C57BL/6JCya-Lats1em1/Cya
Common Name:
Lats1-KO
Product ID:
S-KO-18413
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Lats1-KO
Strain ID
KOCMP-16798-Lats1-B6J-VA
Gene Name
Product ID
S-KO-18413
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lats1em1/Cya mice (Catalog S-KO-18413) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000165952
NCBI RefSeq
NM_010690
Target Region
Exon 5
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
Lats1, short for large tumor suppressor 1, is a key kinase in the Hippo signaling pathway. The Hippo pathway is evolutionarily conserved and crucial for regulating various biological processes such as cell growth, fate decision, organ size control, and regeneration [6]. Lats1, along with Lats2, phosphorylates and inhibits YAP and TAZ, two major effectors of the Hippo pathway, thus playing a significant role in tumor suppression [2].
In murine syngeneic tumor models, loss of Lats1/2 in tumor cells inhibits tumor growth, and this regression requires adaptive immune responses. Lats1/2-null tumor cells secrete nucleic-acid-rich extracellular vesicles, inducing a type I interferon response, which improves tumor immunogenicity [1]. In endometrial cancer, Lats1/2 loss promotes tumor immune evasion through down-regulating MHC-I expression, independent of the Hippo-YAP pathway. Lats1/2 directly interact with and phosphorylate STAT1 to enhance MHC-I transcriptional activation [3]. In breast cancer, knockout of METTL3 or YTHDF2, which regulate Lats1 mRNA through m6A methylation, increases Lats1 mRNA expression and suppresses tumorigenesis [4]. In ovarian cancer cells, deletion of S1PR1 increases Lats1/2 expression, promoting cell senescence, while silencing Lats1/2 suppresses senescence [5].
In conclusion, Lats1, as a core component of the Hippo pathway, plays a vital role in tumor-related biological processes. Gene knockout studies in mouse models have revealed its significance in tumor immunity, immune evasion, and cancer cell senescence, providing potential targets for cancer immunotherapy and chemotherapy.
References:
1. Moroishi, Toshiro, Hayashi, Tomoko, Pan, Wei-Wei, Carson, Dennis A, Guan, Kun-Liang. . The Hippo Pathway Kinases LATS1/2 Suppress Cancer Immunity. In Cell, 167, 1525-1539.e17. doi:10.1016/j.cell.2016.11.005. https://pubmed.ncbi.nlm.nih.gov/27912060/
2. Qi, Sixian, Zhu, Yuwen, Liu, Xincheng, Zhang, Lei, Yu, Fa-Xing. 2022. WWC proteins mediate LATS1/2 activation by Hippo kinases and imply a tumor suppression strategy. In Molecular cell, 82, 1850-1864.e7. doi:10.1016/j.molcel.2022.03.027. https://pubmed.ncbi.nlm.nih.gov/35429439/
3. Yang, Qianlan, Lv, Zehen, Wang, Mengfei, Gao, Kun, Wan, Xiaoping. 2024. LATS1/2 loss promote tumor immune evasion in endometrial cancer through downregulating MHC-I expression. In Journal of experimental & clinical cancer research : CR, 43, 54. doi:10.1186/s13046-024-02979-z. https://pubmed.ncbi.nlm.nih.gov/38383447/
4. Xu, Youqin, Song, Mu, Hong, Ziyang, Liu, Minfeng, Zuo, Qiang. 2023. The N6-methyladenosine METTL3 regulates tumorigenesis and glycolysis by mediating m6A methylation of the tumor suppressor LATS1 in breast cancer. In Journal of experimental & clinical cancer research : CR, 42, 10. doi:10.1186/s13046-022-02581-1. https://pubmed.ncbi.nlm.nih.gov/36609396/
5. Tao, Yi-Ping, Zhu, Heng-Yan, Shi, Qian-Yuan, Cheng, Shu-Qun, Pan, Wei-Wei. 2023. S1PR1 regulates ovarian cancer cell senescence through the PDK1-LATS1/2-YAP pathway. In Oncogene, 42, 3491-3502. doi:10.1038/s41388-023-02853-w. https://pubmed.ncbi.nlm.nih.gov/37828220/
6. Ma, Shenghong, Meng, Zhipeng, Chen, Rui, Guan, Kun-Liang. 2019. The Hippo Pathway: Biology and Pathophysiology. In Annual review of biochemistry, 88, 577-604. doi:10.1146/annurev-biochem-013118-111829. https://pubmed.ncbi.nlm.nih.gov/30566373/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen