Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Mstnem1/Cya
Common Name:
Mstn-KO
Product ID:
S-KO-18537
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Mstn-KO
Strain ID
KOCMP-17700-Mstn-B6J-VB
Gene Name
Mstn
Product ID
S-KO-18537
Gene Alias
Cmpt; Gdf8
Background
C57BL/6JCya
NCBI ID
17700
Modification
Conventional knockout
Chromosome
1
Phenotype
MGI:95691
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mstnem1/Cya mice (Catalog S-KO-18537) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027269
NCBI RefSeq
NM_010834
Target Region
Exon 3
Size of Effective Region
~1.8 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Myostatin (MSTN), also known as Growth Differentiation Factor-8 (GDF-8), is a member of the transforming growth factor-β superfamily. It is a negative regulator of skeletal muscle growth and development, inhibiting the proliferation of myoblasts. MSTN is crucial for maintaining the proper balance of muscle mass and is also involved in metabolism [2,4,5]. Genetic models, such as gene-edited animals, are valuable for studying its functions.

In livestock, MSTN-edited pigs (MSTN+/-) have higher muscle depth and lower fat depth without developmental problems, and there are no significant differences in the histomorphology of digestive and reproductive organs compared to wild-type pigs [1]. In goats, mutations of the MSTN gene induced by TALENs technology cause extensive skeletal muscle hyperplasia and hypertrophy, resulting in "double muscle" symptoms, reducing fat differentiation and increasing muscle content [2]. In sheep, a MSTNDel73C mutation with FGF5 knockout produced by Nuclease technology shows a dominant "double-muscle" phenotype, promoting skeletal muscle myofiber hyperplasia through the MEK-ERK-FOSL1 axis [3]. In Hu sheep, MSTN-edited using the C-CRISPR system have a double-muscled phenotype, with enhanced AKT and suppressed ERK1/2 signaling in the gluteus muscle [6].

In conclusion, MSTN is a key negative regulator of skeletal muscle growth and also plays a role in metabolism. Gene-edited animal models, especially those with MSTN mutations, have demonstrated its importance in enhancing muscle mass and potentially improving meat production in livestock. This research provides a foundation for understanding the biological functions of MSTN and its potential applications in animal breeding and muscle-related disease treatment.

References:
1. Pei, Yangli, Fan, Ziyao, Song, Yuxin, Li, Hua, Li, Kui. 2022. Viscera Characteristics of MSTN-Edited Heterozygous Pigs. In Frontiers in genetics, 13, 764965. doi:10.3389/fgene.2022.764965. https://pubmed.ncbi.nlm.nih.gov/35299949/
2. Song, Shao-Zheng, He, Zheng-Yi, Cheng, Yong, Zhang, Ting, Li, Dan. . MSTN modification in goat mediated by TALENs and performance analysis. In Yi chuan = Hereditas, 44, 531-542. doi:10.16288/j.yczz.22-011. https://pubmed.ncbi.nlm.nih.gov/35729101/
3. Chen, Ming-Ming, Zhao, Yue, Yu, Kun, Lian, Di, Lian, Zheng-Xing. 2024. A MSTNDel73C mutation with FGF5 knockout sheep by Nuclease technology promotes skeletal muscle myofiber hyperplasia. In eLife, 12, . doi:10.7554/eLife.86827. https://pubmed.ncbi.nlm.nih.gov/39365728/
4. Lee, Se-Jin. . Targeting the myostatin signaling pathway to treat muscle loss and metabolic dysfunction. In The Journal of clinical investigation, 131, . doi:10.1172/JCI148372. https://pubmed.ncbi.nlm.nih.gov/33938454/
5. Wang, Hui, Guo, Shanshan, Gao, Huanqing, Liu, Tiemin, Kong, Xingxing. 2024. Myostatin regulates energy homeostasis through autocrine- and paracrine-mediated microenvironment communication. In The Journal of clinical investigation, 134, . doi:10.1172/JCI178303. https://pubmed.ncbi.nlm.nih.gov/38889010/
6. Guo, Rihong, Wang, Huili, Meng, Chunhua, Zhong, Jifeng, Cao, Shaoxian. 2023. Efficient and Specific Generation of MSTN-Edited Hu Sheep Using C-CRISPR. In Genes, 14, . doi:10.3390/genes14061216. https://pubmed.ncbi.nlm.nih.gov/37372396/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest