C57BL/6JCya-Hpcal1em1/Cya
Common Name:
Hpcal1-KO
Product ID:
S-KO-18522
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Hpcal1-KO
Strain ID
KOCMP-53602-Hpcal1-B6J-VA
Gene Name
Product ID
S-KO-18522
Gene Alias
NVL-3; NVP-3; Nvp3; VILIP3; Vnsl3; Vsnl3
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hpcal1em1/Cya mice (Catalog S-KO-18522) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000071858
NCBI RefSeq
NM_016677
Target Region
Exon 2~3
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Hpcal1, also known as hippocalcin like 1, serves as a calcium ion (Ca2+) sensor and is involved in multiple biological processes. It participates in selective autophagy, specifically acting as an autophagy receptor in ferroptosis, and is associated with pathways like Wnt/β-catenin signaling. It also has significance in cancer development, lipid metabolism, and may be a biomarker for certain diseases [1,2,3,4,5].
In a study using Hpcal1 null mice, Hpcal1 was identified as a negative regulator of de novo lipid biosynthesis and mTOR signaling activation, limiting liver tumorigenesis [4]. Genetic loss of Hpcal1 made hepatocellular carcinoma (HCC) mTORC1-addicted and sensitive to mTOR inhibitor AZD-8055 in vitro and in vivo. Additionally, the genetic or pharmacological inhibition of Hpcal1 prevented ferroptosis-induced tumor suppression and pancreatitis in mouse models [1].
In conclusion, Hpcal1 plays essential roles in processes such as autophagy-dependent ferroptosis, lipid metabolism, and cancer-related signaling pathways. Mouse models with Hpcal1 knockout or knockdown have revealed its significance in liver tumorigenesis and ferroptosis-related diseases, providing insights into potential therapeutic strategies for these conditions.
References：
1. Chen, Xin, Song, Xinxin, Li, Jingbo, Tang, Daolin, Kang, Rui. 2022. Identification of HPCAL1 as a specific autophagy receptor involved in ferroptosis. In Autophagy, 19, 54-74. doi:10.1080/15548627.2022.2059170. https://pubmed.ncbi.nlm.nih.gov/35403545/
2. Zhang, Dongming, Liu, Xidong, Xu, Xuebin, Shan, Baochang, Liu, Bing. 2019. HPCAL1 promotes glioblastoma proliferation via activation of Wnt/β-catenin signalling pathway. In Journal of cellular and molecular medicine, 23, 3108-3117. doi:10.1111/jcmm.14083. https://pubmed.ncbi.nlm.nih.gov/30843345/
3. Ma, Mingjian, Zeng, Guangyan, Li, Jinhui, Chen, Jiancong, Lai, Jiaming. 2022. Expressional and prognostic value of HPCAL1 in cholangiocarcinoma via integrated bioinformatics analyses and experiments. In Cancer medicine, 12, 824-836. doi:10.1002/cam4.4897. https://pubmed.ncbi.nlm.nih.gov/35645147/
4. Chen, Tao, Yuan, Zhiqing, Lei, Zhou, Li, Qiwei, Zhang, Yonglong. 2022. Hippocalcin-Like 1 blunts liver lipid metabolism to suppress tumorigenesis via directly targeting RUVBL1-mTOR signaling. In Theranostics, 12, 7450-7464. doi:10.7150/thno.75936. https://pubmed.ncbi.nlm.nih.gov/36438486/
5. Wu, Zenan, Chen, Huan, Ke, Shiwen, Zhu, Wei, Liu, Liangji. 2023. Identifying potential biomarkers of idiopathic pulmonary fibrosis through machine learning analysis. In Scientific reports, 13, 16559. doi:10.1038/s41598-023-43834-z. https://pubmed.ncbi.nlm.nih.gov/37783761/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen