C57BL/6JCya-Adam15em1/Cya
Common Name:
Adam15-KO
Product ID:
S-KO-18660
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Adam15-KO
Strain ID
KOCMP-11490-Adam15-B6J-VB
Gene Name
Product ID
S-KO-18660
Gene Alias
AD56; MDC15
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Adam15em1/Cya mice (Catalog S-KO-18660) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029676
NCBI RefSeq
NM_001037722
Target Region
Exon 2~6
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
ADAM15, a disintegrin and metalloproteinase 15, is a transmembrane protein involved in protein ectodomain shedding, cell adhesion, and signaling. It can interact with molecules intra-and extra-cellularly to mediate various cellular functions. ADAM15 affects several important cellular processes, including cell adhesion, degradation of extracellular matrix components, and ectodomain shedding of membrane-bound growth factors, which are relevant to cancer and various inflammatory conditions [3,6].
In disease-related studies, knockdown or knockout of ADAM15 leads to TBEV replication and assembly defects, suggesting its role in the life cycle of tick-borne encephalitis virus [1]. In hepatocellular carcinoma (HCC), ADAM15 is highly expressed, correlated with poor prognosis, and its knockdown promotes apoptosis and suppresses proliferation, migration, and invasion of liver cancer cells [2]. In breast cancer cells, ADAM15 mediates upregulation of Claudin-1 expression [3]. In myocardial infarction, Adam15-/-mice have higher rates of left ventricular rupture, worse left ventricular dysfunction, and reduced collagen cross-linking, indicating ADAM15 is required for optimal collagen cross-linking and scar formation [4]. In prostate cancer, ADAM15 may support disease progression through multiple mechanisms, including promoting metastasis, influencing cell signaling, and angiogenesis [5]. In colorectal tumors, loss of ADAM15 alters the tumor microenvironment, leading to higher immune cell infiltration and cancer cell apoptosis [7]. In pressure overload cardiomyopathy, loss of ADAM15 exacerbates transition to decompensated myocardial hypertrophy and dilation through activation of the calcineurin pathway [8].
In conclusion, ADAM15 is involved in multiple biological processes and plays significant roles in various diseases, such as viral infections, cancers, and heart diseases. Gene knockout mouse models have been crucial in revealing its functions in these specific disease conditions, providing insights into potential therapeutic targets for these diseases.
References:
1. Yang, Qi, Pei, Rongjuan, Wang, Yun, Chen, Xinwen, Chen, Jizheng. 2021. ADAM15 Participates in Tick-Borne Encephalitis Virus Replication. In Journal of virology, 95, . doi:10.1128/JVI.01926-20. https://pubmed.ncbi.nlm.nih.gov/33208450/
2. Xu, Jun Hui, Guan, Yong Jun, Zhang, Yi Chao, Yu, Jia, Wang, Wei Xing. 2021. ADAM15 correlates with prognosis, immune infiltration and apoptosis in hepatocellular carcinoma. In Aging, 13, 20395-20417. doi:10.18632/aging.203425. https://pubmed.ncbi.nlm.nih.gov/34426560/
3. Mattern, Jens, Roghi, Christian S, Hurtz, Melanie, Edwards, Dylan R, Poghosyan, Zaruhi. 2019. ADAM15 mediates upregulation of Claudin-1 expression in breast cancer cells. In Scientific reports, 9, 12540. doi:10.1038/s41598-019-49021-3. https://pubmed.ncbi.nlm.nih.gov/31467400/
4. Chute, Michael, Aujla, Preetinder K, Li, Yingxi, Oudit, Gavin Y, Kassiri, Zamaneh. 2022. ADAM15 is required for optimal collagen cross-linking and scar formation following myocardial infarction. In Matrix biology : journal of the International Society for Matrix Biology, 105, 127-143. doi:10.1016/j.matbio.2021.12.002. https://pubmed.ncbi.nlm.nih.gov/34995785/
5. Lucas, Neali, Day, Mark L. . The role of the disintegrin metalloproteinase ADAM15 in prostate cancer progression. In Journal of cellular biochemistry, 106, 967-74. doi:10.1002/jcb.22087. https://pubmed.ncbi.nlm.nih.gov/19229865/
6. Lucas, Neali, Najy, Abdo J, Day, Mark L. . The therapeutic potential of ADAM15. In Current pharmaceutical design, 15, 2311-8. doi:. https://pubmed.ncbi.nlm.nih.gov/19601833/
7. Puig-Blasco, Laia, Piotrowski, Krzysztof B, Michaelsen, Signe R, Gnosa, Sebastian P, Kveiborg, Marie. 2023. Loss of cancer cell-derived ADAM15 alters the tumor microenvironment in colorectal tumors. In International journal of cancer, 153, 2068-2081. doi:10.1002/ijc.34695. https://pubmed.ncbi.nlm.nih.gov/37602921/
8. Aujla, Preetinder K, Hu, Mei, Hartley, Bridgette, Julien, Olivier, Kassiri, Zamaneh. 2022. Loss of ADAM15 Exacerbates Transition to Decompensated Myocardial Hypertrophy and Dilation Through Activation of the Calcineurin Pathway. In Hypertension (Dallas, Tex. : 1979), 80, 97-110. doi:10.1161/HYPERTENSIONAHA.122.19411. https://pubmed.ncbi.nlm.nih.gov/36330793/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen