C57BL/6JCya-Mmgt1em1/Cya
Common Name
Mmgt1-KO
Product ID
S-KO-18508
Backgroud
C57BL/6JCya
Strain ID
KOCMP-236792-Mmgt1-B6J-VB
Status
When using this mouse strain in a publication, please cite “Mmgt1-KO Mouse (Catalog S-KO-18508) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Mmgt1-KO
Strain ID
KOCMP-236792-Mmgt1-B6J-VB
Gene Name
Product ID
S-KO-18508
Gene Alias
Emc5, Tmem32, Mir5116, mir-5116, 9630048L06Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr X
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000059899
NCBI RefSeq
NM_146234
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Overview of Gene Research
Mmgt1, also known as membrane magnesium transporter 1, is a key component in magnesium (Mg²⁺) transport. It belongs to the "membrane Mg²⁺ transporters" (MMgTs) family, with proteins residing in the membrane. MMgT1 mediates Mg²⁺ transport, playing a crucial role in maintaining Mg²⁺ homeostasis, which is essential for numerous cellular functions. Its expression in various cells suggests a wide-spread significance in different biological processes [2].
In Mycobacterium tuberculosis (Mtb) infection, Mtb-infected MMGT1-deficient macrophages promoted a switch to persistence, upregulated lipid metabolism genes, and accumulated lipid droplets during infection. Targeting triacylglycerol synthesis reduced both droplet formation and Mtb persistence. The orphan G protein-coupled receptor GPR156 is a key inducer of droplet accumulation in ΔMMGT1 cells, uncovering the role of MMGT1-GPR156-lipid droplets in the induction of Mtb persistence [1].
In conclusion, Mmgt1 is vital for Mg²⁺ transport and homeostasis. Its deficiency in macrophages reveals its role in regulating Mtb persistence through the MMGT1-GPR156-lipid droplet axis, providing insights into the mechanisms underlying Mtb infection and potentially new strategies for treating tuberculosis.
References：
1. Kalam, Haroon, Chou, Chih-Hung, Kadoki, Motohiko, Hung, Deborah T, Xavier, Ramnik J. 2023. Identification of host regulators of Mycobacterium tuberculosis phenotypes uncovers a role for the MMGT1-GPR156 lipid droplet axis in persistence. In Cell host & microbe, 31, 978-992.e5. doi:10.1016/j.chom.2023.05.009. https://pubmed.ncbi.nlm.nih.gov/37269834/
2. Goytain, Angela, Quamme, Gary A. 2007. Identification and characterization of a novel family of membrane magnesium transporters, MMgT1 and MMgT2. In American journal of physiology. Cell physiology, 294, C495-502. doi:. https://pubmed.ncbi.nlm.nih.gov/18057121/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.