C57BL/6JCya-Hnrnprem1/Cya
Common Name:
Hnrnpr-KO
Product ID:
S-KO-18547
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Hnrnpr-KO
Strain ID
KOCMP-74326-Hnrnpr-B6J-VB
Gene Name
Product ID
S-KO-18547
Gene Alias
2610003J05Rik; 2610528B01Rik; Hnrpr
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hnrnprem1/Cya mice (Catalog S-KO-18547) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000148843
NCBI RefSeq
NM_028871
Target Region
Exon 6~11
Size of Effective Region
~14.2 kb
Detailed Document
Overview of Gene Research
Hnrnpr, a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family, is an RNA-binding protein primarily functioning in the spliceosome C complex. It is involved in multiple biological processes such as alternative splicing, mRNA stability regulation, and gene expression control, which are crucial for normal development and cellular functions [3,5].
Knockdown of Hnrnpr in two types of mouse models with gastric cancer tumors decreased tumor aggressiveness and metastasis. Mechanistically, Hnrnpr promotes gastric cancer cell proliferation, migration, and invasion by stabilizing the mRNA levels of CCNB1 and CENPF [1]. In hepatocellular carcinoma, Hnrnpr binds to UPF3B pre-mRNA to generate an oncogenic splice variant UPF3B-S, which promotes cancer cell invasion and metastasis [2]. In neuroblastoma, Hnrnpr binds to and stabilizes ASCL1 mRNA in an m6A-dependent manner, promoting neuroblastoma progression [4].
In conclusion, Hnrnpr plays essential roles in various biological processes and disease conditions. Mouse models with Hnrnpr knockdown have revealed its significant impact on cancer-related processes, including proliferation, migration, and invasion in gastric, liver, and neuroblastoma cancers, highlighting its potential as a therapeutic target in these disease areas.
References：
1. Chen, Er-Bao, Qin, Xuan, Peng, Ke, Gan, Lu, Liu, Tian-Shu. 2019. HnRNPR-CCNB1/CENPF axis contributes to gastric cancer proliferation and metastasis. In Aging, 11, 7473-7491. doi:10.18632/aging.102254. https://pubmed.ncbi.nlm.nih.gov/31527303/
2. Wang, Hong, Qian, Dong, Wang, Jiabei, Zhang, Chris Zhiyi, Liu, Lianxin. 2024. HnRNPR-mediated UPF3B mRNA splicing drives hepatocellular carcinoma metastasis. In Journal of advanced research, 68, 257-270. doi:10.1016/j.jare.2024.02.010. https://pubmed.ncbi.nlm.nih.gov/38402949/
3. Duijkers, Floor A, McDonald, Andrew, Janssens, Georges E, Houtkooper, Riekelt H, MacInnes, Alyson W. 2019. HNRNPR Variants that Impair Homeobox Gene Expression Drive Developmental Disorders in Humans. In American journal of human genetics, 104, 1040-1059. doi:10.1016/j.ajhg.2019.03.024. https://pubmed.ncbi.nlm.nih.gov/31079900/
4. Hu, Ting, Zeng, Chong, Song, Zhihao, Ma, Qianquan, Li, Haoyu. 2024. HNRNPA2B1 and HNRNPR stabilize ASCL1 in an m6A-dependent manner to promote neuroblastoma progression. In Biochimica et biophysica acta. Molecular basis of disease, 1870, 167050. doi:10.1016/j.bbadis.2024.167050. https://pubmed.ncbi.nlm.nih.gov/38331110/
5. Jiang, Tao, Qu, Ruobing, Liu, Xuan, Wang, Li, Hua, Yimin. 2023. HnRNPR strongly represses splicing of a critical exon associated with spinal muscular atrophy through binding to an exonic AU-rich element. In Journal of medical genetics, 60, 1105-1115. doi:10.1136/jmg-2023-109186. https://pubmed.ncbi.nlm.nih.gov/37225410/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen