C57BL/6JCya-Nup58em1/Cya
Common Name:
Nup58-KO
Product ID:
S-KO-18483
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Nup58-KO
Strain ID
KOCMP-71844-Nup58-B6J-VA
Gene Name
Product ID
S-KO-18483
Gene Alias
1700017F11Rik; Nupl1; mKIAA0410
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
14
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nup58em1/Cya mice (Catalog S-KO-18483) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000041905
NCBI RefSeq
NM_170591
Target Region
Exon 2
Size of Effective Region
~0.1 kb
Detailed Document
Overview of Gene Research
Nup58, a nucleoporin, is a component of the nuclear pore complex (NPC) [2,3,4,5,6,7,8,9,10]. NPCs act as gateways between the cytoplasm and nucleus in mammalian cells, facilitating the trafficking of proteins and RNA. Nup58 is part of the Nup62 subcomplex and is involved in multiple biological processes such as mitosis, stress response, and transposon silencing [1,4,8].
During mitosis, Nup58 localizes to the nuclear rim in interphase, and to mitotic spindles, centrosomes, and mid-bodies during mitosis. Depletion of Nup58 leads to centrosomal abnormalities and delayed abscission [1]. In lung adenocarcinoma, NUP58 is highly expressed in primary and metastatic foci. Knockdown of NUP58 inhibits metastasis and invasion of lung adenocarcinoma cell lines, and it is associated with the GSK-3β/Snail signaling pathway [2]. In maize, overexpression of ZmNUP58 promotes chlorophyll content and antioxidant enzyme activities under drought and salt stress, regulating genes related to phytohormone synthesis, osmotic adjustment, and more [4]. In Drosophila, loss of Nup54 and Nup58 in ovarian follicle cells compromises piRNA biogenesis from the flamenco locus, re-activating transposons [8].
In conclusion, Nup58 is crucial for normal cell division, stress response in plants, and transposon silencing in Drosophila. In cancer, it plays a role in promoting metastasis. These findings from different model-based research, including gene knockdown in cell lines and functional studies in plants and Drosophila, provide insights into its essential biological functions and its potential as a target in disease treatment, especially in lung adenocarcinoma [1,2,4,8].
References:
1. Hazawa, Masaharu, Lim, Kee Siang, Dewi, Firli R P, Kobayashi, Akiko, Wong, Richard W. 2019. Nucleoporin Nup58 localizes to centrosomes and mid-bodies during mitosis. In Cell division, 14, 7. doi:10.1186/s13008-019-0050-z. https://pubmed.ncbi.nlm.nih.gov/31388347/
2. Shi, Jianlin, Li, Chen, Wang, Hui, Xiao, Benshan, Qiu, Wanfang. 2019. NUP58 facilitates metastasis and epithelial-mesenchymal transition of lung adenocarcinoma via the GSK-3β/Snail signaling pathway. In American journal of translational research, 11, 393-405. doi:. https://pubmed.ncbi.nlm.nih.gov/30787996/
3. Danilov, Lavrentii G, Moskalenko, Svetlana E, Matveenko, Andrew G, Zhouravleva, Galina A, Bondarev, Stanislav A. 2021. The Human NUP58 Nucleoporin Can Form Amyloids In Vitro and In Vivo. In Biomedicines, 9, . doi:10.3390/biomedicines9101451. https://pubmed.ncbi.nlm.nih.gov/34680573/
4. Liu, Zhixue, Abou-Elwafa, Salah Fatouh, Xie, Jiarong, Ren, Zhenzhen, Wei, Li. 2022. A Nucleoporin NUP58 modulates responses to drought and salt stress in maize (Zea mays L.). In Plant science : an international journal of experimental plant biology, 320, 111296. doi:10.1016/j.plantsci.2022.111296. https://pubmed.ncbi.nlm.nih.gov/35643613/
5. Melcák, Ivo, Hoelz, André, Blobel, Günter. . Structure of Nup58/45 suggests flexible nuclear pore diameter by intermolecular sliding. In Science (New York, N.Y.), 315, 1729-32. doi:. https://pubmed.ncbi.nlm.nih.gov/17379812/
6. Sharma, Alok, Solmaz, Sozanne R, Blobel, Günter, Melčák, Ivo. 2015. Ordered Regions of Channel Nucleoporins Nup62, Nup54, and Nup58 Form Dynamic Complexes in Solution. In The Journal of biological chemistry, 290, 18370-8. doi:10.1074/jbc.M115.663500. https://pubmed.ncbi.nlm.nih.gov/26025361/
7. Danilov, Lavrentii G, Sukhanova, Xenia V, Rogoza, Tatiana M, Zhouravleva, Galina A, Bondarev, Stanislav A. 2023. Identification of New FG-Repeat Nucleoporins with Amyloid Properties. In International journal of molecular sciences, 24, . doi:10.3390/ijms24108571. https://pubmed.ncbi.nlm.nih.gov/37239918/
8. Munafò, Marzia, Lawless, Victoria R, Passera, Alessandro, Hannon, Gregory J, Czech, Benjamin. 2021. Channel nuclear pore complex subunits are required for transposon silencing in Drosophila. In eLife, 10, . doi:10.7554/eLife.66321. https://pubmed.ncbi.nlm.nih.gov/33856346/
9. Ferrández-Ayela, Almudena, Alonso-Peral, María Magdalena, Sánchez-García, Ana Belén, Micol, José Luis, Ponce, María Rosa. 2013. Arabidopsis TRANSCURVATA1 encodes NUP58, a component of the nucleopore central channel. In PloS one, 8, e67661. doi:10.1371/journal.pone.0067661. https://pubmed.ncbi.nlm.nih.gov/23840761/
10. Sonawane, Parshuram J, S Dewangan, Pravin, Madheshiya, Pankaj Kumar, Banerjee, Manidipa, Chauhan, Radha. 2020. Molecular and structural analysis of central transport channel in complex with Nup93 of nuclear pore complex. In Protein science : a publication of the Protein Society, 29, 2510-2527. doi:10.1002/pro.3983. https://pubmed.ncbi.nlm.nih.gov/33085133/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen