C57BL/6JCya-Snhg1em1/Cya
Common Name:
Snhg1-KO
Product ID:
S-KO-18629
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Snhg1-KO
Strain ID
KOCMP-83673-Snhg1-B6J-VA
Gene Name
Product ID
S-KO-18629
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
19
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Snhg1em1/Cya mice (Catalog S-KO-18629) were purchased from Cyagen.”
Strain Description
Ensembl Number
--
NCBI RefSeq
--
Target Region
Exon 1~10
Size of Effective Region
~3.7 kb
Detailed Document
Overview of Gene Research
Snhg1, short for Small nucleolar RNA host gene 1, is an important member of the SNHG family. This family consists of host genes that can be processed into small nucleolar RNAs and perform significant biological functions. Snhg1, as a long non-coding RNA (lncRNA), is involved in various cellular processes. It has been found to be associated with pathways related to tumorigenesis, cell proliferation, metastasis, and immune cell interaction [1,3,4,5,6,7,8,9,10].
In multiple cancers, Snhg1 shows oncogenic properties. For instance, in breast cancer, knockdown of Snhg1 in macrophages inhibited M2 macrophage polarization by suppressing STAT6 phosphorylation, alleviating MCF-7 cell migration and tube formation of HUVEC, thus reversing the promotion of tumor growth and angiogenesis [2]. In colorectal cancer, Snhg1 knockdown repressed cell growth both in vitro and in vivo. Mechanistically, in the nucleus, it interacted with PRC2 to modulate histone methylation of KLF2 and CDKN2B promoters, and in the cytoplasm, it sponged miR-154-5p to upregulate CCND2 expression [4]. Also, in metastatic colorectal cancer, Snhg1 promoted cell migration and invasion by recruiting HNRNPD to stabilize SERPINA3 mRNA [5]. In neuroblastoma, disrupting endogenous Snhg1 using Nuclease technology suppressed cell proliferation and colony formation ability, and affected the expression of core regulatory circuitry transcription factors, potentially due to interactions between Snhg1 and HDAC1/2 [6].
In conclusion, Snhg1 plays crucial roles in tumor-related biological processes. The findings from loss-of-function experiments, such as knockdown in various cancer cell lines, reveal its oncogenic function in promoting cancer cell growth, metastasis, and in modulating immune-tumor cell interactions. These studies contribute to understanding the mechanisms of cancer development and suggest Snhg1 as a potential biomarker and therapeutic target in cancer [1,2,3,4,5,6,7,8,9,10].
References:
1. Zeng, Huang, Zhou, Shouang, Cai, Weiqiang, Kang, Mingqiang, Zhang, Peipei. 2023. LncRNA SNHG1: role in tumorigenesis of multiple human cancers. In Cancer cell international, 23, 198. doi:10.1186/s12935-023-03018-1. https://pubmed.ncbi.nlm.nih.gov/37684619/
2. Zong, Shoukai, Dai, Wei, Guo, Xiangting, Wang, Kai. 2021. LncRNA-SNHG1 promotes macrophage M2-like polarization and contributes to breast cancer growth and metastasis. In Aging, 13, 23169-23181. doi:10.18632/aging.203609. https://pubmed.ncbi.nlm.nih.gov/34618681/
3. Thin, Khaing Zar, Tu, Jian Cheng, Raveendran, Sudheesh. 2019. Long non-coding SNHG1 in cancer. In Clinica chimica acta; international journal of clinical chemistry, 494, 38-47. doi:10.1016/j.cca.2019.03.002. https://pubmed.ncbi.nlm.nih.gov/30849309/
4. Xu, Mu, Chen, Xiaoxiang, Lin, Kang, Sun, Huiling, Wang, Shukui. 2018. The long noncoding RNA SNHG1 regulates colorectal cancer cell growth through interactions with EZH2 and miR-154-5p. In Molecular cancer, 17, 141. doi:10.1186/s12943-018-0894-x. https://pubmed.ncbi.nlm.nih.gov/30266084/
5. Yang, Huan, Gong, Chunli, Wu, Yuyun, Bai, Jianying, Xiao, Yufeng. 2024. LncRNA SNHG1 facilitates colorectal cancer cells metastasis by recruiting HNRNPD protein to stabilize SERPINA3 mRNA. In Cancer letters, 604, 217217. doi:10.1016/j.canlet.2024.217217. https://pubmed.ncbi.nlm.nih.gov/39233042/
6. Hsu, Chia-Lang, Yin, Chieh-Fan, Chang, Yi-Wen, Huang, Hsuan-Cheng, Juan, Hsueh-Fen. 2022. LncRNA SNHG1 regulates neuroblastoma cell fate via interactions with HDAC1/2. In Cell death & disease, 13, 809. doi:10.1038/s41419-022-05256-z. https://pubmed.ncbi.nlm.nih.gov/36130928/
7. Zhou, Zheng, Chen, Yu. 2023. LncRNA SNHG1 promotes nasopharyngeal carcinoma development via targeting miR-424-5p. In Histology and histopathology, 38, 953-963. doi:10.14670/HH-18-589. https://pubmed.ncbi.nlm.nih.gov/36727428/
8. Fonseca, Tiago S, Martins, Rui Miguel, Rolo, Anabela P, Palmeira, Carlos M. 2024. SNHG1: Redefining the Landscape of Hepatocellular Carcinoma through Long Noncoding RNAs. In Biomedicines, 12, . doi:10.3390/biomedicines12081696. https://pubmed.ncbi.nlm.nih.gov/39200161/
9. Zielske, Steven P, Chen, Wei, Ibrahim, Kristina G, Cackowski, Frank C. 2023. SNHG1 opposes quiescence and promotes docetaxel sensitivity in prostate cancer. In BMC cancer, 23, 672. doi:10.1186/s12885-023-11006-x. https://pubmed.ncbi.nlm.nih.gov/37464317/
10. Zhao, Yanxia, Shi, Jun, Zhao, Yankun, Lu, Zhifang. 2021. SNHG1/miR-186/FUT8 regulates cell migration and invasion in oral squamous cell carcinoma. In Oral diseases, 29, 105-115. doi:10.1111/odi.13878. https://pubmed.ncbi.nlm.nih.gov/33872442/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen