C57BL/6JCya-Elf3em1/Cya
Common Name:
Elf3-KO
Product ID:
S-KO-18559
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Elf3-KO
Strain ID
KOCMP-13710-Elf3-B6J-VA
Gene Name
Product ID
S-KO-18559
Gene Alias
ESE-1; ESX; jen
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Elf3em1/Cya mice (Catalog S-KO-18559) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003135
NCBI RefSeq
NM_001163131
Target Region
Exon 2~8
Size of Effective Region
~3.5 kb
Detailed Document
Overview of Gene Research
ELF3, also known as E74-like factor 3, is an important member of the E-twenty-six (ETS) transcription factor family. It plays diverse roles in various organisms. In plants, it is crucial for photoperiodic flowering, regulation of circadian rhythms, and acts as a thermosensor. In animals, it is involved in cell proliferation, differentiation, apoptosis, migration, and invasion, regulating a variety of biological behaviors [2,3].
In Arabidopsis, ELF3 contains a polyglutamine repeat within a prion-like domain, which functions as a thermosensor. The length of the polyQ repeat correlates with thermal responsiveness. ELF3 also forms the evening complex with ELF4 and LUX, regulating the transcription of circadian-clock related genes [1,7]. In zebrafish, Elf3 is required for epidermal, mesenchymal, and neural tissue development, as its deficiency leads to disorganized extracellular matrix and tissue morphogenesis defects [8]. In cancers, ELF3 shows dual roles. In gallbladder cancer, it can promote gemcitabine resistance through the PKMYT1/CDK1 signaling pathway, while also suppressing cancer development via down-regulation of the EREG/EGFR/mTORC1 signaling pathway [4,5]. In epithelial ovarian cancer, hypoxia-induced ELF3 promotes tumor angiogenesis through IGF1/IGF1R [6].
In conclusion, ELF3 is a multifunctional gene. Its functions range from regulating plant development in response to environmental cues to influencing various cellular processes and disease progression in animals, especially in cancer. Studies using different genetic models like Arabidopsis and zebrafish, as well as in cancer research, have been crucial in uncovering these diverse functions, providing insights into potential therapeutic strategies for ELF3-related diseases.
References:
1. Jung, Jae-Hoon, Barbosa, Antonio D, Hutin, Stephanie, Jaeger, Katja E, Wigge, Philip A. 2020. A prion-like domain in ELF3 functions as a thermosensor in Arabidopsis. In Nature, 585, 256-260. doi:10.1038/s41586-020-2644-7. https://pubmed.ncbi.nlm.nih.gov/32848244/
2. Ju, Yiheng, Fang, Sheng, Liu, Lei, Ma, Hui, Zheng, Longbo. 2024. The function of the ELF3 gene and its mechanism in cancers. In Life sciences, 346, 122637. doi:10.1016/j.lfs.2024.122637. https://pubmed.ncbi.nlm.nih.gov/38614305/
3. Zhu, Xingzun, Wang, Hongtao. 2024. Revisiting the role and mechanism of ELF3 in circadian clock modulation. In Gene, 913, 148378. doi:10.1016/j.gene.2024.148378. https://pubmed.ncbi.nlm.nih.gov/38490512/
4. Yang, Linhua, Wang, Hui, Guo, Miaomiao, Zhan, Ming, Liu, Yingbin. 2023. ELF3 promotes gemcitabine resistance through PKMYT1/CDK1 signaling pathway in gallbladder cancer. In Cellular oncology (Dordrecht, Netherlands), 46, 1085-1095. doi:10.1007/s13402-023-00799-5. https://pubmed.ncbi.nlm.nih.gov/36988891/
5. Nakamura, Takeharu, Nishikawa, Yoshihiro, Shiokawa, Masahiro, Hatano, Etsuro, Seno, Hiroshi. 2023. ELF3 suppresses gallbladder cancer development through downregulation of the EREG/EGFR/mTOR complex 1 signalling pathway. In The Journal of pathology, 261, 28-42. doi:10.1002/path.6144. https://pubmed.ncbi.nlm.nih.gov/37345534/
6. Seo, Seung Hee, Hwang, Soo-Yeon, Hwang, Seohui, Rho, Seung Bae, Kwon, Youngjoo. 2022. Hypoxia-induced ELF3 promotes tumor angiogenesis through IGF1/IGF1R. In EMBO reports, 23, e52977. doi:10.15252/embr.202152977. https://pubmed.ncbi.nlm.nih.gov/35695065/
7. Zhao, Hang, Xu, Di, Tian, Tian, Zhang, Haisen, Li, Gang. 2020. Molecular and functional dissection of EARLY-FLOWERING 3 (ELF3) and ELF4 in Arabidopsis. In Plant science : an international journal of experimental plant biology, 303, 110786. doi:10.1016/j.plantsci.2020.110786. https://pubmed.ncbi.nlm.nih.gov/33487361/
8. Sarmah, Swapnalee, Hawkins, Matthew R, Manikandan, Priyadharshini, Farrell, Mark, Marrs, James A. 2022. Elf3 deficiency during zebrafish development alters extracellular matrix organization and disrupts tissue morphogenesis. In PloS one, 17, e0276255. doi:10.1371/journal.pone.0276255. https://pubmed.ncbi.nlm.nih.gov/36383615/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen