C57BL/6JCya-Asb14em1/Cya
Common Name:
Asb14-KO
Product ID:
S-KO-18428
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Asb14-KO
Strain ID
KOCMP-142687-Asb14-B6J-VB
Gene Name
Product ID
S-KO-18428
Gene Alias
Asb-14
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Asb14em1/Cya mice (Catalog S-KO-18428) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000238987
NCBI RefSeq
NM_001170748.2
Target Region
Exon 3~5
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Asb14, short for Ankyrin Repeat And SOCS Box Containing 14, is an E3 ubiquitin ligase. The ubiquitin proteasome system it belongs to is crucial for regulating the cell cycle. It may be involved in pathways related to cardiac function and potentially epilepsy, as well as in processes related to metabolic syndrome [1,2,3]. Animal models, such as the GASH/Sal model of epilepsy, can be valuable for studying Asb14's role in disease-related genetic alterations [2].
In the context of cardiac regeneration, microarray analysis of apical resection ventricle tissues showed that ASB14 was down-regulated during the cardiac regenerative response. Silencing ASB14 in cardiomyocytes both in vitro and in vivo promoted nuclear proliferation. Mice with ASB14 deficiency had preserved cardiac function in response to ischemic injury due to enhanced cardiomyocyte nuclear proliferation. Mechanistically, ASB14 deficiency decreased the degradation of microtubule-associated protein RP/EB family member 2 (MAPRE2) protein, which promoted cardiomyocyte nuclear proliferation and enhanced cardiac repair after myocardial infarction [1].
In conclusion, Asb14, as an E3 ubiquitin ligase, plays a role in inhibiting cardiomyocyte proliferation through regulating MAPRE2 ubiquitination. The study of Asb14-deficient mouse models has provided insights into its potential as a target for treating heart failure induced by myocardial infarction injury [1].
References:
1. Yang, Yanpeng, Ma, Dongpu, Liu, Bo, Lv, Feifei, Qiu, Chunguang. 2024. E3 Ubiquitin Ligase ASB14 Inhibits Cardiomyocyte Proliferation by Regulating MAPRE2 Ubiquitination. In Cell biochemistry and biophysics, 82, 715-727. doi:10.1007/s12013-024-01223-x. https://pubmed.ncbi.nlm.nih.gov/38319584/
2. Díaz-Casado, Elena, Gómez-Nieto, Ricardo, de Pereda, José M, Jara-Acevedo, María, López, Dolores E. 2020. Analysis of gene variants in the GASH/Sal model of epilepsy. In PloS one, 15, e0229953. doi:10.1371/journal.pone.0229953. https://pubmed.ncbi.nlm.nih.gov/32168507/
3. Šedová, Lucie, Pravenec, Michal, Křenová, Drahomíra, Křen, Vladimír, Šeda, Ondřej. 2016. Isolation of a Genomic Region Affecting Most Components of Metabolic Syndrome in a Chromosome-16 Congenic Rat Model. In PloS one, 11, e0152708. doi:10.1371/journal.pone.0152708. https://pubmed.ncbi.nlm.nih.gov/27031336/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen