C57BL/6JCya-Fbxo11em1/Cya
Common Name:
Fbxo11-KO
Product ID:
S-KO-18396
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Fbxo11-KO
Strain ID
KOCMP-225055-Fbxo11-B6J-VA
Gene Name
Product ID
S-KO-18396
Gene Alias
Jf
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fbxo11em1/Cya mice (Catalog S-KO-18396) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000005504
NCBI RefSeq
NM_001081034
Target Region
Exon 4
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Fbxo11, also known as VIT1 and PRMT9, is the substrate-recognition component of the Skp1-Cullin-1-F-box (SCF) E3 ubiquitin-ligase complex. It is involved in ubiquitin-dependent proteasomal degradation processes, which play crucial roles in various biological pathways such as immune regulation, bone development, lipid metabolism, and antiviral responses [1,2,3,4]. Genetic models, especially gene knockout (KO) and conditional knockout (CKO) mouse models, have been valuable in studying its functions.
In KO/CKO mouse models, osteoblastic-specific FBXO11 deficiency inhibits normal bone growth and osteogenic activity through Snail1 protein accumulation, demonstrating its role in bone development [2]. In immune-related studies, FBXO11 deficiency in human and mouse cells leads to increased levels of MHC-II and related genes, indicating its negative regulation of MHC-II through ubiquitination-mediated degradation of CIITA [1].
In conclusion, Fbxo11 is essential for multiple biological functions mainly through its role in the ubiquitination-mediated degradation of key proteins. Studies using KO/CKO mouse models have revealed its importance in bone development and immune regulation, which may provide insights into related disease mechanisms such as bone growth disorders and immune-related cancers [1,2].
References：
1. Kasuga, Yusuke, Ouda, Ryota, Watanabe, Masashi, Hatakeyama, Shigetsugu, Kobayashi, Koichi S. 2023. FBXO11 constitutes a major negative regulator of MHC class II through ubiquitin-dependent proteasomal degradation of CIITA. In Proceedings of the National Academy of Sciences of the United States of America, 120, e2218955120. doi:10.1073/pnas.2218955120. https://pubmed.ncbi.nlm.nih.gov/37279268/
2. Huang, Hong, Lu, Jianrong, Aukhil, Ikramuddin, Pirih, Flavia, Chang, Jia. 2023. FBXO11 regulates bone development. In Bone, 170, 116709. doi:10.1016/j.bone.2023.116709. https://pubmed.ncbi.nlm.nih.gov/36863499/
3. Zhang, Hao, Xia, Peng, Yang, Zhangshuo, Zhang, Zhonglin, Yuan, Yufeng. . Cullin-associated and neddylation-dissociated 1 regulate reprogramming of lipid metabolism through SKP1-Cullin-1-F-boxFBXO11 -mediated heterogeneous nuclear ribonucleoprotein A2/B1 ubiquitination and promote hepatocellular carcinoma. In Clinical and translational medicine, 13, e1443. doi:10.1002/ctm2.1443. https://pubmed.ncbi.nlm.nih.gov/37837399/
4. Gao, Long, Gao, Yufeng, Han, Kexing, Li, Jiabin, Qin, Frank Xiao-Feng. . FBXO11 amplifies type I interferon signaling to exert antiviral effects by facilitating the assemble of TRAF3-TBK1-IRF3 complex. In Journal of medical virology, 95, e28655. doi:10.1002/jmv.28655. https://pubmed.ncbi.nlm.nih.gov/36897010/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen