C57BL/6JCya-Klkb1em1/Cya
Common Name:
Klkb1-KO
Product ID:
S-KO-18613
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Klkb1-KO
Strain ID
KOCMP-16621-Klkb1-B6J-VB
Gene Name
Product ID
S-KO-18613
Gene Alias
APS; Kal-3; Kal3; Klk3; PSA
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Klkb1em1/Cya mice (Catalog S-KO-18613) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000026907
NCBI RefSeq
NM_008455
Target Region
Exon 1~2
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Klkb1, encoding plasma prekallikrein, is also known as Fletcher factor or kallikrein B1. Its physiological role is to catalyze the release of kinins and other vasoactive peptides [6]. It is unique as coagulation factor XI (FXI) arose through a duplication of the Klkb1 gene [2]. Klkb1 is associated with multiple biological processes and diseases, including its role in the coagulation cascade, barrier function, platelet activation, inflammation, and the immune response [2].
In the context of hereditary angioedema, NTLA-2002, an in vivo gene-editing therapy based on CRISPR-Cas9, targets Klkb1. In a phase 1 dose-escalation trial, a single dose of NTLA-2002 led to robust, dose-dependent, and durable reductions in total plasma kallikrein levels, with significant decreases in angioedema attacks at all dose levels, and no severe adverse events were observed [1]. In the phase 2 portion of the trial, single doses of 25 mg or 50 mg of NTLA-2002 reduced angioedema attacks and led to sustained reduction in total plasma kallikrein levels [5]. Additionally, Klkb1 has been associated with HDL-mediated cholesterol efflux capacity in a genome-wide association study [3], may be a potential prognostic biomarker for hepatocellular carcinoma [4], and its polymorphisms are associated with pulmonary thromboembolism in the Chinese Han population [7]. It has also been identified as a potential drug target for keloids [8], and its mRNA expression may be a diagnostic biomarker for chronic lymphocytic leukemia [6].
In conclusion, Klkb1 is crucial for the regulation of various biological functions. Studies using gene-editing approaches targeting Klkb1, like NTLA-2002 in hereditary angioedema, have shown promise in understanding its role in disease and potential therapeutic applications. Its associations with multiple diseases highlight its importance in biomedical research, providing potential targets for drug development and disease diagnosis.
References:
1. Longhurst, Hilary J, Lindsay, Karen, Petersen, Remy S, Lebwohl, David, Cohn, Danny M. . CRISPR-Cas9 In Vivo Gene Editing of KLKB1 for Hereditary Angioedema. In The New England journal of medicine, 390, 432-441. doi:10.1056/NEJMoa2309149. https://pubmed.ncbi.nlm.nih.gov/38294975/
2. Moellmer, Samantha A, Puy, Cristina, McCarty, Owen J T. . Biology of factor XI. In Blood, 143, 1445-1454. doi:10.1182/blood.2023020719. https://pubmed.ncbi.nlm.nih.gov/37874916/
3. Schachtl-Riess, Johanna F, Schönherr, Sebastian, Lamina, Claudia, Köttgen, Anna, Kronenberg, Florian. 2023. KLKB1 and CLSTN2 are associated with HDL-mediated cholesterol efflux capacity in a genome-wide association study. In Atherosclerosis, 368, 1-11. doi:10.1016/j.atherosclerosis.2023.01.022. https://pubmed.ncbi.nlm.nih.gov/36812656/
4. Che, Yi-Qun, Zhang, Yue, Li, Han-Bing, Shen, Di, Cui, Wei. 2021. Serum KLKB1 as a Potential Prognostic Biomarker for Hepatocellular Carcinoma Based on Data-Independent Acquisition and Parallel Reaction Monitoring. In Journal of hepatocellular carcinoma, 8, 1241-1252. doi:10.2147/JHC.S325629. https://pubmed.ncbi.nlm.nih.gov/34676182/
5. Cohn, Danny M, Gurugama, Padmalal, Magerl, Markus, Lebwohl, David, Longhurst, Hilary J. 2024. CRISPR-Based Therapy for Hereditary Angioedema. In The New England journal of medicine, 392, 458-467. doi:10.1056/NEJMoa2405734. https://pubmed.ncbi.nlm.nih.gov/39445704/
6. Adamopoulos, Panagiotis G, Kontos, Christos K, Papageorgiou, Sotirios G, Pappa, Vassiliki, Scorilas, Andreas. 2015. KLKB1 mRNA overexpression: A novel molecular biomarker for the diagnosis of chronic lymphocytic leukemia. In Clinical biochemistry, 48, 849-54. doi:10.1016/j.clinbiochem.2015.04.007. https://pubmed.ncbi.nlm.nih.gov/25891023/
7. Wang, Min-Ne, Xu, Xiao-Mao, Zhai, Zhen-Guo, Fei, Xiao, Guo, Jian. . Association between KLKB1 Polymorphisms and Pulmonary Thromboembolism. In Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae, 37, 274-8. doi:10.3881/j.issn.1000-503X.2015.03.005. https://pubmed.ncbi.nlm.nih.gov/26149136/
8. Wang, Yinmin, Wang, Xiuxia, Yuan, Zhaoqi, Luo, Xusong, Yang, Jun. 2024. Identifying Potential Drug Targets for Keloid: A Mendelian Randomization Study. In The Journal of investigative dermatology, 145, 77-84.e6. doi:10.1016/j.jid.2024.04.023. https://pubmed.ncbi.nlm.nih.gov/38797322/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen