C57BL/6JCya-Adamts10em1/Cya
Common Name:
Adamts10-KO
Product ID:
S-KO-18695
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Adamts10-KO
Strain ID
KOCMP-224697-Adamts10-B6J-VB
Gene Name
Product ID
S-KO-18695
Gene Alias
9430006O18; Adam-ts10; Adamts-10; Znmp
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Adamts10em1/Cya mice (Catalog S-KO-18695) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000087623
NCBI RefSeq
NM_172619
Target Region
Exon 2~5
Size of Effective Region
~4.3 kb
Detailed Document
Overview of Gene Research
ADAMTS10, a secreted metalloproteinase, is crucial for extracellular matrix (ECM) turnover and tissue development. It binds to fibrillin microfibrils, promoting their formation and influencing fibrillin isoform composition. It also plays a role in regulating the bioavailability of TGF-β superfamily growth factors, which are involved in multiple biological processes [1,2,3,4,5]. Genetic models, such as mouse models, have been valuable in studying ADAMTS10's functions.
In Adamts10 -/- mice, ocular microfibrils persist due to reduced fibrillin-2 cleavage, highlighting ADAMTS10's role in clearing ocular fibrillin-2 [2]. In a mouse model with a glaucoma-causative mutation of ADAMTS10 (ADAMTS10G661R/G661R), there is RGC dysfunction, optic nerve axon structural changes, increased apoptosis in the retina during postnatal development, and decreased TGFβ signaling, suggesting ADAMTS10's role in regulating TGFβ signaling [4].
In conclusion, ADAMTS10 is essential for ECM-related functions, especially in microfibril biogenesis and TGFβ signaling regulation. Mouse models, including gene-knockout and mutation-carrying models, have significantly contributed to understanding its role in diseases like glaucoma and Weill-Marchesani syndrome, providing insights into disease mechanisms and potential therapeutic targets.
References:
1. Mead, Timothy J, Apte, Suneel S. 2018. ADAMTS proteins in human disorders. In Matrix biology : journal of the International Society for Matrix Biology, 71-72, 225-239. doi:10.1016/j.matbio.2018.06.002. https://pubmed.ncbi.nlm.nih.gov/29885460/
2. Wang, Lauren W, Kutz, Wendy E, Mead, Timothy J, Reinhardt, Dieter P, Apte, Suneel S. 2018. Adamts10 inactivation in mice leads to persistence of ocular microfibrils subsequent to reduced fibrillin-2 cleavage. In Matrix biology : journal of the International Society for Matrix Biology, 77, 117-128. doi:10.1016/j.matbio.2018.09.004. https://pubmed.ncbi.nlm.nih.gov/30201140/
3. Karoulias, Stylianos Z, Taye, Nandaraj, Stanley, Sarah, Hubmacher, Dirk. 2020. The ADAMTS/Fibrillin Connection: Insights into the Biological Functions of ADAMTS10 and ADAMTS17 and Their Respective Sister Proteases. In Biomolecules, 10, . doi:10.3390/biom10040596. https://pubmed.ncbi.nlm.nih.gov/32290605/
4. Wu, Hang-Jing, Kuchtey, Rachel W, Kuchtey, John. 2022. Optic neuropathy associated with TGFβ dysregulation in mice with a glaucoma-causative mutation of ADAMTS10. In Matrix biology : journal of the International Society for Matrix Biology, 113, 83-99. doi:10.1016/j.matbio.2022.10.001. https://pubmed.ncbi.nlm.nih.gov/36216203/
5. Kutz, Wendy E, Wang, Lauren W, Bader, Hannah L, Keene, Douglas R, Apte, Suneel S. 2011. ADAMTS10 protein interacts with fibrillin-1 and promotes its deposition in extracellular matrix of cultured fibroblasts. In The Journal of biological chemistry, 286, 17156-67. doi:10.1074/jbc.M111.231571. https://pubmed.ncbi.nlm.nih.gov/21402694/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen