C57BL/6JCya-Mpripem1/Cya
Common Name:
Mprip-KO
Product ID:
S-KO-18632
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mprip-KO
Strain ID
KOCMP-26936-Mprip-B6J-VB
Gene Name
Product ID
S-KO-18632
Gene Alias
9530046C02; Gm34094; RIP3; Rhoip3; mKIAA0864; p116Rip
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mpripem1/Cya mice (Catalog S-KO-18632) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000072031
NCBI RefSeq
NM_201245
Target Region
Exon 4
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Mprip, or Myosin Phosphatase Rho-Interacting Protein, is an F-actin-binding protein. It plays a role in regulating RNA Polymerase II (RNAPII) transcription. Mprip can bind to Phosphatidylinositol 4,5-bisphosphate (PIP2) and is involved in processes related to the condensation and transcription of RNAPII. It is also associated with pathways like the RHO GTPase pathways, which are related to cytoskeletal organization and focal adhesion formation [1,2,3].
In research, depletion of Mprip increases the number of RNAPII initiation condensates, indicating a defect in transcription. This suggests that Mprip regulates the condensation and transcription by influencing the association of the RNAPII complex with nuclear PIP2-rich structures. Additionally, modulation of Mprip-related splicing events is associated with pancreatic ductal adenocarcinoma (PDA) metastases, altered cytoskeletal organization, and the induction of focal adhesion formation [1,2].
In conclusion, Mprip is crucial for regulating RNAPII transcription and is associated with processes such as cytoskeletal organization. Studies, including those involving depletion of Mprip, have revealed its significance in transcription-related functions and in diseases like PDA. Understanding Mprip can provide insights into the mechanisms of transcription regulation and disease development [1,2].
References：
1. Balaban, Can, Sztacho, Martin, Antiga, Ludovica, Harata, Masahiko, Hozák, Pavel. 2023. PIP2-Effector Protein MPRIP Regulates RNA Polymerase II Condensation and Transcription. In Biomolecules, 13, . doi:10.3390/biom13030426. https://pubmed.ncbi.nlm.nih.gov/36979361/
2. Jbara, Amina, Lin, Kuan-Ting, Stossel, Chani, Golan, Talia, Karni, Rotem. 2023. RBFOX2 modulates a metastatic signature of alternative splicing in pancreatic cancer. In Nature, 617, 147-153. doi:10.1038/s41586-023-05820-3. https://pubmed.ncbi.nlm.nih.gov/36949200/
3. Balaban, Can, Sztacho, Martin, Blažíková, Michaela, Hozák, Pavel. 2021. The F-Actin-Binding MPRIP Forms Phase-Separated Condensates and Associates with PI(4,5)P2 and Active RNA Polymerase II in the Cell Nucleus. In Cells, 10, . doi:10.3390/cells10040848. https://pubmed.ncbi.nlm.nih.gov/33918018/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen