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C57BL/6JCya-Rnf180em1/Cya
Common Name:
Rnf180-KO
Product ID:
S-KO-18393
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Rnf180-KO
Strain ID
KOCMP-71816-Rnf180-B6J-VB
Gene Name
Rnf180
Product ID
S-KO-18393
Gene Alias
3110001E11Rik; Rines
Background
C57BL/6JCya
NCBI ID
71816
Modification
Conventional knockout
Chromosome
13
Phenotype
MGI:1919066
Document
Click here to download >>
Application
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Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rnf180em1/Cya mice (Catalog S-KO-18393) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000224662
NCBI RefSeq
NM_001360891
Target Region
Exon 6
Size of Effective Region
~0.2 kb
Detailed Document
Click here to download >>
Overview of Gene Research
RNF180, an important member of the E3 ubiquitin ligase family, is considered a tumor suppressor gene. It is involved in multiple cellular processes such as cell development, proliferation, and apoptosis. In signaling pathways, RNF180 has been linked to regulating the STAT3 pathway via RhoC in gastric cancer cells, and it may also participate in other pathways related to tumorigenesis and inflammation [2].

In colorectal cancer, RNF180 overexpression inhibits cell viability and induces apoptosis, while its depletion enhances cell survival. It ubiquitinates WISP1, a critical downstream molecule, leading to WISP1 downregulation and suppression of tumor growth in patient-derived xenograft mouse models.

In gastric cancer, RNF180 can inhibit cell proliferation, invasion, and migration. It reduces STAT3 phosphorylation by promoting the ubiquitination and degradation of RhoC. Additionally, RNF180 can prevent excessive PCDH10 methylation by promoting ubiquitination of DNMT1, thus suppressing gastric cancer cell proliferation and metastasis.

In triple-negative breast cancer, RNF180 degrades RAD51 by ubiquitination, inhibiting cell growth, metastasis, and sensitizing cells to Gefitinib.

In ovarian cancer, RNF180 impairs the IPO4/SOX2 complex stability, inhibits SOX2-mediated malignancy, and suppresses the progression of ovarian cancer [1,2,3,4,5].

In conclusion, RNF180 plays a crucial role in suppressing tumor growth and development through various mechanisms, mainly by ubiquitinating key downstream molecules. Studies using different models, including patient-derived xenograft mouse models, have revealed its significance in cancers such as colorectal, gastric, triple-negative breast, and ovarian cancers. These findings provide potential therapeutic targets for these diseases.

References:
1. Wei, Feng, Ba, Sang, Jin, Mei, E, Fusheng, Long, Ziwen. 2021. RNF180 Inhibits Proliferation and Promotes Apoptosis of Colorectal Cancer Through Ubiquitination of WISP1. In Frontiers in cell and developmental biology, 8, 623455. doi:10.3389/fcell.2020.623455. https://pubmed.ncbi.nlm.nih.gov/33553163/
2. Wu, Zizhen, Liu, Huifang, Sun, Weilin, Liang, Han, Deng, Jingyu. 2020. RNF180 mediates STAT3 activity by regulating the expression of RhoC via the proteasomal pathway in gastric cancer cells. In Cell death & disease, 11, 881. doi:10.1038/s41419-020-03096-3. https://pubmed.ncbi.nlm.nih.gov/33082325/
3. Zhang, Nannan, Gao, Xiaoliang, Yuan, Qiangqiang, Nie, Yongzhan, Deng, Jingyu. 2023. E3 ubiquitin ligase RNF180 prevents excessive PCDH10 methylation to suppress the proliferation and metastasis of gastric cancer cells by promoting ubiquitination of DNMT1. In Clinical epigenetics, 15, 77. doi:10.1186/s13148-023-01492-y. https://pubmed.ncbi.nlm.nih.gov/37147733/
4. Liu, Pian, Zhou, Wei, Yang, Liu, Zhang, Chen. 2022. E3 ubiquitin ligase RNF180 reduces sensitivity of triple-negative breast cancer cells to Gefitinib by downregulating RAD51. In Chemico-biological interactions, 354, 109798. doi:10.1016/j.cbi.2022.109798. https://pubmed.ncbi.nlm.nih.gov/34998818/
5. Zhao, Haiyan, Bi, Fangfang, Li, Mengyuan, Diao, Yuhan, Zhang, Chen. 2023. E3 ubiquitin ligase RNF180 impairs IPO4/SOX2 complex stability and inhibits SOX2-mediated malignancy in ovarian cancer. In Cellular signalling, 113, 110961. doi:10.1016/j.cellsig.2023.110961. https://pubmed.ncbi.nlm.nih.gov/37923100/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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