C57BL/6JCya-Hsdl2em1/Cya
Common Name:
Hsdl2-KO
Product ID:
S-KO-18702
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Hsdl2-KO
Strain ID
KOCMP-72479-Hsdl2-B6J-VB
Gene Name
Product ID
S-KO-18702
Gene Alias
2610207I16Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hsdl2em1/Cya mice (Catalog S-KO-18702) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000030078
NCBI RefSeq
NM_024255
Target Region
Exon 5~6
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Hsdl2, or hydroxysteroid dehydrogenase-like 2, is a protein-coding gene. It plays a crucial role in lipid metabolism, linking nutritional cues to bile acid and cholesterol homeostasis [1]. It is also involved in regulating cell proliferation, and is associated with various metabolic and cancer-related pathways. Genetic models, such as gene knockout (KO) or conditional knockout (CKO) mouse models, are valuable for studying its functions.
In KO-related studies, Hsdl2 depletion in hepatocytes decreases cholesterol conversion to bile acids, impairs FXR activity, reduces mitochondrial respiration, fatty acid oxidation, and TCA cycle activity, affecting BA metabolism and circulating cholesterol levels upon refeeding [1]. In cancer research, knockdown of Hsdl2 in bladder cancer cells inhibits proliferation and colony formation while promoting apoptosis in vitro and tumor growth inhibition in vivo [2]. In cholangiocarcinoma, Hsdl2 knockdown promotes cancer progression by inhibiting ferroptosis through the P53/SLC7A11 axis [3]. In rectal cancer, Hsdl2 overexpression promotes cell proliferation and cell cycle progression [4]. In pancreatic cancer, silencing Hsdl2 reduces cell proliferation and inhibits lipid metabolism [5]. In lung adenocarcinoma, Hsdl2 knockdown inhibits cell proliferation, invasion, and migration in vitro and tumor growth and metastasis in vivo, and is related to the regulation of AKT2 expression [6]. In papillary thyroid carcinoma, inhibiting Hsdl2 expression suppresses cell proliferation and promotes apoptosis in vitro and in vivo [7].
In conclusion, Hsdl2 is essential in lipid metabolism and cell proliferation regulation. Model-based research, especially KO/CKO mouse models, has significantly contributed to understanding its role in metabolic disorders and various cancers, providing potential therapeutic targets for these disease areas.
References:
1. Samson, Nolwenn, Bosoi, Cristina R, Roy, Christian, Marette, André, Laplante, Mathieu. 2024. HSDL2 links nutritional cues to bile acid and cholesterol homeostasis. In Science advances, 10, eadk9681. doi:10.1126/sciadv.adk9681. https://pubmed.ncbi.nlm.nih.gov/38820148/
2. Jia, Ling-Hua, Hu, Mei-Di, Liu, Yuan, Wang, Jin-Gen, Li, Qiu-Gen. 2019. HSDL2 Promotes Bladder Cancer Growth In Vitro and In Vivo. In International journal of medical sciences, 16, 654-659. doi:10.7150/ijms.31288. https://pubmed.ncbi.nlm.nih.gov/31217732/
3. Ma, Shuoshuo, Ma, Yang, Qi, Feiyu, Lu, Zheng, Zhang, Dengyong. 2023. HSDL2 knockdown promotes the progression of cholangiocarcinoma by inhibiting ferroptosis through the P53/SLC7A11 axis. In World journal of surgical oncology, 21, 293. doi:10.1186/s12957-023-03176-6. https://pubmed.ncbi.nlm.nih.gov/37718459/
4. Cheng, Y, He, X, Wang, L, Hu, J, Hu, J. . [HSDL2 overexpression promotes rectal cancer progression by regulating cancer cell cycle and promoting cell proliferation]. In Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 43, 544-551. doi:10.12122/j.issn.1673-4254.2023.04.06. https://pubmed.ncbi.nlm.nih.gov/37202189/
5. Han, Anna, Xu, Ran, Liu, Ying, Lin, Zhenhua, Yang, Wanshan. 2021. HSDL2 Acts as a Promoter in Pancreatic Cancer by Regulating Cell Proliferation and Lipid Metabolism. In OncoTargets and therapy, 14, 435-444. doi:10.2147/OTT.S287722. https://pubmed.ncbi.nlm.nih.gov/33488098/
6. Shi, Yujia, Mao, Zhengdao, Huang, Yanhua, Huang, Jianan, Zhang, Qian. . Knockdown of HSDL2 inhibits lung adenocarcinoma progression via down-regulating AKT2 expression. In Bioscience reports, 40, . doi:10.1042/BSR20200348. https://pubmed.ncbi.nlm.nih.gov/32211805/
7. Zeng, Jing, Ma, Xiao, Wang, Jinjing, Li, Zhiyuan, Fang, Yi. 2019. Down-regulated HSDL2 expression suppresses cell proliferation and promotes apoptosis in papillary thyroid carcinoma. In Bioscience reports, 39, . doi:10.1042/BSR20190425. https://pubmed.ncbi.nlm.nih.gov/31101684/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen