C57BL/6JCya-Mstnem1/Cya
Common Name:
Mstn-KO
Product ID:
S-KO-18537
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Mstn-KO
Strain ID
KOCMP-17700-Mstn-B6J-VB
Gene Name
Product ID
S-KO-18537
Gene Alias
Cmpt; Gdf8
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mstnem1/Cya mice (Catalog S-KO-18537) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027269
NCBI RefSeq
NM_010834
Target Region
Exon 3
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Myostatin (MSTN), also known as Growth Differentiation Factor-8 (GDF-8), is a member of the transforming growth factor-β superfamily. It is a negative regulator of skeletal muscle growth and development, inhibiting the proliferation of myoblasts. MSTN is crucial for maintaining the proper balance of muscle mass and is also involved in metabolism [2,4,5]. Genetic models, such as gene-edited animals, are valuable for studying its functions.
In livestock, MSTN-edited pigs (MSTN+/-) have higher muscle depth and lower fat depth without developmental problems, and there are no significant differences in the histomorphology of digestive and reproductive organs compared to wild-type pigs [1]. In goats, mutations of the MSTN gene induced by TALENs technology cause extensive skeletal muscle hyperplasia and hypertrophy, resulting in "double muscle" symptoms, reducing fat differentiation and increasing muscle content [2]. In sheep, a MSTNDel73C mutation with FGF5 knockout produced by Nuclease technology shows a dominant "double-muscle" phenotype, promoting skeletal muscle myofiber hyperplasia through the MEK-ERK-FOSL1 axis [3]. In Hu sheep, MSTN-edited using the C-CRISPR system have a double-muscled phenotype, with enhanced AKT and suppressed ERK1/2 signaling in the gluteus muscle [6].
In conclusion, MSTN is a key negative regulator of skeletal muscle growth and also plays a role in metabolism. Gene-edited animal models, especially those with MSTN mutations, have demonstrated its importance in enhancing muscle mass and potentially improving meat production in livestock. This research provides a foundation for understanding the biological functions of MSTN and its potential applications in animal breeding and muscle-related disease treatment.
References:
1. Pei, Yangli, Fan, Ziyao, Song, Yuxin, Li, Hua, Li, Kui. 2022. Viscera Characteristics of MSTN-Edited Heterozygous Pigs. In Frontiers in genetics, 13, 764965. doi:10.3389/fgene.2022.764965. https://pubmed.ncbi.nlm.nih.gov/35299949/
2. Song, Shao-Zheng, He, Zheng-Yi, Cheng, Yong, Zhang, Ting, Li, Dan. . MSTN modification in goat mediated by TALENs and performance analysis. In Yi chuan = Hereditas, 44, 531-542. doi:10.16288/j.yczz.22-011. https://pubmed.ncbi.nlm.nih.gov/35729101/
3. Chen, Ming-Ming, Zhao, Yue, Yu, Kun, Lian, Di, Lian, Zheng-Xing. 2024. A MSTNDel73C mutation with FGF5 knockout sheep by Nuclease technology promotes skeletal muscle myofiber hyperplasia. In eLife, 12, . doi:10.7554/eLife.86827. https://pubmed.ncbi.nlm.nih.gov/39365728/
4. Lee, Se-Jin. . Targeting the myostatin signaling pathway to treat muscle loss and metabolic dysfunction. In The Journal of clinical investigation, 131, . doi:10.1172/JCI148372. https://pubmed.ncbi.nlm.nih.gov/33938454/
5. Wang, Hui, Guo, Shanshan, Gao, Huanqing, Liu, Tiemin, Kong, Xingxing. 2024. Myostatin regulates energy homeostasis through autocrine- and paracrine-mediated microenvironment communication. In The Journal of clinical investigation, 134, . doi:10.1172/JCI178303. https://pubmed.ncbi.nlm.nih.gov/38889010/
6. Guo, Rihong, Wang, Huili, Meng, Chunhua, Zhong, Jifeng, Cao, Shaoxian. 2023. Efficient and Specific Generation of MSTN-Edited Hu Sheep Using C-CRISPR. In Genes, 14, . doi:10.3390/genes14061216. https://pubmed.ncbi.nlm.nih.gov/37372396/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen