C57BL/6JCya-Wdhd1em1/Cya
Common Name:
Wdhd1-KO
Product ID:
S-KO-18386
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Wdhd1-KO
Strain ID
KOCMP-218973-Wdhd1-B6J-VB
Gene Name
Product ID
S-KO-18386
Gene Alias
A630092E18; AND-1; D630024B06Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Wdhd1em1/Cya mice (Catalog S-KO-18386) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000187531
NCBI RefSeq
NM_001360532
Target Region
Exon 3
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Wdhd1, short for WD repeat and HMG-box DNA binding protein 1, is the mouse ortholog of budding yeast Chromosome Transmission Fidelity 4 (CTF4). It integrates the MCM2-7 helicase and DNA polymerase α/primase complex to initiate DNA replication, playing a crucial role in DNA synthesis [1]. It is also a downstream target of the PI3K/AKT pathway, and its phosphorylation by AKT can induce DNA replication, which may be related to the occurrence of esophageal cancer [2].
In mice, Wdhd1 null allele generated through Nuclease technology-mediated genome editing showed that while Wdhd1 heterozygous mutant mice were normal and fertile, homozygous null mutant embryos had reduced cell proliferation by the gastrulation stage, hampering their growth and viability, indicating its major role in cell proliferation during embryogenesis [1]. In lung adenocarcinoma, knocking out Wdhd1 increased the sensitivity of cisplatin-resistant cells to cisplatin, and WDHD1 overexpression negatively correlated with the overall survival of patients. WDHD1 promoted the ubiquitination of MAPRE2 to lead to cisplatin resistance [3]. In PTEN-inactive triple-negative breast cancer, knockdown of WDHD1 significantly inhibited cell viability, suggesting it could be a potential biomarker or therapeutic target [4].
In conclusion, Wdhd1 is essential for early mouse embryogenesis, especially in cell proliferation. Its dysregulation is associated with multiple cancers such as esophageal cancer, lung adenocarcinoma, and triple-negative breast cancer. Studies using gene knockout mouse models have provided valuable insights into its functions and potential as a therapeutic target in these disease areas.
References:
1. Fan, Hsiang-Hsuan, Lee, Kuo-Hong, Chen, You-Tzung, Lin, Shu-Wha, Yu, I-Shing. 2021. Wdhd1 is essential for early mouse embryogenesis. In Biochimica et biophysica acta. Molecular cell research, 1868, 119011. doi:10.1016/j.bbamcr.2021.119011. https://pubmed.ncbi.nlm.nih.gov/33716172/
2. Xian, Qingying, Zhu, Danxia. 2022. The Involvement of WDHD1 in the Occurrence of Esophageal Cancer as a Downstream Target of PI3K/AKT Pathway. In Journal of oncology, 2022, 5871188. doi:10.1155/2022/5871188. https://pubmed.ncbi.nlm.nih.gov/35422862/
3. Gong, Lian, Xiao, Mengqing, He, Dong, Lan, Hua, Cao, Ke. 2020. WDHD1 Leads to Cisplatin Resistance by Promoting MAPRE2 Ubiquitination in Lung Adenocarcinoma. In Frontiers in oncology, 10, 461. doi:10.3389/fonc.2020.00461. https://pubmed.ncbi.nlm.nih.gov/32426268/
4. Ertay, Ayse, Liu, Huiquan, Liu, Dian, Downward, Julian, Wang, Yihua. 2020. WDHD1 is essential for the survival of PTEN-inactive triple-negative breast cancer. In Cell death & disease, 11, 1001. doi:10.1038/s41419-020-03210-5. https://pubmed.ncbi.nlm.nih.gov/33221821/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen