C57BL/6JCya-Slc7a5em1/Cya
Common Name:
Slc7a5-KO
Product ID:
S-KO-18533
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Slc7a5-KO
Strain ID
KOCMP-20539-Slc7a5-B6J-VA
Gene Name
Product ID
S-KO-18533
Gene Alias
4F2LC; D0H16S474E; Gm42049; LAT1; TA1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc7a5em1/Cya mice (Catalog S-KO-18533) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000045557
NCBI RefSeq
NM_011404
Target Region
Exon 3
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Slc7a5, also known as LAT1, is an amino acid transporter that forms a heterodimeric complex with SLC3A2 (4F2hc) [4]. It is crucial for the efficient uptake of essential amino acids and hormones, promoting cellular growth by stimulating mTORC1 signalling and repressing the integrated stress response (ISR) [4].
Cancer cells highly express Slc7a5, and its high expression is linked to poor patient prognosis. Pharmacologic inhibition and knockdown/knockout of Slc7a5 suppress cancer cell proliferation and xenograft tumor growth [1]. In triple-negative breast cancer, knocking down Slc7a5 inhibits cell proliferation, migration, and invasion, and increases CD8+ T-cell infiltration [5]. In gastric cancer, circARID1A promotes cancer proliferation by forming a circARID1A-IGF2BP3-SLC7A5 RNA-protein ternary complex [2]. In lung cancer, IGF2BP2 enhances SLC7A5 mRNA stability and translation, and this positive feedback loop promotes radioresistance, while FBW7/GSK3β can degrade IGF2BP2 to inhibit this loop [3]. In systemic lupus erythematosus, SLC7A5 expression is up-regulated in peripheral blood T and B lymphocytes and is associated with renal damage [6]. In head and neck squamous cell carcinoma, TP63 transcriptionally regulates SLC7A5 to suppress ferroptosis [7]. In triple-negative breast cancer, the SLC7A5/E2F1/PTBP1/PKM2 axis mediates cancer progression and therapy effect through the crosstalk of amino acid metabolism and glycolysis pathway [8].
In conclusion, Slc7a5 is essential for amino acid uptake and cellular growth regulation. Model-based research, especially knockout/knockdown experiments, has revealed its significant roles in various diseases, particularly in cancer progression, immune-related diseases like systemic lupus erythematosus, and ferroptosis regulation in head and neck squamous cell carcinoma. Understanding Slc7a5 provides potential therapeutic targets for these diseases.
References:
1. Kanai, Yoshikatsu. 2021. Amino acid transporter LAT1 (SLC7A5) as a molecular target for cancer diagnosis and therapeutics. In Pharmacology & therapeutics, 230, 107964. doi:10.1016/j.pharmthera.2021.107964. https://pubmed.ncbi.nlm.nih.gov/34390745/
2. Ma, Qiang, Yang, Feifei, Huang, Bo, Wang, Shimin, Xiao, Bin. 2022. CircARID1A binds to IGF2BP3 in gastric cancer and promotes cancer proliferation by forming a circARID1A-IGF2BP3-SLC7A5 RNA-protein ternary complex. In Journal of experimental & clinical cancer research : CR, 41, 251. doi:10.1186/s13046-022-02466-3. https://pubmed.ncbi.nlm.nih.gov/35986300/
3. Zhou, Zhiyuan, Zhang, Bin, Deng, Yue, Wan, Chao, Yang, Kunyu. 2024. FBW7/GSK3β mediated degradation of IGF2BP2 inhibits IGF2BP2-SLC7A5 positive feedback loop and radioresistance in lung cancer. In Journal of experimental & clinical cancer research : CR, 43, 34. doi:10.1186/s13046-024-02959-3. https://pubmed.ncbi.nlm.nih.gov/38281999/
4. Kahlhofer, Jennifer, Teis, David. 2022. The human LAT1-4F2hc (SLC7A5-SLC3A2) transporter complex: Physiological and pathophysiological implications. In Basic & clinical pharmacology & toxicology, 133, 459-472. doi:10.1111/bcpt.13821. https://pubmed.ncbi.nlm.nih.gov/36460306/
5. Huang, Renhong, Wang, Han, Hong, Jin, Shen, Kunwei, Wang, Zheng. 2023. Targeting glutamine metabolic reprogramming of SLC7A5 enhances the efficacy of anti-PD-1 in triple-negative breast cancer. In Frontiers in immunology, 14, 1251643. doi:10.3389/fimmu.2023.1251643. https://pubmed.ncbi.nlm.nih.gov/37731509/
6. Tian, Juan, Li, Xiaowei, Jiang, Yiru, Meng, Liesu, Lu, Shemin. 2022. SLC7A5 expression is up-regulated in peripheral blood T and B lymphocytes of systemic lupus erythematosus patients, associating with renal damage. In Clinical immunology (Orlando, Fla.), 237, 108987. doi:10.1016/j.clim.2022.108987. https://pubmed.ncbi.nlm.nih.gov/35346864/
7. Chen, Zilong, Cai, Haoxi, Ye, Weiwei, Cai, Chengfu, Cai, Gengming. 2024. TP63 transcriptionally regulates SLC7A5 to suppress ferroptosis in head and neck squamous cell carcinoma. In Frontiers in immunology, 15, 1445472. doi:10.3389/fimmu.2024.1445472. https://pubmed.ncbi.nlm.nih.gov/39234254/
8. Jiang, Chengfei, Qian, Yingchen, Bai, Xiaoming, Liu, Bingjie, Jiang, Bing-Hua. 2025. SLC7A5/E2F1/PTBP1/PKM2 axis mediates progression and therapy effect of triple-negative breast cancer through the crosstalk of amino acid metabolism and glycolysis pathway. In Cancer letters, 617, 217612. doi:10.1016/j.canlet.2025.217612. https://pubmed.ncbi.nlm.nih.gov/40054655/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen