C57BL/6JCya-Baz2bem1/Cya
Common Name:
Baz2b-KO
Product ID:
S-KO-18511
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Baz2b-KO
Strain ID
KOCMP-407823-Baz2b-B6J-VB
Gene Name
Product ID
S-KO-18511
Gene Alias
5830435C13Rik; D2Ertd794e
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Baz2bem1/Cya mice (Catalog S-KO-18511) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000112550
NCBI RefSeq
NM_001001182
Target Region
Exon 4~5
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
BAZ2B, also known as bromodomain adjacent to zinc finger 2B, is a regulatory subunit of the ISWI (Imitation Switch) remodeling complex and is involved in nucleosome remodeling [1]. It is a large multidomain protein containing epigenetic reader domains like the plant homeodomain (PHD), bromodomain (BRD), and a Tip5/ARBP/MBD (TAM) domain. BAZ2B has been implicated in various biological processes and diseases, playing a significant role in chromatin-related functions.
Loss-of-function and haploinsufficiency of BAZ2B are associated with multiple diseases. In humans, heterozygous loss-of-function variants in BAZ2B cause an autosomal dominant neurodevelopmental syndrome with incomplete penetrance. Phenotypes associated with BAZ2B loss-of function include developmental delay, intellectual disability, autism spectrum disorder, speech delay, behavioral abnormalities, seizures, vision-related issues, congenital heart defects, poor fetal growth, and dysmorphic features [2]. In mice, ablation of Baz2b, the mouse orthologue of human BAZ2B, attenuates age-dependent body-weight gain and prevents cognitive decline in ageing mice, suggesting its role in healthy ageing [3]. In the context of liver regeneration, in vivo CRISPR screening identified Baz2b as a regulator, with inhibition of Baz2b promoting liver regeneration. Distinct BAZ2-specific bromodomain inhibitors also led to accelerated liver healing after injuries and improved healing in an inflammatory bowel disease model [4]. In children with asthma, lnc-BAZ2B promotes M2 macrophage activation and inflammation by stabilizing BAZ2B pre-mRNA, and knockdown of Baz2b alleviated asthma severity in a mouse model [5]. Depletion of Baz2B in the Hap1 cell line resulted in altered cell morphology, reduced colony formation, and perturbed transcriptional profiles, though overall chromatin structure remained unchanged [6].
In conclusion, BAZ2B is crucial in processes such as neurodevelopment, healthy ageing, tissue regeneration, and inflammation. Research on BAZ2B, especially through gene knockout and other loss-of-function models in mice and in vitro cell studies, has provided insights into its role in various disease conditions like neurodevelopmental disorders, age-related cognitive decline, liver regeneration-related pathologies, and asthma. This understanding of BAZ2B's functions can potentially contribute to the development of therapeutic strategies for these diseases.
References:
1. Feng, Yingying, Chen, Sizhuo, Zhou, Mengqi, Min, Jinrong, Liu, Ke. 2022. Crystal structure of the BAZ2B TAM domain. In Heliyon, 8, e09873. doi:10.1016/j.heliyon.2022.e09873. https://pubmed.ncbi.nlm.nih.gov/35865993/
2. Sewani, Soha, Azamian, Mahshid S, Mendelsohn, Bryce A, Lalani, Seema R, Scott, Daryl A. 2023. Neurodevelopmental and other phenotypes recurrently associated with heterozygous BAZ2B loss-of-function variants. In American journal of medical genetics. Part A, 194, e63445. doi:10.1002/ajmg.a.63445. https://pubmed.ncbi.nlm.nih.gov/37872713/
3. Yuan, Jie, Chang, Si-Yuan, Yin, Shi-Gang, Jiang, Lubin, Cai, Shi-Qing. 2020. Two conserved epigenetic regulators prevent healthy ageing. In Nature, 579, 118-122. doi:10.1038/s41586-020-2037-y. https://pubmed.ncbi.nlm.nih.gov/32103178/
4. Jia, Yuemeng, Li, Lin, Lin, Yu-Hsuan, Ready, Joseph M, Zhu, Hao. 2022. In vivo CRISPR screening identifies BAZ2 chromatin remodelers as druggable regulators of mammalian liver regeneration. In Cell stem cell, 29, 372-385.e8. doi:10.1016/j.stem.2022.01.001. https://pubmed.ncbi.nlm.nih.gov/35090595/
5. Xia, Li, Wang, Xiang, Liu, Lijuan, Qian, Liling, Zhou, Yufeng. 2020. lnc-BAZ2B promotes M2 macrophage activation and inflammation in children with asthma through stabilizing BAZ2B pre-mRNA. In The Journal of allergy and clinical immunology, 147, 921-932.e9. doi:10.1016/j.jaci.2020.06.034. https://pubmed.ncbi.nlm.nih.gov/32712329/
6. Breindl, Matthias, Spitzer, Dominika, Gerasimaitė, Rūta, Lukinavičius, Gražvydas, Manelytė, Laura. . Biochemical and cellular insights into the Baz2B protein, a non-catalytic subunit of the chromatin remodeling complex. In Nucleic acids research, 52, 337-354. doi:10.1093/nar/gkad1096. https://pubmed.ncbi.nlm.nih.gov/38000389/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen