C57BL/6JCya-Ptdss1em1/Cya
Common Name:
Ptdss1-KO
Product ID:
S-KO-18441
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ptdss1-KO
Strain ID
KOCMP-19210-Ptdss1-B6J-VB
Gene Name
Product ID
S-KO-18441
Gene Alias
PSS-1; mKIAA0024
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
13
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ptdss1em1/Cya mice (Catalog S-KO-18441) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000021990
NCBI RefSeq
NM_008959
Target Region
Exon 4
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Ptdss1, encoding phosphatidylserine synthase 1, is an enzyme that catalyzes the conversion of phosphatidylcholine to phosphatidylserine, playing a key role in glycerophospholipid metabolism [1,3,5]. Glycerophospholipid metabolism is essential for cell membrane structure and function, and thus Ptdss1 is of great biological importance. Genetic models, such as transgenic zebrafish attempts to express wild-type and mutant Ptdss1, have been used to study its function, though transgene expression faced challenges [5].
In murine mammary tumors, depletion of Ptdss1 from tumor cells reduced ether-phosphatidylserine (ePS) levels, stunted tumor growth, and decreased tumor-associated macrophage (TAM) abundance [2]. In B cell lymphoma cell lines, inhibition of Ptdss1 caused an imbalance in the membrane phospholipidome, leading to B cell receptor hyperactivation and apoptotic cell death, and in a mouse xenograft model, it suppressed tumor growth [4]. These loss-of-function experiments in mouse models suggest Ptdss1's role in promoting tumor growth and maintaining a tumor-promoting microenvironment.
In conclusion, Ptdss1 is crucial for phosphatidylserine synthesis within the glycerophospholipid metabolism pathway. Model-based research, especially mouse models with Ptdss1 depletion, has revealed its significance in tumor-related processes, such as promoting tumor growth and modulating the tumor microenvironment, offering potential therapeutic targets for cancer treatment.
References:
1. Yang, Tao, Hui, Ruting, Nouws, Jessica, Zeng, Tianyang, Wu, Qingchen. 2022. Untargeted metabolomics analysis of esophageal squamous cell cancer progression. In Journal of translational medicine, 20, 127. doi:10.1186/s12967-022-03311-z. https://pubmed.ncbi.nlm.nih.gov/35287685/
2. Sekar, Divya, Dillmann, Christina, Sirait-Fischer, Evelyn, Brüne, Bernhard, Weigert, Andreas. . Phosphatidylserine Synthase PTDSS1 Shapes the Tumor Lipidome to Maintain Tumor-Promoting Inflammation. In Cancer research, 82, 1617-1632. doi:10.1158/0008-5472.CAN-20-3870. https://pubmed.ncbi.nlm.nih.gov/35425959/
3. Piard, Juliette, Lespinasse, James, Vlckova, Marketa, Sousa, Sérgio B, Van Maldergem, Lionel. 2018. Cutis laxa and excessive bone growth due to de novo mutations in PTDSS1. In American journal of medical genetics. Part A, 176, 668-675. doi:10.1002/ajmg.a.38604. https://pubmed.ncbi.nlm.nih.gov/29341480/
4. Omi, Jumpei, Kato, Taiga, Yoshihama, Yohei, Kono, Nozomu, Aoki, Junken. 2023. Phosphatidylserine synthesis controls oncogenic B cell receptor signaling in B cell lymphoma. In The Journal of cell biology, 223, . doi:10.1083/jcb.202212074. https://pubmed.ncbi.nlm.nih.gov/38048228/
5. Seda, Marian, Peskett, Emma, Demetriou, Charalambos, Stanier, Philip, Jenkins, Dagan. 2019. Analysis of transgenic zebrafish expressing the Lenz-Majewski syndrome gene PTDSS1 in skeletal cell lineages. In F1000Research, 8, 273. doi:10.12688/f1000research.17314.1. https://pubmed.ncbi.nlm.nih.gov/31231513/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen