C57BL/6JCya-Gkn3em1/Cya
Common Name:
Gkn3-KO
Product ID:
S-KO-18707
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Gkn3-KO
Strain ID
KOCMP-68888-Gkn3-B6J-VB
Gene Name
Product ID
S-KO-18707
Gene Alias
1190003M12Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gkn3em1/Cya mice (Catalog S-KO-18707) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000032127
NCBI RefSeq
NM_026860
Target Region
Exon 2~5
Size of Effective Region
~3.6 kb
Detailed Document
Overview of Gene Research
Gkn3, the third member of the gastrokine family, is a gene encoding a secreted protein. Gastrokines are stomach mucus cell-secreted proteins, and Gkn3 may be involved in mucosal protection systems [2,3,4]. It has been associated with processes like gastric atrophy and metaplasia, which are related to an increased risk of gastric adenocarcinoma [1,3].
In mouse models, Gkn3 expression is strongly up-regulated in Tff2-deficient gastric atrophy, a pre-cancerous state. This indicates that Gkn3 might restrain epithelial cell proliferation in this condition [3]. In the context of chronic gastritis in mice, Gkn3 mRNA is a specific marker of mouse gastric corpus metaplasia (SPEM), which was not detectable in healthy gastric corpus but was identified in chronically inflamed stomachs, helping to more accurately assess SPEM [1].
In conclusion, Gkn3 is an important gene in the context of gastric health. Its up-regulation in mouse models of gastric atrophy and its role as a marker of gastric corpus metaplasia in chronic gastritis suggest that it plays a significant role in the development of pre-cancerous gastric conditions. Understanding Gkn3 through these model-based studies may provide insights into the mechanisms underlying gastric diseases and potentially new strategies for prevention and treatment.
References：
1. Bockerstett, Kevin A, Lewis, Scott A, Noto, Christine N, Mills, Jason C, DiPaolo, Richard J. 2020. Single-Cell Transcriptional Analyses Identify Lineage-Specific Epithelial Responses to Inflammation and Metaplastic Development in the Gastric Corpus. In Gastroenterology, 159, 2116-2129.e4. doi:10.1053/j.gastro.2020.08.027. https://pubmed.ncbi.nlm.nih.gov/32835664/
2. Geahlen, Jessica H, Lapid, Carlo, Thorell, Kaisa, Templeton, Alan R, Mills, Jason C. 2013. Evolution of the human gastrokine locus and confounding factors regarding the pseudogenicity of GKN3. In Physiological genomics, 45, 667-83. doi:10.1152/physiolgenomics.00169.2012. https://pubmed.ncbi.nlm.nih.gov/23715263/
3. Menheniott, Trevelyan R, Peterson, Anthony J, O'Connor, Louise, Bell, Katrina M, Giraud, Andrew S. 2010. A novel gastrokine, Gkn3, marks gastric atrophy and shows evidence of adaptive gene loss in humans. In Gastroenterology, 138, 1823-35. doi:10.1053/j.gastro.2010.01.050. https://pubmed.ncbi.nlm.nih.gov/20138039/
4. Salm, Franz, Znalesniak, Eva B, Laskou, Aikaterini, Schlüter, Hartmut, Hoffmann, Werner. 2023. Expression Profiling along the Murine Intestine: Different Mucosal Protection Systems and Alterations in Tff1-Deficient Animals. In International journal of molecular sciences, 24, . doi:10.3390/ijms241612684. https://pubmed.ncbi.nlm.nih.gov/37628863/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen