C57BL/6JCya-Igfbp3em1/Cya
Common Name:
Igfbp3-KO
Product ID:
S-KO-18417
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Igfbp3-KO
Strain ID
KOCMP-16009-Igfbp3-B6J-VB
Gene Name
Product ID
S-KO-18417
Gene Alias
IGFBP-3; IGgfbp3
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Igfbp3em1/Cya mice (Catalog S-KO-18417) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020702
NCBI RefSeq
NM_008343
Target Region
Exon 2~3
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Igfbp3, or Insulin-like growth factor binding protein 3, is a multifunctional protein. It binds to IGF-1, regulating growth, survival, and aging, and is involved in multiple pathways like the IGF-1 signaling pathway. It plays significant roles in various biological processes including cell growth, apoptosis, and tissue remodeling [2,3].
In cardiac fibrosis, IGFBP3 expression significantly increases in TGF-β1-stimulated human primary cardiac fibroblasts. Knockdown of IGFBP3 inhibits the migration and proliferation ability of these cells, suggesting its role in promoting cardiac fibroblast activation and fibrosis. METTL3 may regulate IGFBP3's expression and cardiac fibroblast activation via RNA epigenetic modifications [1]. In murine cells, IGFBP3 gene knockout (KO) by the Nuclease technology system enhanced the level of SA-β-gal staining and short telomere length, and increased the expression levels of senescence-related proteins, indicating its key role in the aging mechanism [3]. In human endometrial mesenchymal stem cells, IGFBP3-knockout cells lost stemness and underwent chondrogenic differentiation [2].
In conclusion, Igfbp3 is crucial for various biological functions such as cell growth, apoptosis, and tissue-specific differentiation. Studies using Igfbp3 KO models have provided insights into its roles in diseases like cardiac fibrosis and aging-related processes, highlighting its potential as a therapeutic target in these areas.
References:
1. Ding, Ji-Fei, Sun, He, Song, Kai, Xu, Sheng-Song, Tao, Hui. 2023. IGFBP3 epigenetic promotion induced by METTL3 boosts cardiac fibroblast activation and fibrosis. In European journal of pharmacology, 942, 175494. doi:10.1016/j.ejphar.2023.175494. https://pubmed.ncbi.nlm.nih.gov/36657656/
2. Ushakov, Roman E, Skvortsova, Elena V, Vitte, Mikhail A, Kenis, Vladimir M, Burova, Elena B. 2020. Chondrogenic differentiation followed IGFBP3 loss in human endometrial mesenchymal stem cells. In Biochemical and biophysical research communications, 531, 133-139. doi:10.1016/j.bbrc.2020.07.064. https://pubmed.ncbi.nlm.nih.gov/32782147/
3. Eom, Su Yeon, Kim, Moon-Moo. 2024. The effect of IGFBP3 gene knockout by the Nuclease technology system on the IGF-1 pathway in murine cells. In Archives of gerontology and geriatrics, 125, 105484. doi:10.1016/j.archger.2024.105484. https://pubmed.ncbi.nlm.nih.gov/38838451/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen