C57BL/6JCya-Mstnem1/Cya
Common Name:
Mstn-KO
Product ID:
S-KO-18536
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Mstn-KO
Strain ID
KOCMP-17700-Mstn-B6J-VA
Gene Name
Product ID
S-KO-18536
Gene Alias
Cmpt; Gdf8
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mstnem1/Cya mice (Catalog S-KO-18536) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027269
NCBI RefSeq
NM_010834
Target Region
Exon 1
Size of Effective Region
~0.5 kb
Detailed Document
Overview of Gene Research
Myostatin (MSTN), also known as Growth Differentiation Factor 8 (GDF-8), is a member of the transforming growth factor-β superfamily. It is a negative regulator of skeletal muscle growth and development, inhibiting the proliferation of myoblasts. Mutations in MSTN can lead to extensive skeletal muscle hyperplasia and hypertrophy, resulting in a "double-muscle" phenotype [1]. It is also involved in regulating energy homeostasis [5].
Mutations of the MSTN gene in various animals have been studied. In goats, TALENs technology was used to modify the MSTN gene, obtaining mutant cell lines and cloned goats with "double-muscle" phenotypes, which had increased muscle content [1]. In sheep, a MSTNDel73C mutation with FGF5 knockout via Nuclease technology led to a dominant "double-muscle" phenotype, promoting skeletal muscle myofiber hyperplasia [2]. In Hu sheep, the C-CRISPR system was used to generate MSTN-edited sheep with a double-muscled phenotype and enhanced AKT and suppressed ERK1/2 signaling in gluteus muscle [6]. In cattle, MSTN knockout cattle were produced via CRISPR-Cas9, and the MSTN mutation was transmitted from germ cells [4]. In athletes, the MSTN rs1805086 polymorphism was associated with the strength phenotype [3].
In conclusion, MSTN is a crucial negative regulator of skeletal muscle growth. Studies using gene-edited animal models have revealed its significant role in muscle development, energy homeostasis, and its potential association with athletic performance. These findings may have implications for livestock breeding, muscle-related disease treatment, and understanding human physical capabilities [1,2,3,4,5,6].
References:
1. Song, Shao-Zheng, He, Zheng-Yi, Cheng, Yong, Zhang, Ting, Li, Dan. . MSTN modification in goat mediated by TALENs and performance analysis. In Yi chuan = Hereditas, 44, 531-542. doi:10.16288/j.yczz.22-011. https://pubmed.ncbi.nlm.nih.gov/35729101/
2. Chen, Ming-Ming, Zhao, Yue, Yu, Kun, Lian, Di, Lian, Zheng-Xing. 2024. A MSTNDel73C mutation with FGF5 knockout sheep by Nuclease technology promotes skeletal muscle myofiber hyperplasia. In eLife, 12, . doi:10.7554/eLife.86827. https://pubmed.ncbi.nlm.nih.gov/39365728/
3. Kruszewski, Marek, Aksenov, Maksim Olegovich. 2022. Association of Myostatin Gene Polymorphisms with Strength and Muscle Mass in Athletes: A Systematic Review and Meta-Analysis of the MSTN rs1805086 Mutation. In Genes, 13, . doi:10.3390/genes13112055. https://pubmed.ncbi.nlm.nih.gov/36360291/
4. Gim, Gyeong-Min, Uhm, Kyeong-Hyun, Kwon, Dong-Hyeok, Lee, Won-Wu, Jang, Goo. 2022. Germline transmission of MSTN knockout cattle via CRISPR-Cas9. In Theriogenology, 192, 22-27. doi:10.1016/j.theriogenology.2022.08.021. https://pubmed.ncbi.nlm.nih.gov/36037573/
5. Wang, Hui, Guo, Shanshan, Gao, Huanqing, Liu, Tiemin, Kong, Xingxing. 2024. Myostatin regulates energy homeostasis through autocrine- and paracrine-mediated microenvironment communication. In The Journal of clinical investigation, 134, . doi:10.1172/JCI178303. https://pubmed.ncbi.nlm.nih.gov/38889010/
6. Guo, Rihong, Wang, Huili, Meng, Chunhua, Zhong, Jifeng, Cao, Shaoxian. 2023. Efficient and Specific Generation of MSTN-Edited Hu Sheep Using C-CRISPR. In Genes, 14, . doi:10.3390/genes14061216. https://pubmed.ncbi.nlm.nih.gov/37372396/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen