C57BL/6JCya-Pfkmem1/Cya
Common Name
Pfkm-KO
Product ID
S-KO-18671
Backgroud
C57BL/6JCya
Strain ID
KOCMP-18642-Pfkm-B6J-VA
Status
When using this mouse strain in a publication, please cite “Pfkm-KO Mouse (Catalog S-KO-18671) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Pfkm-KO
Strain ID
KOCMP-18642-Pfkm-B6J-VA
Gene Name
Product ID
S-KO-18671
Gene Alias
Pfk4, Pfka, Pfkx, PFK-A, PFK-M, Pfk-4, ATP-PFK
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 15
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000051226
NCBI RefSeq
NM_001163487
Target Region
Exon 3~4
Size of Effective Region
~1.8 kb
Overview of Gene Research
PFKM, short for Phosphofructokinase, Muscle, is a key regulatory enzyme in glycolysis. It catalyzes the phosphorylation of fructose-6-phosphate, playing a vital role in energy metabolism pathways. Alterations in PFKM can impact various biological processes and is associated with multiple disease conditions, making it an important gene for functional studies [4,6].
In cancer research, ZEB1 was found to transcriptionally upregulate PFKM, enhancing the Warburg effect and facilitating tumorigenesis and metastasis in hepatocellular carcinoma (HCC). Silencing ZEB1 led to impaired PFKM expression, glycolysis, proliferation, and invasion, which were rescued by exogenous PFKM expression. This indicates the significance of the ZEB1-PFKM axis in HCC carcinogenesis and metastasis [1].
In renal fibrosis, bone marrow mesenchymal stem cell-derived exosomal miR-21a-5p alleviates fibrosis by repressing PFKM expression and attenuating glycolysis in tubular epithelial cells [2].
In osteosarcoma, NAT10-mediated ac4C acetylation drives m6A modification involving YTHDC1-LDHA/PFKM, regulating glycolysis and promoting the disease [3].
In doxorubicin-induced cardiotoxicity, PFKM overexpression inhibits apoptosis, while its silencing promotes apoptosis, with PFKM regulating cell viability and apoptosis through the glycolysis pathway [4].
In sepsis, rTM treatment downregulates PFKM expression in macrophages, protecting mice from sepsis, and HIF-1α regulates PFKM expression through METTL3-mediated m6A modification [5].
In ovarian cancer cells, S-nitrosylation of PFKM at Cys351 by NOS1 stabilizes its tetramer, promoting metabolic reprogramming [7].
In summary, PFKM is crucial for glycolysis and has a far-reaching impact on multiple disease conditions, including cancer, renal fibrosis, osteosarcoma, cardiotoxicity, and sepsis. Gene-knockout or conditional-knockout mouse models (if applicable in these studies) could potentially further clarify its role in these biological processes, providing insights for developing new therapeutic strategies for related diseases.
References：
1. Zhou, Yanming, Lin, Furong, Wan, Tao, Lin, Donghai, Li, Qinxi. 2021. ZEB1 enhances Warburg effect to facilitate tumorigenesis and metastasis of HCC by transcriptionally activating PFKM. In Theranostics, 11, 5926-5938. doi:10.7150/thno.56490. https://pubmed.ncbi.nlm.nih.gov/33897890/
2. Xu, Shihao, Cheuk, Yin Celeste, Jia, Yichen, Shi, Yi, Rong, Ruiming. 2022. Bone marrow mesenchymal stem cell-derived exosomal miR-21a-5p alleviates renal fibrosis by attenuating glycolysis by targeting PFKM. In Cell death & disease, 13, 876. doi:10.1038/s41419-022-05305-7. https://pubmed.ncbi.nlm.nih.gov/36253358/
3. Mei, Zhongting, Shen, Zhihua, Pu, Jiaying, Yuan, Ye, Yang, Lei. 2024. NAT10 mediated ac4C acetylation driven m6A modification via involvement of YTHDC1-LDHA/PFKM regulates glycolysis and promotes osteosarcoma. In Cell communication and signaling : CCS, 22, 51. doi:10.1186/s12964-023-01321-y. https://pubmed.ncbi.nlm.nih.gov/38233839/
4. Zhou, Min, Sun, Xiao, Wang, Chunli, Fang, Chuibi, Hu, Zhenlei. 2022. PFKM inhibits doxorubicin-induced cardiotoxicity by enhancing oxidative phosphorylation and glycolysis. In Scientific reports, 12, 11684. doi:10.1038/s41598-022-15743-0. https://pubmed.ncbi.nlm.nih.gov/35804014/
5. Yao, Chen, Zhu, Hanyong, Ji, Binbin, Pan, Yuchen, Ikezoe, Takayuki. 2024. rTM reprograms macrophages via the HIF-1α/METTL3/PFKM axis to protect mice against sepsis. In Cellular and molecular life sciences : CMLS, 81, 456. doi:10.1007/s00018-024-05489-5. https://pubmed.ncbi.nlm.nih.gov/39549085/
6. Long, Siyu, Zhang, Ran, Yang, Qinni, Zhou, Bin, Zhang, Lin. 2022. Association of PFKM gene polymorphisms and susceptibility to cryptorchidism in a Chinese Han population. In Pediatric surgery international, 38, 1311-1316. doi:10.1007/s00383-022-05167-2. https://pubmed.ncbi.nlm.nih.gov/35838787/
7. Gao, Wenwen, Huang, Mengqiu, Chen, Xi, Hao, Bingtao, Liu, Qiuzhen. 2021. The role of S-nitrosylation of PFKM in regulation of glycolysis in ovarian cancer cells. In Cell death & disease, 12, 408. doi:10.1038/s41419-021-03681-0. https://pubmed.ncbi.nlm.nih.gov/33859186/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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