, Orbe, Josune. 2018. MMP10 Promotes Efficient Thrombolysis After Ischemic Stroke in Mice with Induced Diabetes. In Translational stroke research, 10, 389-401. doi:10.1007/s12975-018-0652-9. https://pubmed.ncbi.nlm.nih.gov/30051168/
6. Gu, Lei, Li, Anmao, He, Chunyan, Li, Yishi, Guo, Shuliang. 2023. Profibrotic role of the SOX9-MMP10-ECM biosynthesis axis in the tracheal fibrosis after injury and repair. In Genes & diseases, 11, 101040. doi:10.1016/j.gendis.2023.06.012. https://pubmed.ncbi.nlm.nih.gov/38993791/
7. Tang, Kaiwen, Wu, Zhonghua, Sun, Mingwei, Song, Yongxi, Wang, Zhenning. 2022. Elevated MMP10/13 mediated barrier disruption and NF-κB activation aggravate colitis and colon tumorigenesis in both individual or full miR-148/152 family knockout mice. In Cancer letters, 529, 53-69. doi:10.1016/j.canlet.2021.12.033. https://pubmed.ncbi.nlm.nih.gov/34979166/
8. Tian, Ye, An, Feimeng, Wang, Jiaqi, Wang, Jianzhong, Wang, Guoqiang. 2019. MMP2 and MMP10 Polymorphisms Are Related to Steroid-Induced Osteonecrosis of the Femoral Head among Chinese Han Population. In BioMed research international, 2019, 8298193. doi:10.1155/2019/8298193. https://pubmed.ncbi.nlm.nih.gov/31192258/
9. McMahan, Ryan S, Birkland, Timothy P, Smigiel, Kate S, Gharib, Sina A, Parks, William C. 2016. Stromelysin-2 (MMP10) Moderates Inflammation by Controlling Macrophage Activation. In Journal of immunology (Baltimore, Md. : 1950), 197, 899-909. doi:10.4049/jimmunol.1600502. https://pubmed.ncbi.nlm.nih.gov/27316687/
10. Xing, Xue-Wu, Shi, Hong-Yu, Liu, Shen, Feng, Shi-Qing, Gong, Bao-Qi. 2021. miR-496/MMP10 Is Involved in the Proliferation of IL-1β-Induced Fibroblast-Like Synoviocytes Via Mediating the NF-κB Signaling Pathway. In Inflammation, 44, 1359-1369. doi:10.1007/s10753-021-01421-2. https://pubmed.ncbi.nlm.nih.gov/33548006/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen