C57BL/6JCya-Ccdc188em1/Cya
Common Name:
Ccdc188-KO
Product ID:
S-KO-18527
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ccdc188-KO
Strain ID
KOCMP-102638083-Ccdc188-B6J-VA
Gene Name
Product ID
S-KO-18527
Gene Alias
EG665975; Gm7873
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
16
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ccdc188em1/Cya mice (Catalog S-KO-18527) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000231369
NCBI RefSeq
XM_011246064
Target Region
Exon 1~8
Size of Effective Region
~3.3 kb
Detailed Document
Overview of Gene Research
Ccdc188, also known as coiled-coil domain containing 188, is a gene with testis-enriched expression. It is crucial for male fertility and is likely involved in pathways related to spermatozoa development [1]. The study of its function has been facilitated by genetic models, especially knockout mouse models.
Using the Nuclease technology system to generate a Ccdc188 knockout mouse line, it was found that Ccdc188 knockout male mice were sterile. Most of the Ccdc188-null spermatozoa were acephalic. Transmission electron microscopy showed that although the sperm head-to-tail coupling apparatus could assemble without CCDC188, it failed to attach to the nucleus during spermiogenesis, leading to sperm head and neck separation. Also, almost all spermatozoa in the cauda epididymis lacked a mitochondrial sheath [1].
In conclusion, CCDC188 plays a vital role in forming a tight sperm head-neck junction, which is essential for male fertility. The Ccdc188 knockout mouse model has significantly contributed to understanding the molecular mechanism underlying male infertility caused by acephalic spermatozoa syndrome [1].
References:
1. Qiu, Yumiao, Shimada, Keisuke, Yamamoto, Kaito, Ikawa, Masahito. . Loss of CCDC188 causes male infertility with defects in the sperm head-neck connection in mice†. In Biology of reproduction, 112, 169-178. doi:10.1093/biolre/ioae137. https://pubmed.ncbi.nlm.nih.gov/39292630/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen