C57BL/6JCya-Ptgesem1/Cya
Common Name:
Ptges-KO
Product ID:
S-KO-18421
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ptges-KO
Strain ID
KOCMP-64292-Ptges-B6J-VB
Gene Name
Product ID
S-KO-18421
Gene Alias
2410099E23Rik; D2Ertd369e; Pges; mPGES; mPGES-1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ptgesem1/Cya mice (Catalog S-KO-18421) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000102852
NCBI RefSeq
NM_022415
Target Region
Exon 2
Size of Effective Region
~0.1 kb
Detailed Document
Overview of Gene Research
Ptges, known as prostaglandin E synthase, is a key enzyme in the arachidonic acid pathway responsible for the synthesis of prostaglandin E2 (PGE2). PGE2 is involved in multiple biological processes such as inflammation, cell growth, and immune response [1-6, 8-10]. Dysregulation of the Ptges-PGE2 axis has been associated with various diseases, highlighting its biological importance. Genetic models, like gene knockout mouse models, can be valuable for studying its functions.
In lung fibrosis, Ptges is upregulated and contributes to myofibroblast differentiation via the HIF-1α-dependent glycolysis pathway [1]. In colorectal cancer, depletion of Ptges enhances the sensitivity of cancer cells to 5-fluorouracil, as Ptges/PGE2 axis modulates glycolysis in these cells [2]. In Gprc5a-knockout mice, Ptges/PGE2 signaling is highly associated with lung tumorigenesis and metastasis mainly through immunosuppression [3]. In pancreatic ductal adenocarcinoma, higher Ptges expression is observed, correlated with metabolic and immune reprogramming [4]. In non-small cell lung cancer, dysregulated Ptges promoted tumor migration and metastasis, and knockdown of Ptges suppressed these features [5]. In osteosarcoma, FUBP1 confers lobaplatin resistance by transcriptionally activating Ptges and facilitating the arachidonic acid metabolic pathway [6].
In conclusion, Ptges, through the production of PGE2, is involved in critical biological processes related to fibrosis, cancer development, and drug resistance. The use of gene knockout models in mice has significantly contributed to understanding its role in these disease areas, providing insights into potential therapeutic targets for treating lung fibrosis, colorectal cancer, lung cancer, pancreatic cancer, and osteosarcoma.
References:
1. Lin, Min-Hsi, Lee, Yi-Chen, Liao, Jia-Bin, Chou, Chih-Yu, Yang, Yi-Fang. . PTGES is involved in myofibroblast differentiation via HIF-1α-dependent glycolysis pathway. In Journal of cellular and molecular medicine, 28, e70157. doi:10.1111/jcmm.70157. https://pubmed.ncbi.nlm.nih.gov/39417702/
2. Geng, Song, Zhan, Hao, Cao, Lianmeng, Geng, Longlong, Ren, Xiang. 2023. Targeting PTGES/PGE2 axis enhances sensitivity of colorectal cancer cells to 5-fluorouracil. In Biochemistry and cell biology = Biochimie et biologie cellulaire, 101, 501-512. doi:10.1139/bcb-2023-0101. https://pubmed.ncbi.nlm.nih.gov/37358009/
3. Wang, Tong, Jing, Bo, Xu, Dongliang, Zhou, Binhua P, Deng, Jiong. 2020. PTGES/PGE2 signaling links immunosuppression and lung metastasis in Gprc5a-knockout mouse model. In Oncogene, 39, 3179-3194. doi:10.1038/s41388-020-1207-6. https://pubmed.ncbi.nlm.nih.gov/32060421/
4. Murthy, Divya, Attri, Kuldeep S. 2023. PTGES Expression Is Associated with Metabolic and Immune Reprogramming in Pancreatic Ductal Adenocarcinoma. In International journal of molecular sciences, 24, . doi:10.3390/ijms24087304. https://pubmed.ncbi.nlm.nih.gov/37108468/
5. Wang, Tong, Jing, Bo, Sun, Beibei, Zhou, Binhua P, Deng, Jiong. 2019. Stabilization of PTGES by deubiquitinase USP9X promotes metastatic features of lung cancer via PGE2 signaling. In American journal of cancer research, 9, 1145-1160. doi:. https://pubmed.ncbi.nlm.nih.gov/31285948/
6. Ma, Qiong, Sun, Jin, Wang, Huan, Gong, Li, Zhang, Wei. 2023. Far upstream element-binding protein 1 confers lobaplatin resistance by transcriptionally activating PTGES and facilitating the arachidonic acid metabolic pathway in osteosarcoma. In MedComm, 4, e257. doi:10.1002/mco2.257. https://pubmed.ncbi.nlm.nih.gov/37180822/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen