C57BL/6JCya-Me1em1/Cya
Common Name:
Me1-KO
Product ID:
S-KO-18677
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Me1-KO
Strain ID
KOCMP-17436-Me1-B6J-VB
Gene Name
Product ID
S-KO-18677
Gene Alias
D9Ertd267e; Mdh-1; Mod-1; Mod1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Me1em1/Cya mice (Catalog S-KO-18677) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034989
NCBI RefSeq
NM_008615
Target Region
Exon 4
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Me1, also known as malic enzyme 1, is a cytosolic protein that catalyzes the conversion of malate to pyruvate, generating NADPH from NADP in the process [1]. It is involved in lipid and cholesterol biosynthesis, participates in glycolysis-tricarboxylic acid cycle connection, and is crucial for maintaining redox homeostasis and lipogenesis [1,2]. Early research identified it as an insulin-regulated gene in liver and adipose tissues and a transcriptional target of thyroxine [1].
Global and endothelial-specific Me1 knockout mice were used to study its role in pulmonary hypertension (PH) [2]. The results showed that ME1 protein level and enzymatic activity were highly elevated in lung tissues of patients and mice with PH, mainly in vascular endothelial cells. Global knockout of Me1 protected mice from developing hypoxia-or SU5416/hypoxia-induced PH, and endothelial-specific Me1 deletion similarly attenuated pulmonary vascular remodeling and PH development, suggesting a critical role of endothelial Me1 in PH [2]. In addition, in cancer research, knockdown of Me1 in cancer cells increased intracellular ROS levels, impaired lipogenesis, retarded proliferation, and increased anoikis, while sparing normal cells [3].
In conclusion, Me1 plays essential roles in metabolism-related biological processes, especially in lipid metabolism, redox homeostasis, and lipogenesis. Gene knockout mouse models have revealed its significant contribution to diseases such as pulmonary hypertension and cancer, highlighting its potential as a therapeutic target in these disease areas.
References:
1. Simmen, Frank A, Alhallak, Iad, Simmen, Rosalia C M. . Malic enzyme 1 (ME1) in the biology of cancer: it is not just intermediary metabolism. In Journal of molecular endocrinology, 65, R77-R90. doi:10.1530/JME-20-0176. https://pubmed.ncbi.nlm.nih.gov/33064660/
2. Luo, Ya, Qi, Xianmei, Zhang, Zhenxi, Wang, Jing, Wang, Chen. 2024. Inactivation of Malic Enzyme 1 in Endothelial Cells Alleviates Pulmonary Hypertension. In Circulation, 149, 1354-1371. doi:10.1161/CIRCULATIONAHA.123.067579. https://pubmed.ncbi.nlm.nih.gov/38314588/
3. Shao, Chang, Lu, Wenjie, Du, Ye, Ye, Hui, Hao, Haiping. 2020. Cytosolic ME1 integrated with mitochondrial IDH2 supports tumor growth and metastasis. In Redox biology, 36, 101685. doi:10.1016/j.redox.2020.101685. https://pubmed.ncbi.nlm.nih.gov/32853879/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen