C57BL/6JCya-Mzb1em1/Cya
Common Name:
Mzb1-KO
Product ID:
S-KO-18531
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mzb1-KO
Strain ID
KOCMP-69816-Mzb1-B6J-VA
Gene Name
Product ID
S-KO-18531
Gene Alias
2010001M09Rik; PACAP; pERp1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mzb1em1/Cya mice (Catalog S-KO-18531) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025211
NCBI RefSeq
NM_027222
Target Region
Exon 2~4
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
MZB1, also known as Marginal zone B and B-1 cell-specific protein 1, is an endoplasmic reticulum-resident protein preferentially expressed in plasma cells, marginal zone and B1 B cells [3]. It plays a crucial role in the humoral immune response, promoting the secretion of IgM and IgA by interacting with the tail piece of their heavy chains [3]. It also affects cellular processes like proliferation, apoptosis, and mitochondrial function, and is associated with pathways such as PI3K-Akt [1].
In gene-knockout related studies, Mzb1-/-mice showed increased CRC development and progression, associated with reduced intestinal IgA levels, altered gut flora, and more severe gut and systemic inflammation, indicating MZB1-mediated IgA secretion suppresses CRC triggered by gut inflammation [2]. In atherosclerotic plaque vulnerability study, downregulation of Mzb1 in mouse models increased ox-LDL-induced apoptosis and cholesterol levels in human vascular smooth muscle cells, while Mzb1 overexpression alleviated these effects and attenuated atherosclerotic plaque vulnerability [4]. In myocardial infarction mouse models, Mzb1 expression was reduced in the infarct myocardium. Knockdown of Mzb1 in neonatal mouse ventricular cardiomyocytes decreased mitochondrial membrane potential and promoted apoptosis, while overexpression improved mitochondrial function and inhibited apoptosis [5].
In conclusion, MZB1 is essential for immunoglobulin secretion, cellular proliferation, apoptosis regulation, and mitochondrial function. Gene-knockout mouse models have revealed its significant role in diseases such as colorectal cancer, atherosclerosis, and myocardial infarction, helping to understand the biological functions and disease mechanisms related to MZB1, which may provide potential therapeutic targets for these diseases.
References:
1. Xu, Mengtao, Feng, Yong, Xiang, Xuelian, Liu, Li, Tang, Guodu. 2024. MZB1 regulates cellular proliferation, mitochondrial dysfunction, and inflammation and targets the PI3K-Akt signaling pathway in acute pancreatitis. In Cellular signalling, 118, 111143. doi:10.1016/j.cellsig.2024.111143. https://pubmed.ncbi.nlm.nih.gov/38508349/
2. Tang, Yue, Feng, Xiaoqian, Lu, Qing, Hase, Koji, Wang, Ji-Yang. 2023. MZB1-mediated IgA secretion suppresses the development and progression of colorectal cancer triggered by gut inflammation. In Mucosal immunology, 17, 450-460. doi:10.1016/j.mucimm.2023.12.002. https://pubmed.ncbi.nlm.nih.gov/38101774/
3. Geary, Benjamin, Sun, Bo, Tilvawala, Ronak R, Thompson, Paul R, Ho, I-Cheng. 2023. Peptidylarginine deiminase 2 citrullinates MZB1 and promotes the secretion of IgM and IgA. In Frontiers in immunology, 14, 1290585. doi:10.3389/fimmu.2023.1290585. https://pubmed.ncbi.nlm.nih.gov/38094295/
4. Zhu, Guanglang, Li, Yang, Gao, Hongxia, Fan, Heyu, Fan, Longhua. 2024. Mzb1 Attenuates Atherosclerotic Plaque Vulnerability in ApoE-/- Mice by Alleviating Apoptosis and Modulating Mitochondrial Function. In Journal of cardiovascular translational research, 17, 782-794. doi:10.1007/s12265-024-10483-0. https://pubmed.ncbi.nlm.nih.gov/38294627/
5. Zhang, Lu, Wang, Yi-Ning, Ju, Jia-Ming, Liang, Hai-Hai, Yang, Bao-Feng. 2020. Mzb1 protects against myocardial infarction injury in mice via modulating mitochondrial function and alleviating inflammation. In Acta pharmacologica Sinica, 42, 691-700. doi:10.1038/s41401-020-0489-0. https://pubmed.ncbi.nlm.nih.gov/32759964/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen