C57BL/6JCya-Mmgt1em1/Cya
Common Name:
Mmgt1-KO
Product ID:
S-KO-18508
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mmgt1-KO
Strain ID
KOCMP-236792-Mmgt1-B6J-VB
Gene Name
Product ID
S-KO-18508
Gene Alias
9630048L06Rik; Emc5; Mir5116; Tmem32; mir-5116
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mmgt1em1/Cya mice (Catalog S-KO-18508) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000059899
NCBI RefSeq
NM_146234
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Mmgt1, also known as membrane magnesium transporter 1, is a key component in magnesium (Mg²⁺) transport. It belongs to the "membrane Mg²⁺ transporters" (MMgTs) family, with proteins residing in the membrane. MMgT1 mediates Mg²⁺ transport, playing a crucial role in maintaining Mg²⁺ homeostasis, which is essential for numerous cellular functions. Its expression in various cells suggests a wide-spread significance in different biological processes [2].
In Mycobacterium tuberculosis (Mtb) infection, Mtb-infected MMGT1-deficient macrophages promoted a switch to persistence, upregulated lipid metabolism genes, and accumulated lipid droplets during infection. Targeting triacylglycerol synthesis reduced both droplet formation and Mtb persistence. The orphan G protein-coupled receptor GPR156 is a key inducer of droplet accumulation in ΔMMGT1 cells, uncovering the role of MMGT1-GPR156-lipid droplets in the induction of Mtb persistence [1].
In conclusion, Mmgt1 is vital for Mg²⁺ transport and homeostasis. Its deficiency in macrophages reveals its role in regulating Mtb persistence through the MMGT1-GPR156-lipid droplet axis, providing insights into the mechanisms underlying Mtb infection and potentially new strategies for treating tuberculosis.
References：
1. Kalam, Haroon, Chou, Chih-Hung, Kadoki, Motohiko, Hung, Deborah T, Xavier, Ramnik J. 2023. Identification of host regulators of Mycobacterium tuberculosis phenotypes uncovers a role for the MMGT1-GPR156 lipid droplet axis in persistence. In Cell host & microbe, 31, 978-992.e5. doi:10.1016/j.chom.2023.05.009. https://pubmed.ncbi.nlm.nih.gov/37269834/
2. Goytain, Angela, Quamme, Gary A. 2007. Identification and characterization of a novel family of membrane magnesium transporters, MMgT1 and MMgT2. In American journal of physiology. Cell physiology, 294, C495-502. doi:. https://pubmed.ncbi.nlm.nih.gov/18057121/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen