C57BL/6JCya-Cdk8em1/Cya
Common Name:
Cdk8-KO
Product ID:
S-KO-18387
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cdk8-KO
Strain ID
KOCMP-264064-Cdk8-B6J-VA
Gene Name
Product ID
S-KO-18387
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cdk8em1/Cya mice (Catalog S-KO-18387) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031640
NCBI RefSeq
NM_153599
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Cdk8, cyclin-dependent kinase 8, is a key component in transcription regulation. It forms a kinase module or phosphorylates transcription factors, playing a momentous role in the Mediator complex. Cdk8 is involved in multiple biological processes, and its dysregulation is associated with various diseases, especially cancer [3].
In Drosophila, neuronal loss of Cdk8 severely reduces fly lifespan and causes bang sensitivity. Cdk8 promotes mitochondrial fission through Drp1 phosphorylation and can phenotypically suppress pink1 deficiency [1]. In T cells, inhibition of Cdk8/19 can convert antigen-specific effector/memory T cells into Foxp3-expressing Treg cells, suggesting its role in immune-related diseases [2]. Cdk8 inhibitors can also antagonize HIV-1 reactivation and promote provirus latency in T cells [4].
In summary, Cdk8 is crucial for transcription regulation and is involved in diverse biological processes. Studies in Drosophila and T cells, as well as the effects of its inhibitors on HIV-1, have revealed its significance in lifespan, mitochondrial function, immune-related diseases, and viral latency. Understanding Cdk8's functions may provide new therapeutic opportunities for these disease areas.
References:
1. Liao, Jenny Zhe, Chung, Hyung-Lok, Shih, Claire, Bellen, Hugo J, Verheyen, Esther M. 2024. Cdk8/CDK19 promotes mitochondrial fission through Drp1 phosphorylation and can phenotypically suppress pink1 deficiency in Drosophila. In Nature communications, 15, 3326. doi:10.1038/s41467-024-47623-8. https://pubmed.ncbi.nlm.nih.gov/38637532/
2. Akamatsu, Masahiko, Mikami, Norihisa, Ohkura, Naganari, Narumiya, Shuh, Sakaguchi, Shimon. . Conversion of antigen-specific effector/memory T cells into Foxp3-expressing Treg cells by inhibition of CDK8/19. In Science immunology, 4, . doi:10.1126/sciimmunol.aaw2707. https://pubmed.ncbi.nlm.nih.gov/31653719/
3. Wu, Dan, Zhang, Zhaoyan, Chen, Xing, Yan, Yaoyao, Liu, Xinhua. 2020. Angel or Devil ? - CDK8 as the new drug target. In European journal of medicinal chemistry, 213, 113043. doi:10.1016/j.ejmech.2020.113043. https://pubmed.ncbi.nlm.nih.gov/33257171/
4. Horvath, Riley M, Brumme, Zabrina L, Sadowski, Ivan. 2023. CDK8 inhibitors antagonize HIV-1 reactivation and promote provirus latency in T cells. In Journal of virology, 97, e0092323. doi:10.1128/jvi.00923-23. https://pubmed.ncbi.nlm.nih.gov/37671866/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen